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EC number: 480-460-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- Test article name : Amorphophallus campanulatus dry purified extract
Batch code : 29895/M1
Chemical class : Botanical extract
Preparation date : April, 2009
Expiry date : April, 2011
Physical form at 20°C : Powder
Colour : Pale brown
Storage conditions : The product will be stored at room temperature, in well ventilated room, in its own original container
Safety precautions : Routinely hygienic procedures
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 140 - 250 mg/L as CaCO3
- Test temperature:
- The incubation temperature was continuously monitored during the course of the study by the means of a data logger. The measured values ranged between 20.7 and 22.3 °C during the test period.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
Any other information on results incl. tables
A limit test was performed in accordance with the EU Commission Directive 92/69 to evaluate the effect of the test itemAmorphophallus campanulatusdry purified extract onDaphnia magna.
The daphnids were observed for immobility after 24 and 48 hours of exposure. The observed immobilization in the test concentrations and in the control is reported in Table 2.
Table 2 Lethal effect ofAmorphophallus campanulatusdry purified extract toDaphnia magna
Nominal test item concentration [mg/L] |
No. of |
No. of immobilized |
% of immobilized |
||
24 h |
48 h |
24 h |
48 h |
||
0 (control) |
20 |
0 |
0 |
0 |
0 |
100.0 |
20 |
0 |
0 |
0 |
0 |
In the control 0% immobilization was observed during the test period at each observation time (Table 2). This value complies with the validity criteria of the test, according to the mentioned guidelines.
The results were analyzed for significant differences from control with Fisher’s Exact test (α = 0.05) by the mean of CETIS elaboration software v1.026D.
The NOEC (highest concentration tested without toxic effects after the exposure period) was determined to be equal to the nominal tested concentration, 100 mg/L, at both the observation times.
Given the nature of limit test, the LOEC and the 48-h IC50could not be determined, but, according to the results, were assessed to be greater than the nominal concentration tested (i.e., IC50> 100 mg/L).
No remarkable observations were made concerning the appearance of the test medium. The test medium was a clear solution throughout the whole test duration.
The incubation temperature was in the range 20.7 – 22.3 °C (Figure 1), with a mean value of 21.3 °C and a standard deviation of 0.4 °C. These values slightly exceeded the recommended range (20.0 ± 2.0 °C, however this excess did not affect the results of the study, as showed by the immobilization data in the control.
Fig 1 Trend of temperature during the test period
During the test period, the dissolved oxygen concentrations in the test medium and in the control were always greater than 6.0 mg/L (Table 3). Immediately after preparation, the pH in test solution was 7.55 for the test item and 7.45 for the control and the pH did not vary more than 1 unit during the test, as required by the mentioned guidelines (Table 3).
Table 3 Dissolved oxygen concentrations and pH values in the test media at 0 and after 48 hours from test start of the test period
Nominal test item concentration (mg/L) |
|||
0 (control) |
100.0 |
||
Dissolved oxygen (mg/L) |
0 h |
6.58 |
6.50 |
48 h |
6.54 |
6.30 |
|
pH |
0 h |
7.45 |
7.55 |
48 h |
7.39 |
7.48 |
Applicant's summary and conclusion
- Conclusions:
- The test item Amorphophallus campanulatus dry purified extract caused no adverse effect to Daphnia magna after 48-hours exposure at a nominal product concentration of 100 mg/L under static conditions.
The NOEC, LOEC and IC50 values after 24 and 48 h, calculated based on the nominal concentrations were as follows:
NOEC 100 mg/L
LOEC > 100 mg/L
IC50 > 100 mg/L.
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