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EC number: 275-640-9 | CAS number: 71566-55-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Jul 2016 to 18 Aug 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: International Standard ISO 9439; Water Quality - Evaluation in an aqueous medium of the "ultimate" aerobic biodegradability of organic compounds - Method by analysis of released carbon dioxide.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium bis[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxy-N-3-(isopropoxypropyl)benzenesulphonamidato(2-)]cobaltate(1-)
- EC Number:
- 275-640-9
- EC Name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium bis[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxy-N-3-(isopropoxypropyl)benzenesulphonamidato(2-)]cobaltate(1-)
- Cas Number:
- 71566-55-7
- Molecular formula:
- C44H50CoN10O10S2.C28H31N2O3
- IUPAC Name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium bis[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxy-N-3-(isopropoxypropyl)benzenesulphonamidato(2-)]cobaltate(1-)
- Test material form:
- solid
- Details on test material:
- - Physical state / Appearance: Solid / red
- Total Organic Carbon: 612 mg/g
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage conditions: At room temperature
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source: Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany.
- Preparation of inoculum: The inoculum was collected on 18 July 2016 from the aeration tank of the plant. A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. To reduce the content of inorganic carbon in the blank controls the activated sludge was aerated with carbon dioxide free air for about 48 hours at 22 ± 2 °C. At the day of exposure the suspension was washed one time with drinking water. Therefore the aeration was stopped and the sludge was allowed to settle. After settling the supernatant was discarded and the remaining sludge suspension was filled up with drinking water and the concentration oft the sludge was adjusted to 6.0 g/L dry weight. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
- Initial conc.:
- 33.5 mg/L
- Based on:
- test mat.
- Remarks:
- replicate 1
- Initial conc.:
- 32.8 mg/L
- Based on:
- test mat.
- Remarks:
- replicate 2
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- PREPARATION OF TEST ASSAYS
The test assays were prepared at the day of exposure. First, the required volumes of deionised water and the solutions of mineral salts were dosed to all test vessels. For preparation of the test vessels with test substance, the required amounts of the test substance aliquots for a test concentration of 20 mg/L TOC were weighed onto small glass plates (microscope cover slips) and completely added with the glass plates to the vessels of the test substance assays and to the vessel of the inhibition control. Because of poor water solubility of test substance these test assays were treated for few minutes in an ultrasonic bath to ensure an even distribution of test substance in test medium.
TEST CONDITIONS
- Composition of medium: 15 mL solution A, 1.5 mL solution B, 1.5 mL solution C and 1.5 mL solution D was used for the preparation of the test assays.
1. Solution A: KH2PO4 : 8.50 g; K2HPO4 : 21.75 g; Na2HPO4 × 2 H2O : 33.40 g; NH4Cl : 0.50 g. The compounds were dissolved with deionised water to 1000 mL; the pH value was adjusted to 7.4.
2. Solution B: CaCl2 × 2 H2O : 36.40 g. The compound was dissolved with deionised water to 1000 mL.
3. Solution C: MgSO4 × 7 H2O : 22.50 g. The compound was dissolved with deionised water to 1000 mL.
4. Solution D: FeCl3 × 6 H2O : 0.25 g. The compound was dissolved with deionised water to 1000 mL.
- Temperature: 22 + 2 °C
- pH adjusted: Yes, to 7.4 ± 0.2, if necessary.
- Aeration: Yes, with carbon dioxide free air 800 mL/h
- Suspended solids concentration: 30 mg/L dry weight.
- Stirring: The test assays were stirred using magnetic stirrers.
TEST SYSTEM
- Culturing apparatus: 2 L incubtion bottles, filled up to a volume of 1.5 L
- Number of culture flasks: 2
SAMPLING
- Sampling frequency: Usually twice a week
- Sampling method: At the end of exposure, the pH values were measured in each test vessel. For stripping of carbon dioxide, dissolved in the test medium, each test vessel was acidified by adding 2 mL of concentrated hydrochloric acid. For determination of the decrease of DOC samples were taken from the test vessels of the blank control and from the test vessel of the reference substance control and the DOC content was determined after centrifugation (approx. 15 minutes at 4000 rpm). The concentration of dissolved organic carbon in the blank controls and reference substance assays were determined. Since the test substance was insufficiently soluble in water, no DOC-measurements could be performed from the test assay of the inhibition control and from the test substance test assays. The aeration was continued for about 24 hours and the released carbon dioxide amounts in both traps of each test vessel were determined and added to the calculated amount of the previous day.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 assays (BC)
- Inhibition control: 1 assay (IH)
- Reference substance: 1 assay (RS)
Reference substance
- Reference substance:
- aniline
- Remarks:
- 20 mg/L TOC
Results and discussion
- Test performance:
- - Measured DIC-concentrations in the blank controls at begin of exposure (mean value): 1.0 mg/L
- Amount of produced CO2 in the blank controls at the end of exposure (mean value): 31.4 mg/L
- Deviation of the degree of biodegradation of the test substance in the plateau phase was <20%
- The degree of biodegradation of the reference substance was >60% CO2/ThCO2 after 14 days
- The degree of biodegradation in the inhibition control was >25 % CO2/ThCO2 after 14 days
- The content of DIC in the blank control at start of exposure at the test concentration of 20 mg/L TOC was not <1 mg/L
- The amount of produced CO2 in the inoculum blank (“blank controls”) at the end of exposure (mean value) was <70 mg/L
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 28 d
- Details on results:
- During the contact time of 28 days, no biodegradation of the test substance was observed. For more information, see 'Any other information on results incl. tables'; The degree of biodegradation of the inhibition control was determined to be 30% after 14 days.
