Alcohols, C10-16, ethoxylated, sulfates, triethanolammonium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 - 27 Aug 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Please refer to the Category Approach Justification provided in Section 13

Qualifier:

according to guideline

Guideline:

other: EG Guideline 92/69/EWG

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: waterflea
- Strain: Klon 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): neonates
- Method of breeding: isolation of parent daphnids approximately 24 h prior to test start.
- Feeding during test: none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 +/- 1 °C

pH:

7.3 - 7.5

Dissolved oxygen:

7.2 - 8.6 mg/L

Nominal and measured concentrations:

nominal: 5, 9, 15, 26, 45, 80 and 135 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: round bottom glasses
- Material, size, headspace, fill volume: filled with 10 mL test medium
- Aeration: none
- No. of organisms per vessel: 5 (20 animals per concentration)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL per test organism


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium according to Elendt
- Ca/mg ratio: 4 : 1
- Intervals of water quality measurement: test end

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.4 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: endpoint re-calculated from the data given in the study: endpoint in a-WAS and purity of the test material

Details on results:

48h EC50 based on a-WAS = 7.2 mg/L

Concentration [mg/L]	Test duration
	24 h			48 h
	Number mobile	Number immobile	% immobile	Number mobile	Number immobile	% immobile
Control	20	0	0	20	0	0
5	20	0	0	20	0	0
9	19	1	5	4	16	80
15	7	13	65	0	20	100
26	0	20	100	0	20	100
45	0	20	100	0	20	100
80	0	20	100	0	20	100
135	0	20	100	0	20	100

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

27 - 29 Sep 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Please refer to the Category Approach Justification provided in Section 13

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: waterflea
- Strain: clone 5
- Source: in-house culture
- Age at study initiation: approximately 24 h
- Method of breeding: for breeding, females are maintained in M4 medium in 1 L beakers.
- Feeding during test: none


ACCLIMATION
- Acclimation conditions (same as test or not): M4 medium in 1 L beakers
- Type and amount of food: the amount of food, comprising Scenedesmus subspicatus, is such that the amount added is consumed by the animals.
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 +/- 1 °C

pH:

7.7 - 7.8

Dissolved oxygen:

7.9 - 8.1 % (there may be a mistake in the study) - mg/L instead of %

Nominal and measured concentrations:

nominal: 0.4, 0.7, 1.2, 2.0, 3.5, 6.0 and 10 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size: round-bottomed test tubes calibrated to 10 mL
- Aeration: none
- No. of organisms per vessel: 5 (+/-1)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water: 294 mg/L CaCl2 x 2H2O, 123 mg/L MgSO4 x 7 H2O, 63 mg/L NaHCO3, 5.5 mg/L KCl
- Ca/Mg ratio: 4 : 1


OTHER TEST CONDITIONS
- Photoperiod: darkness

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

 

Concentration [mg/L]	Test period (h)
	24 h	48 h
	Immobility [%]	Immobility [%]
Control	5	5
0.4	0	0
0.7^*	5	30
1.2	5	0
2.0	0	0
3.5	0	5
6.0	0	0
10	40	100

^*not used for evaluation, because no effect could be

observed in case the next following concentration

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to the Category Approach Justification provided in Section 13

Reason / purpose for cross-reference:

read-across source

GLP compliance:

yes

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.4 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: endpoint re-calculated from the data given in the study: endpoint in a-WAS and purity of the test material

Remarks:

Source, key, 68891-38-3, 1993b

Details on results:

48h EC50 based on a-WAS = 7.2 mg/L

Description of key information

EC50 (48 h) for Daphnia magna = 7.4 mg a.i./L (nominal, OECD 202) 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

7.4 mg/L

Additional information

No acute invertebrate studies on toxic effects of AES (C12-14, 1-2.5 EO) TEA (CAS 157627-94-6) are available. Therefore, a read-across approach from two structural similar AES (C12-14, 2.5EO) mixtures was applied.

Both studies were conducted according to OECD guideline 202 and GLP with Daphnia magna as test species. In one study the daphnids were exposed for 48 hours to AES (C12-14, 1-2.5EO) Na (CAS 68891-38-3), which resulted in an EC50 value of 7.4 mg a.i./L (nominal) (Sasol, 1993b). In the second study the test organisms were exposed for 48 hours to AES (C12-14, 1-2.5EO) MIPA (CAS 1187742-72-8), which resulted in an EC50 value of 7.7 mg test item/L (nominal) (Sasol, 1999b).