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Diss Factsheets
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EC number: 259-201-9 | CAS number: 54528-00-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 19 Feb. 2010 to 02 Mar. 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The following instruments and devices were used for this study:
- Automatic pipettes with one-way tips, LAUS No.: 31
- Glass measuring cylinders and flasks, 100 and 1000 ml
Test solutions
- Vehicle:
- no
- Details on test solutions:
- On the day of the start of the test, a stock solution containing 1 g/L test item was prepared. This solution was used to prepare three treatments containing 1, 10 and 100 mg/L.
Medium used: chlorine-free tap water, full composition analysed.
Medium renewal: twice a week
Dilution water : Good quality drinking water is used. The composition is analysed yearly by the supplier.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Danio rerio HAMILTON-BUCHANAN
sexually immature young fish, length 2 ± 1 cm
The animals were obtained from a commercial supplier. Before being used for the test, the fish were kept for 14 days under test conditions. During this period, mortality didn't surpass 5 %.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 1.18 mmol/L
- Test temperature:
- 24.2°C at T0
24.9°C at T24
25.2°C at T48
25.0°C at T72
25.2°C at T96 - pH:
- Between 7.9 and 8.3 at T0 hours
Between 7.6 and 7.8 at T96 hours - Dissolved oxygen:
- between 8.9 mg/L and 9.6 mg/L at T0
between 8.4 mg/L and 8.8 mg/L at T96 - Salinity:
- Not specified (freshwater used)
- Conductivity:
- 142 S/cm at 20°C
- Nominal and measured concentrations:
- Nominal concentration : 0 ; 1 ; 10 & 100 mg/L
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 - < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mortalities
The mortalities were recorded daily and can be found in the following table.
Table of mortalities:
Nom. Conc. in mg/L
Total of fish used
dead fish
24 h
48 h
72 h
96 h
cum
cum.
cum.
cum.
0
7
0
0
0
0
1
7
0
0
0
0
10
7
0
0
6
7
100
7
7
7
7
7
No abnormal behaviour of the surviving fish was observed.
pH and O2 Values, Temperature
The pH values in the test media and the control ranged from 7.5 to 8.3. The concentration of dissolved oxygen stayed above 6.9 mg/L or 78.9 % throughout the test. Temperature range was 24.2 — 25.2°C. The details are given in the following tables:
Table of pH and O2Values
Nom. Conc.
pH at t in h
O2 in mg/L at t in h
mg/L
0h
24 h
48 h
72 h
96 h
0h
24 h
48 h
72 h
96 h
0
7.9
7.5
7.5
7.6
7.6
8.9
8.7
8.5
8.8
8.8
1
7.9
7.5
7.5
7.6
7.6
9.6
8.3
8.3
8.1
8.4
10
8.0
7.5
7.5
7.7
7.7
9.6
8.5
8.6
8.1
8.7
100
8.3
7.8
7.6
7.6
7.8
9.6
8.4
6.9
7.8
8.4
Table of temperatures
0 h
24 h
48 h
72 h
96 h
24.2
24.9
25.2
25.0
25.2
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All validity criteria were met.
The test item 3-AMINO 3-AZABICYCLO(3,3,0)OCTANE ("AMINAZA PURIFIEE") showed mortality in treatments 10 and 100 mg/L.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test is considered valid. - Executive summary:
The toxicity against Danio rerio was tested using a static test design.
The main study was performed using three concentrations containing 1, 10 and 100 mg/L. Two concentrations showed mortality (86 and 100 %).
The following results for the test item 3-AMINO 3-AZABICYCLO(3,3,0)OCTANE ("AMINAZA PURIFIEE") could be determined:
96h NOEC = 1.0 mg/L
96h LC50 = 1 - 10 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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