Oxydiethylene bis(chloroformate)

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1968

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Documentation insufficient for assessment. Due to different testing conditions (occlusive instead of semi aocclusive) different exposure times (1, 5, 15 min and 20 h instead of 4 h) the study is, hoever, not directly comparable to an OECD TG 405.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1968

Materials and methods

Principles of method if other than guideline:

2 rabbits were treated for 1, 5 and 15 minutes and 2 other animals for 20 hours using occlusive conditions. 0.5 ml test substance is applied onto the intact skin.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Oxydiethylene bis(chloroformate)

EC Number:

203-430-9

EC Name:

Oxydiethylene bis(chloroformate)

Cas Number:

106-75-2

Molecular formula:

C6H8Cl2O5

IUPAC Name:

oxydiethane-2,1-diyl dichlorocarbonate

Details on test material:

- Name of test material (as cited in study report): Diglycoldichlorkolensäureester
- Physical state: liquid
- Analytical purity: no data
- Other: pH 1; Melting point +4 °C; Boiling point 199 °C

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler, Offenbach, Germany
- Weight at study initiation: 2.14 kg (male); 2,32 kg (male)

ENVIRONMENTAL CONDITIONS
no data

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 500 µl

Duration of treatment / exposure:

1, 5, and 15 minutes; 20 hours

Observation period:

24 h and 8 days after treatment.

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: 2.5 * 2.5 cm application to the dorsal skin.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): 50 % Lutrol solution
- Time after start of exposure: 1, 5, 15 min post application, the 20 h exposure was not washed.

SCORING SYSTEM: descriptive scoring system comparable to draize score used in OECD TG 404

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 h

Score:

1.5

Max. score:

4

Reversibility:

fully reversible

Remarks on result:

other: 20 h occlusive

Results 2

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: 20 h occlosive

Irritant / corrosive response data:

still moderate to strong eschar visible 8 days after application

Any other information on results incl. tables

The results indicate a strong potential for skin irritation

Exposition:	20 h
Animal	Reading	Erythema	Edema	Comment
1	24 h	2	3
2	24 h	2	0
1	48 h	2	0
2	48 h	2	0
1	8 d	2	0	eschar formation; anemic, slight suprafacial necrosis
2	8 d	2	0	eschar formation
mean	24 - 48 h	2.0	0
Exposition:	15 min
Animal	Reading	Erythema	Edema	Comment
1	24 h	2	0
2	24 h	1	0
1	48 h	2	0
2	48 h	2	0
1	8 d	0	0	eschar formation
2	8 d	0	0	eschar formation
mean	24 - 48 h	1.75	0
Exposition:	5 min
Animal	Reading	Erythema	Edema	Comment
1	24 h	2	0
2	24 h	1	0
1	48 h	2	0
2	48 h	1	0
1	8 d	0	0	eschar formation
2	8 d	0	0
mean	24 - 48 h	1.50	0
Exposition:	1 min
Animal	Reading	Erythema	Edema	Comment
1	24 h	2	0
2	24 h	1	0
1	48 h	2	0
2	48 h	2	0
1	8 d	0	0	eschar formation
2	8 d	0	0	eschar formation
mean	24 – 48 h	1.75	0