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EC number: 285-749-3 | CAS number: 85136-25-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening test, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- no
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Remarks:
- 100 mL of secondary effluent of a urban sewage treatment plant / L
- Duration of test (contact time):
- 28 d
- Details on study design:
- TEST CONDITIONS:
- Duration of the test: 28 days
- Test temperature: 22 ± 2 ºC
- Fotoperiod: difuse light
- Type of flasks: conic flask of 2,5 L / volume used 1,5L
- Number of culture flasks/concentration: 2
- Initial concentration of the study: 10 mg/L of TOC
- Final concentration of the study: 30 mg/L of TOC
- Reference substance: sodium acetate
- Inoculum: 100 mL of secondary effluent of a urban sewage treatment plant / L
- Composition of medium:
Solution 1:
KH2PO4 8,5 g/L H2O
K2HPO4 21,75 g/L H2O
Na2HPO4 2H2O 33,4 g/L H2O
NH4Cl 0,5 g/L H2O
Solution 2: CaCl2 2H2O 36,4 g/L H2O
Solution 3: MgSO4 7H2O 22,5 g/L H2O
Solution 4: FeCl3 6H2O 0,25 g/L H2O
Add to 1000 mL of deionized water: solution 1: 10 mL; solución 2: 1 mL; solución 3:1 mL; solución 4:1 mL
DEVELOPMENT OF THE STUDY:
- 800 ml of mineral medium are introduced into 2.5-liter conical flasks and sufficient volumes of the mother liquors of the test substance and of reference are added to the individual flasks to obtain a substance concentration equivalent to 10-30 mg toc / L.
Taking into account that 1 g / L of THE product (0.75 g / L of active material) has a TOC value of 314.2 mg / L, a solution of the product of 31.8 mg / L (23, 85 mg / L of active material) is prepared to obtain a TOC value of 10 mg / L, and a product solution of 95.5 mg / L (71.63 mg / L of active material) to obtain a TOC value of 30 mg / L.
- The pH is adjusted to 7.4.
- The flasks are inoculated with effluent of wastewater in the proportion of 100 ml / L.
- An inoculum control is prepared with the mineral medium, but without substance or reference substance.
- All flasks are flushed at 1.5 L with mineral medium and, after shaking, a sample is taken from each flask to determine the initial concentration of total dissolved organic carbon (TOC).
- The flasks are covered with aluminum foil, so as to allow free circulation of air between the flask and the surrounding atmosphere.
- Next, the flasks are placed in the agitator to start the test.
- Throughout the study, the concentrations of TOC in each flask are determined in duplicate at time intervals of 2-3 days. For each determination, only the minimum necessary volume of problem suspension is taken.
- Before taking the samples, it is necessary to compensate the losses that have been produced by evaporation of the test medium in the flasks, adding the necessary amount of dilution water. Before taking samples, the culture medium is well shaken and is made sure that the material that has adhered to the walls of the containers dissolves or resuspens.
- The samples are filtered by membrane immediately after taking them. Each filtered sample is analyzed using a TOC analyzer. - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 3
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item Direct Blue 281 (batch: PF-11895) shows no biodegradation after 28 days of testing.
- Executive summary:
The determination of the biodegradability of Direct Blue dye 281 (batch: PF-11895) was carried out following the OECD Guideline 301A (1992) and the Spanish Royal Decree 363/1995 (BOE No. 133 of June 1995).
Biodegradation is defined as the decomposition of an organic compound by the biological action of microorganisms, which results in the disappearance of the original molecular structure and the formation of smaller organic molecules.
The starting point is a known concentration of the test substance (10-40 mg TOC / L) and the follow-up is performed by analysis of Total Dissolved Organic Carbon (TOC) at time intervals over a period of 28 days.
The results are expressed as a percentage of biodegradation after 28 days.
The result obtained in this test indicates that the Direct Blue 281 dye does not show biodegradation after 28 days of testing.
Reference
% of biodegradation of Direct Blue 281 along the time
% biodegradation | |||
Direct Blue 281 | |||
time | sodium acetate | 10 ppm TOC | 30 ppm TOC |
0h | 0 | 0 | 0 |
day 1 | 52.7 | 0 | 0 |
day 2 | 86.3 | 1 | 0 |
day 5 | 97,4 | 2 | 1 |
day 7 | 100 | 3 | 2 |
day 9 | 100 | 3 | 2 |
day 12 | 100 | 3 | 2 |
day 14 | 100 | 3 | 2 |
day 16 | 100 | 3 | 2 |
day 19 | 100 | 3 | 2 |
day 21 | 100 | 3 | 2 |
day 23 | 100 | 3 | 2 |
day 26 | 100 | 3 | 2 |
day 28 | 100 | 3 | 2 |
Description of key information
One test according to OECD Guideline 301A conducted on the substance shows that it is not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
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