Biphenyl-4-yl-(9,9-dimethyl-9H-fluoren-2-yl)-[2-(9,9-diphenyl-9H-fluoren-4-yl)-phenyl]-amine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016-12-07 - 2017-01-04

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-{[1,1'-biphenyl]-4-yl}-N-[2-(9,9-diphenyl-9H-fluoren-4-yl)phenyl]-9,9-dimethyl-9H-fluoren-2-amine

EC Number:

811-852-5

Cas Number:

1792219-00-1

Molecular formula:

C58H43N

IUPAC Name:

N-{[1,1'-biphenyl]-4-yl}-N-[2-(9,9-diphenyl-9H-fluoren-4-yl)phenyl]-9,9-dimethyl-9H-fluoren-2-amine

Test material form:

solid: particulate/powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Municipal sewage treatment plant, 31137 Hildesheim, Germany
- Pretreatment: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2 hours. Thereafter, the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and maintained in an aerobic condition by aeration with CO2-free air for 7 days. 10 mL/L were used to initiate inoculation.
- Initial cell/biomass concentration: Approx. 10E+7 – 10E+8 CFU/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

18 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

2.89 other: mg O2/mg

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Mineral salts medium according to OECD 301 F
- Test temperature: Nominal: 20 - 24 ± 1 °C; Actual: 20.5 - 21.5 °C
- Continuous darkness: Dark, in an incubator
- pH: 7.63 – 7.89

TEST SYSTEM
- Culturing apparatus: brown glass bottles
- Number of culture flasks/concentration: two for the inoculum control (C1, C2), one for the functional control (R1), two for the test item (P1, P2), one for the toxicity control (T 1)
- Measuring equipment: A rubber sleeve with soda lime was hung into the opening of the bottles to absorb evolved CO2. The bottles were closed with OxiTop® measuring heads and the measuring system was activated.

SAMPLING
- Sampling frequency: The measuring were done at 360 measuring points (every 112 minutes).

CONTROL AND BLANK SYSTEM
- Inoculum blank: The inoculated test medium, consisting of the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were prepared in a measuring flask. 250 ml of this solution will be filled in the brown glass bottles of the inoculum control, using a 250 mL measuring flask.

- Toxicity control: For the toxicity control, the test item was weighed out and directly transferred into the test vessel with 250 ml of the reference item solution, using a 250 mL measuring flask.
- Functional control: The reference item was weighed out and transferred into a measuring flask, the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were added. 250 mL of this solution were filled in the brown glass bottle of the functional control, using a 250 mL measuring flask.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

28 d

Details on results:

In the toxicity control the biodegradation achieved 54% after 14 days. After 28 days the biodegradation came to 56%. The validity criterion that the test item should not inhibit the biodegradation of the reference item was fulfilled.

BOD5 / COD results

Results with reference substance:

The pass level for ready biodegradation (> 60% degradation) was reached on day 4. The validity criterion that the degradation should be > 60% after 14 d was fulfilled.
Stages of Biodegradation: The adaptation phase changed to degradation phase within 2 days (degradation > 10%) and the biodegradation came to a maximum of 80% on day 19.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item was not readily biodegradable within the 28 day period of the study in the Manometric Respiratory Test according to OECD Guideline 301F.

Executive summary:

The ready biodegradability of the test item was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days according to OECD Guideline 301 F at the test facility. The test item concentration selected as appropriate was 18 mg/L, corresponding to a ThOD of 52.0 mg 02/L (53.5 mg 02/L with nitrification) per test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation rate is therefore expressed as the percentage BOD (biological oxygen demand) and was calculated for each study day.

The mean oxygen depletion in the inoculum control was 9.0 mg 02/L on day 28. In order to check the activity of the test system sodium benzoate was used as functional control.

The pass level > 60% was reached on day 4. The biodegradation reached a maximum of 80% degradation on day 19. In the toxicity control containing both test and reference item 54% degradation occurred within 14 days. After 28 days the biodegradation came to 56%. The degradation of the reference item was not inhibited by the test item.

Both test item replicates did not reach the 10% level (beginning of biodegradation) within the 28 day period of the study. The mean biodegradation on day 28 was 1 %.

The validity criteria of the guideline are fulfilled.

The test item is classified as not readily biodegradable within the 28 day period of the study.