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EC number: 290-971-9 | CAS number: 90294-83-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2018-03-27 to 2018-08-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- EC Number:
- 290-971-9
- EC Name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- Cas Number:
- 90294-83-0
- Molecular formula:
- CoC32H16-τN8(SO3Na)τ
- IUPAC Name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- Test material form:
- solid: particulate/powder
1
- Specific details on test material used for the study:
- STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
* 0 mg/l (control)
* 1.00 mg/L of test item
* 3.162 mg/L of test item
* 10.0 mg/L of test item
* 31.62 mg/L of test item
* 100 mg/L of test item
- Sampling method: Sampling done at the start of exposure (0 hours) and at the end of exposure (72 hours) with algae.
- Preparation of the samples:
The control and the 3 lowest test item concentrations ( from 1 mg/L to 10 mg/L) were analyzed undiluted.
The two highest test item concentrations (31.62 mg/L and 100 mg/L were diluted with ultra-pure water prior to analysis
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until the start of the analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
A stock solution with a nominal test item concentration of 100 mg/L was freshly prepared with dilution water. The solution was agitated until it was visually clear.
The four other concentrations 1.00 - 3.162 - 10.0 - 31.62 mg/l were prepared by appropriate dilution of the stock solution.
- Control: Six replicates (without test item)
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokierchneriella subcapitata
- Strain: SAG 61.81
- Source (laboratory, culture collection): Sammlung von Algenkulturen (SAG) Pflanzenphysiologisches Institut der Universität Göttingen Nikolausberger Weg 18, D-37073 Göttingen
- Age of inoculum (at test initiation): Three days old
- Method of cultivation:
* Fresh stocks are prepared on Z-Agar.
* Light intensity amounted to 2567 - 5130 lux corresponding to 35 - 70 μE ∙ m-2 ∙ s-1 for 24 hours per day.
* Nutrient medium Z according to LÜTTGE et al. (1994)
* Culture apparatus: Climate room
ACCLIMATION
- Acclimation period: No acclimation period as the preculture was incubated under the same conditions as the test replicates.
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- other: OECD TG 201 medium
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 0.24 mmol Ca+Mg/L
- Test temperature:
- 21 - 24°C, controlled at ± 2°C
- pH:
- 8.1 ± 0.2
- Nominal and measured concentrations:
- Nominal concentration : 1.00 - 3.162 - 10.0 - 3.162 - 100 mg/L of test item
- Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile glass Erlenmeyer flasks (250mL)
- Type : sealed with cotton wool plugs
- Material, size, headspace, fill volume: 250 mL sterile glass Erlenmeyer flasks
- Renewal rate of test solution (frequency/flow rate): no renewal
- Initial cells density: 5523 cells/mL
- Control end cells density: 2370246 cells/mL
- No. of organisms per vessel: 5523 cells/mL
- No. of vessels per concentration (replicates): 3 replicates
- No. of vessels per control (replicates): 6 replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD TG 201 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0 and 72h
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24hours/day light
- Light intensity and quality: Approximately 4440 to 8880 lux, corresponding to 60 to 120 µE*m-2*s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter (Microplate Reader with Software Micro Win 4.41 (MIKROTEC LABORSYSTEME GMBH)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: √10
- Range finding study : yes
- Test concentrations: 1.00 - 10.0 - 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 3.162 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 7.07 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: CI: 5.28 - 8.68 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- ca. 8.38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- CI: 6.55 - 9.22 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- CI: 10.5 - 14.6 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 3.162 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 3.01 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other:
- Remarks:
- CI: 2.16 - 3.99 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- ca. 3.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other:
- Remarks:
- CI: 3.32 - 4.83 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 5.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other:
- Remarks:
- CI: 4.84 - 6.62 mg/L
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid: yes
- EC50: 0.574 mg/L CI: 0.549 - 0.596 mg/L (Growth rate)
- EC50: 0.337 mg/L CI: 0.313 - 0.345 mg/L (Biomass) - Reported statistics and error estimates:
- ErC50 (based on growth rate) is determined via the following the Sigmoidal dose-response (variable slope)
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
EyC50 (based on yield) is determined via the following the Sigmoidal dose-response (variable slope)
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
NOEC/LOEC based growth rate were determined after Wiliams multiple sequential t-test procedure
NOEC/LOEC based on yield were determined after Wiliams multiple sequential t-test procedure
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, the effect values of Additive 8020 on the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours were determined as follows (based on nominal test item concentrations):
The EC50-values for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 11.0 (10.5 - 14.6) mg/L and 5.70 (4.84 – 6.62) mg/L, respectively. The NOEC for growth rate and yield was 3.162 mg/L and 1.00 mg/L, respectively. - Executive summary:
The toxicity of the test item to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201 and Council Regulation (EC) No. 266/2016/Method C.3 from 2018-06-11 to 2018-06-14 at the test facility. The aim of the study was the determination of the effects on growth rate and yield over a period of 72 hours.
The test item is a blue powder and has a water solubility of > 100 mg/L. The study was conducted under static conditions with an initial cell density of 5523 cells/mL. A stock solution and four concentration levels were tested in a geometrical series with a dilution factor of √10: 1.00 - 3.162 - 10.0 - 31.62 - 100 mg/L.
Three replicates were tested for each concentration level and six replicates for the control. The environmental conditions were within the acceptable limits.
The concentrations of the test item were analytically verified via photometric analysis at a wavelength of 662 nm in the fresh media at the start of the exposure (0 hours) and in the old media at the end of the exposure (72 hours) in all concentration levels and in the control.
The measured concentrations of the test item in fresh media at the start of the exposure (0 hours) were in the range of 94 to 105% of the nominal test item concentrations. At the end of the exposure period (72 hours), the measured test item concentrations in the old media were in the range of 96 to 107% of the test item concentrations.
Since the measured concentration of the test item did remain stable within ± 20% of the nominal concentration throughout the exposure period, the nominal concentrations were used for evaluation.
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