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EC number: 212-338-8 | CAS number: 791-28-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: The test follows the EU Directive 79/831/EEC, Annex V, part C
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: test solution was added without any pretreatment. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Straus
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 hours
- Feeding during test
- Food type: Whilst changing water Brewer's yeast was given, also 1 x daily grown Green algae culture
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.70 +/- 0.5 mmol/L
- Test temperature:
- 18-20 °C
- pH:
- about 8.0
- Dissolved oxygen:
- 7.1 - 8.6 mg/L
- Nominal and measured concentrations:
- The test medium of the highest test concentration, 125 mg/L (nominal) was prepared. Subsequently, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. A control was tested in parallel.
Nominal concentrations: 7.81, 15.6, 31.2, 62.5, 125 mg/mL - Details on test conditions:
- TEST SYSTEM
- Test vessel: flat bottom glass tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline DIN 38412, L 11
- Culture medium different from test medium: Not specified
OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length
EFFECT PARAMETERS MEASURED: mobility; visually after 0, 3, 6, 24, 48 h - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 31.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 42.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 62.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- The EC50 (24-h and 48-h) was determined by the moving avrage method. The EC0 was determined by the highest concentration at which <= 10% immobilisation occurred. The EC0 was determined by the lowest concentration at which 100 % immobilisation occurred.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In an aquatic test with Daphnia magna according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202 the 48-hour EC50 of the test item was determined to be 42.7 mg/L (nominal).
- Executive summary:
The toxicity of the test item to aquatic invertebrates was assessed in a static, 48-hour test according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202. Young daphnids (2 - 24 hours old; 5 animals per test vessel) were exposed to nominal test item concentrations of: 7.81, 15.6, 31.2, 62.5, 125 mg/mL (4 replicates per concentration). A negative control (dilution water only; five replicates) was tested in parallel. The solution water used for the test was prepared according to the test guideline DIN 38412, L 11. Daphnids were fed during the test with Brewer's yeast and green algae (daily). The light regime was 16 light : 8 darkness at a test temperature of 18-20 °C. Mobility of the test animals was checked visually after 0, 3, 6, 24 and 48 hours of exposure. In result, the 48-hour EC0 and EC100 of the test item was determined to be 31.2 mg/L and 62.5 mg/L in nominal, respectively. The 48-hour EC50 of the test item was 42.7 mg/L (nominal).
Reference
Table: Mobile daphnids after 0, 24 and 48 hours of exposure
Concentration [mg/L] | Mobile test animals after | ||
0 h | 24 h | 48 h | |
7.01 | 20 | 20 | 20 |
15.6 | 20 | 20 | 20 |
31.2 | 20 | 20 | 19 |
62.5 | 20 | 5 | 0 |
125 | 20 | 0 | 0 |
0 | 20 | 20 | 20 |
Description of key information
In a test with Daphnia magna according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202, the 48-hour EC50 of the test item was determined to be 42.7 mg/L (nominal).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 42.7 mg/L
Additional information
The toxicity of the test item to aquatic invertebrates was assessed in a static, 48-hour test according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202. Young daphnids (2 - 24 hours old; 5 animals per test vessel) were exposed to nominal test item concentrations of: 7.81, 15.6, 31.2, 62.5, 125 mg/mL (4 replicates per concentration). A negative control (dilution water only; five replicates) was tested in parallel. The solution water used for the test was prepared according to the test guideline DIN 38412, L 11. Daphnids were fed during the test with Brewer's yeast and green algae (daily). The light regime was 16 light : 8 darkness at a test temperature of 18-20 °C. Mobility of the test animals was checked visually after 0, 3, 6, 24 and 48 hours of exposure. In result, the 48-hour EC0 and EC100 of the test item was determined to be 31.2 mg/L and 62.5 mg/L in nominal, respectively. The 48-hour EC50 of the test item was 42.7 mg/L (nominal).
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