Pent-4-enoyl chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2055

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Pent-4-enoic acid

EC Number:

209-732-7

EC Name:

Pent-4-enoic acid

Cas Number:

591-80-0

Molecular formula:

C5H8O2

IUPAC Name:

pent-4-enoic acid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

samples of all concentration levels from freshly prepared media were taken after 0 h and 72 h
samples of all concentration levels of 24 h old media were taken after 24 h and 96 h

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Common name: Danio rerio (zebrafish)
average body weight at test start: 0.13 g
average body lenght at test start: 2.57 cm
fish density less than 1 g per litre test solution
Feeding during test: Food was provided 3 times per week. The amount of food was 4 % of the fish body weight per feeding day. The test fish were
not fed 24 h before test start.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

64 - 77 mg CaCO3/L

Test temperature:

23 +/- 2 °C

pH:

6.0 - 8.5

Dissolved oxygen:

not less than 60 % of air saturation value

Nominal and measured concentrations:

nominal concentration: 6.25 - 12.5 - 25 - 50 - 100 mg/L
measured concentrations: recovery rates of of the test item were > 80 % in all samples in fresh and 24 h old media

Details on test conditions:

Test duration: 96 h
Test vessel: Glass-aquaria loosely covered by glass tops were used.
Test volume: About 10 L per vessel.
Aeration: Gentle aeration was provided.
Test water: Same as for holding.
Number of fish: 7 zebrafish were used per concentration and control.
Loading, length of fish: Fish density in the tanks was than less than 1 g fish per litre test solution.
Average body weight at the test start: 0.13 g
Average body length at the test start: 2.57 cm
Replicates: One replicate per test concentration and control
Introduction of fish: Fish were introduced randomly to individual replicates.
Feeding of test fish: The fish were not fed during the test.
Water temperature: 23 ± 2 °C
Dissolved oxygen concentration: Not less than 60 % of air saturation value.
Light intensity: 0.1 - 10 μmol photons · m-2 · s-1
Photoperiod: A natural photoperiod occurred during the test.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

70.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL 66.0 - 75.8 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

LC50-values and confidence intervals were calculated by probit analyses. Probit values according to WEBER (1986) were used. Calculation of the confidence intervals for LC50 was carried out according to Breitig und Tümpling (1982).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this study 4-Pentenoic acid was found to be toxic to Zebrafish after 96 h at a nominal test item concentration of 100 mg/L and higher.
The LC50 (96 hours) was 70.7 (66.0 - 75.8) mg/L. The NOEC after 96 hours was 50 mg/L (nominal test item concentration).

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