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Diss Factsheets
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EC number: 227-774-4 | CAS number: 5977-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 October 2017 - 29 November 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical samples taken at 0 hours (initial value) and 48 hours from aged test solutions were analysed from control and the highest test item
concentration of 100 mg/L. - Vehicle:
- no
- Details on test solutions:
- The necessary amount of test item for preparing the stock solution S1 was weighed on a weighing scoop and transferred to a volumetric flask.
Test medium was added up to the bench mark and the solution was homogenised by shaking. Afterwards the solution was clear and
transparent. Lower test solutions were prepared by dilution of the appropriate solution with test
medium. ~ 50 mL of the prepared solutions were transferred to each test vessel.
Concentration: 100 mg/L, 45.5, 20.7, 9.39, 4.27, 0 mg/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna Straus, Clone V, max. 24 hours old
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 13°dH (232 mg/L CaCO3)
- Test temperature:
- 19.8 ± 0.3 °C
- pH:
- 7.92 ± 0.08 (Std. Dev.)
- Dissolved oxygen:
- 8.9 ± 0.3 mg/L (Std. Dev.)
- Nominal and measured concentrations:
- The initial measured content of acetoacetamide was 97 % of nominal and the aged measured content was 95 % of nominal.
- Details on test conditions:
- Light intensity was ~200 lux at test start
Exposure to light: 16 hours photoperiod /8 hours darkness daily
Feeding: none
Test vessels: four 100 mL glass beakers per concentration, each filled with ~ 50 mL, one additional replicate for pysico -
chemical measurements without test organisms
Loading: ~ 10 mL of test solution for each animal
Aeration: none
Number of animals: 20 per concentration in 4 replicates of 5 - Reference substance (positive control):
- yes
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 24 hours and 48 hours of exposure no immobilisations was observed in the control and up to and including 20.7 mg/L and at the highest test item
concentration of 100 mg/L. No immobilisation higher than the allowed control immobilisation was observed at 45.5 mg/L.
No behavioural changes could be observed. - Reported statistics and error estimates:
- The NOEC was established based on the highest concentration at which the immobilisation is not higher than the allowed control immobilisation
(<=10 % immobilisation). The EC50 was not statistically determined. The EC50 and NOEC-values of daphnids exposed to the test item evaluated
using nominal concentrations. - Validity criteria fulfilled:
- yes
- Conclusions:
- According to the results of the test, the EC50 (48 h) was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
- Executive summary:
A short-term toxicity study to daphnia magna was performed with the test item according to OECD 202 and GLP in year 2017.
According to the results of the test, the EC50 (48 h) was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
Reference
Description of key information
A short-term toxicity study to daphnia magna was performed with the test item according to OECD 202 and GLP in year 2017.
According to the results of the test, the EC50 (48 h) was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
EC 50 > 100 mg/L
NOEC 100 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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