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EC number: 245-589-7 | CAS number: 23328-87-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Nov 2017 - 31 Jan 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesanstalt für Umwelt, Messungen und Naturschutz Baden-Württemberg, Karlsruhe, Germany
Test material
- Reference substance name:
- 2-heptadecyl-1H-imidazole
- EC Number:
- 245-589-7
- EC Name:
- 2-heptadecyl-1H-imidazole
- Cas Number:
- 23328-87-2
- Molecular formula:
- C20H38N2
- IUPAC Name:
- 2-heptadecyl-1H-imidazole
1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Analytical samples were taken from the highest test item concentration and control at test start and after 24 hours from fresh and aged solutions and after 48 hours from aged solutions. For each sampling also a retain sample was taken.
- Sample storage conditions before analysis: All samples were stored deep frozen until they were transferred to the analytical laboratory.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by directly weighing 100 mg in 1000 mL test medium. This stock solution was stirred in the dark at room temperature for 24 h (based on OECD Series on Testing and Assessment No. 23). Afterwards the test item had not gone into solution completely. Small, evenly distributed particles were visible within the solution and particles were observed at the bottom of the vessel and on the surface of the solution. Insoluble particles of the test item were separated from the liquid phase by filtration through a filter with 0.22 µm mean pore size which was saturated beforehand. The test item solutions were made by diluting the stock solution with test medium to give the required nominal test item concentrations. The preparation procedure was repeated after 24 hours. Around 50 mL of the prepared solutions were transferred to each test vessel.
- Controls: blank control without the test item
- Eluate: no
- Differential loading: no
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): The filtrated solution was clear and transparent.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Continuously bred in the laboratory and originally purchased in a healthy condition from the Federal Environment Agency in Berlin/Germany.
- Age: Freshly hatched daphnids less than 24 hours old were used for the test.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 13°dH (232 mg/L CaCO3)
- Test temperature:
- 19.1 ± 0.4 °C
- pH:
- 7.89 ± 0.13
- Dissolved oxygen:
- 8.9 ± 0.4 mg O2/L
- Nominal and measured concentrations:
- nominal: control, 0.0100, 0.100, 1.00, 10.0, 100 mg/L
measured: < LOD for all treatment solutions - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass vessel (100 mL), were filled up with ~ 50 mL test solution. The test units were covered with a glass plate.
- Renewal rate of test solution: The test solutions were freshly prepared at 0 and 24 h.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
Elendt M4 standard medium was used as test medium. At test initiation the pH-value of the control (untreated test medium) was 7.91, the dissolved oxygen concentration was 9.0 mg/L and the total hardness was 13°dH (232 mg/L as CaCO3).
- Intervals of water quality measurement: The test temperature and the pH-value as well as the oxygen concentration of the test solutions were measured at all loading rates and control at t = 0 h fresh and t = 24 h from fresh test solutions and after t = 24 h and t = 48 h from aged test solutions in one separate replicate per test item loading rate without test organisms.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod /8 hours darkness daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Immobility after 24 and 48 h
- Behavioural changes of daphnids were recorded at 24 and 48 hours after starting the test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- filtered
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- filtered
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0%
- Behavioural changes: No behavioural changes were observed.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No; Test solutions were clear and colorless - Results with reference substance (positive control):
- - Results with reference substance valid?
yes
- EC50 (24 h) = 1.00 - 2.00 mg/L
Any other information on results incl. tables
Table 1: Determined concentration of the test substance
Test item nominal [mg/L] |
Sampling [h] |
Test substance |
|
[mg/L] |
% of nominal |
||
Control |
0 fresh |
< LOD |
- |
|
24 aged |
< LOD |
- |
|
24 fresh |
< LOD |
- |
|
48 aged |
< LOD |
- |
100 |
0 fresh |
< LOD |
- |
|
24 aged |
< LOD |
- |
|
24 fresh |
< LOD |
- |
|
48 aged |
< LOD |
- |
- = not calculated; LOD = 0.00150 mg/L test substance; LOQ = 0.00500 mg/L test substance
Table 2: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
Control: 0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
≥ 7.7 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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