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EC number: 911-418-6 | CAS number: 55965-84-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-4 (Fish Early Life-Stage and Aquatic Invertebrate Life-Cycle Studies)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Test material form:
- other: Amber Liquid
- Details on test material:
- - Name of test material (as cited in study report): Kathon™ 886
- Physical state: Amber liquid
- Stability under test conditions: stable at room temperature
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rohm and Haas, Batch No. 24088
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: Stable at room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Insoluble material was not observed in any test vessels during the test
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: No
- Preliminary purification step (if any): No
- Final dilution of a dissolved solid, stock liquid or gel: 0.03, 0.05, 0.08, 0.12, and 0.20 mg a.i./L
OTHER SPECIFICS: Purity of test material was 14.17%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.03, 0.05, 0.08, 0.12, and 0.20 mg a.i./L on days 0, 7, 14 and 21
- Sampling method: Samples were stored in 40 mL glass bottles and transferred immediately to the analytical laboratory
- Sample storage conditions before analysis: Samples were stored at 2-4 ºC for 72 hours or less prior to analysis
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dispersion
- Controls: Negative control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Water
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): Not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain/clone: Daphnia magna
- Source: In-house culture
- Age of parental stock (mean and range, SD): Less than 24 h old at test initiation
- Feeding during test: Yes, yeast/trout chow suspension and Selenastrum capricornutum.
- Food type: Yeast/trout chow suspension and Selenastrum capricornutum
- Amount: Ad libitum
- Frequency: 2 times per day
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- n/a
Test conditions
- Hardness:
- 172 to 180 mg/L as CaCO3
- Test temperature:
- 19.6 +/- 0.6 °C
- pH:
- 8.3 +/- 0.1
- Dissolved oxygen:
- > 75 % saturation (8.7 +/- 0.4)
- Salinity:
- Not applicable
- Conductivity:
- 1100 +/- 100 µmhos/cm
- Nominal and measured concentrations:
- Nominal (mg CMIT/MIT per liter): 0, 0.03, 0.05, 0.08, 0.12, and 0.20
Mean measured (mg CMIT/MIT per liter): 0, 0.02, 0.04, 0.06, 0.10 and 0.18 - Details on test conditions:
- TEST SYSTEM
- Test vessel: Flow-through
- Renewal of test solution: 24.6 media exchanges per 24 h in each test vessel
- Volume of test vessels: 270 mL
- Volume/animal: 27 mL
- Number of animals/vessel: 10
- Number of vessels/ concentration: 4
- Test performed in closed vessels due to significant volatility of TS: No
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water collected at EnviroSystems, Hampton, New Hampshire, USA
- Alkalinity: 155 to 175 mg/L as CaCO3
- Hardness: 172 to 180 mg/L as CaCO3
OTHER TEST CONDITIONS
- Photoperiod: 16 h light :8 h dark with 15 minute transition periods.
- Light intensity: 8 Es-1m-2
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: No
- Observations on body length and weight: No
- Other biological observations: No
- Mortality of control: 2.5% after 21 days
- Other adverse effects control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Mean measured concentrations of the TS were in agreement with nominal concentrations of active ingredient. Insoluble material was not observed in any test vessels during the test.
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- a
- Reported statistics and error estimates:
- Results of the toxicity test were interpreted by standard statistical techniques (US EPA, 1988b, Short-term methods for estimating the chronic toxicity of effluents and receiving waters to marine and estuarine organisms. Environmental Monitoring and Support Laboratory, Cincinnati, Ohio, USA, EPA/600/4-87/028), when warranted. Shapiro-Wilk’s test was used to determine if data were normally distributed, and Bartlett’s test was used to determine if variances were homogeneous. Since the assumption of homogeneity of variance was met for all data analyzed, a parametric ANOVA followed by Dunnett’s test was used to compare control to treatment means. Dichotomous data were transformed (arc sin square root) prior to statistical analysis. All calculations were performed using mean measured concentrations of the active ingredient.
Any other information on results incl. tables
Effect data
Mean measured concentration (mg CMIT/MIT per liter) |
% survival at 21 days |
Total number of young produced |
Day of first brood |
Mean young per surviving female |
Average dry weight of surviving adults (mg) |
0 (control) |
97.5 |
989 |
9 |
102 |
0.60 |
0.02 |
92.5 |
993 |
9 |
108 |
0.64 |
0.04 |
92.5 |
968 |
9 |
106 |
0.67 |
0.06 |
87.5 |
1015 |
9 |
116 |
0.66 |
0.10 |
82.5 |
1177 |
9 |
144 |
0.66 |
0.18 |
62.5 * |
898 |
9 |
148 |
0.73 |
* statistically different from control
Biological endpoint |
NOEL |
LOEL |
MATC |
EC50 |
|
(mg CMIT/MIT per liter) |
|||
First generation survival |
0.10 |
0.18 |
0.13 |
>0.18 |
Time to first brood |
0.18 |
>0.18 |
>0.18 |
-- |
Number of young produced 1. total per replicate 2. mean per surviving female |
0.18 0.18 |
>0.18 >0.18 |
>0.18 >0.18 |
-- |
Dry weight of first generation |
0.18 |
>0.18 |
>0.18 |
-- |
Validity criteria for invertebrate reproduction test according
|
Fulfilled |
Not fullfilled |
Mortality of parent animals < 20 % at test termination |
yes |
|
Mean number of live offspring produced per parent animal surviving at test termination³60 |
Yes |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results are based on mean measured concentrations:
21d-NOEC = 0.10 mg a.s./l;
21d-LC50 > 0.18 mg a.s./l. - Executive summary:
US EPA FIFRA 72- 4, Aquatic invertebrate life- cycle studies with analytical confirmation of TS concentrations. Insoluble material was not observed in any test vessels during the test. Mean measured concentrations of the TS were in agreement with nominal concentrations of active ingredient. Loading rate during the toxicity test was approximately 0.024 g/L at any time and 0.001 g/L/ 24 hours. Water quality parameters were within acceptable limits throughout the test. The percentage of surviving adults was statistically significantly reduced in comparison to the control at 0.18 mg CMIT/MIT per liter. The total number of young producted and the mean number of young per surviving female was not significantly lower than the control at any tested concentration. The average dry weight of surviving adult daphnids was not lower than the control at any concentration and the time to first brood was identical for all concentrations of TS. No sublethal effects were noted during the test. The most sensitive biological endpoint was survival of first generation daphnids
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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