BOD5 / COD results
- Results with reference substance:
- The degree of biodegradation for the reference substance was 84% after 14 days.
Any other information on results incl. tables
Table: Degree of Biodegradation; [% CO2/ThCO2]
Test duration [days] |
RS |
IH |
TS1 |
TS2 |
TS mv |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
0 |
-1 |
-1 |
-1 |
-1 |
5 |
34 |
13 |
-3 |
-3 |
-3 |
7 |
50 |
20 |
-2 |
-2 |
-2 |
14 |
84 |
30 |
-3 |
-1 |
-2 |
19 |
94 |
32 |
-4 |
0 |
-2 |
21 |
97 |
33 |
-5 |
0 |
-3 |
23 |
98 |
33 |
-6 |
0 |
-3 |
26 |
100 |
34 |
-7 |
0 |
-4 |
28 |
101 |
35 |
-9 |
-1 |
-5 |
RS: reference substance assay
IH: inhibition control assay
TS: test substance assay
mv: mean value
Table: Produced carbon dioxide amount in the test vessels
[mg CO2/test vessel] |
mg CO2 added up after substraction of the mean value of the blank controls |
||||||||
Duration of exposure [days] |
BC mv |
RS |
IH |
TS1 |
TS2 |
RS |
IH |
TS1 |
TS2 |
2 |
4.2 |
3.7 |
2.8 |
2.9 |
3 |
-0.5 |
-1.4 |
-1.3 |
-1.2 |
5 |
7.2 |
45.1 |
37.8 |
4.6 |
5.4 |
37.4 |
29.2 |
-3.9 |
-3 |
7 |
3.3 |
21.1 |
17.6 |
4.4 |
4.4 |
55.2 |
43.5 |
-2.8 |
-1.9 |
14 |
8 |
45.3 |
30.1 |
7.2 |
9.3 |
92.5 |
65.6 |
-3.6 |
-0.6 |
19 |
6.4 |
16.8 |
12 |
5 |
7 |
102.9 |
71.2 |
-5 |
0 |
21 |
3.2 |
6.2 |
4.5 |
2.2 |
3.4 |
105.9 |
72.5 |
-6 |
0.2 |
23 |
3 |
4.7 |
3.6 |
2.3 |
3.2 |
107.6 |
73.1 |
-6.7 |
0.4 |
26 |
3.8 |
5.5 |
5 |
2.4 |
3.9 |
109.3 |
74.3 |
-8.1 |
0.5 |
28 |
2.8 |
4.3 |
3.9 |
2.4 |
2.8 |
110.4 |
77.4 |
-10.6 |
-0.6 |
29 |
5.2 |
4.8 |
7.2 |
3.1 |
4.1 |
|
|
|
|
BC: blank control assay
See table above for the other abbreviations
Measured data: TIC [mg/L]
Day |
|
BC NaOH |
BC1 |
BC2 |
RS |
IH |
TS1 |
TS2 |
|
a |
1 |
|
|
|
|
|
|
0 |
b |
1 |
- |
- |
- |
- |
- |
- |
|
a |
0.9 |
12.8 |
12.1 |
11.1 |
8.7 |
8.9 |
9.3 |
2 |
b |
1 |
13 |
12.3 |
11.3 |
8.8 |
9 |
9.3 |
|
a |
0.7 |
19.7 |
21.3 |
124 |
104 |
13.6 |
15.6 |
5 |
b |
0.7 |
19.9 |
21.4 |
124 |
104 |
13.2 |
15.6 |
|
a |
0.7 |
8.5 |
11.2 |
58.4 |
48.9 |
13 |
13.1 |
7 |
b |
0.9 |
8.7 |
11.2 |
58.6 |
48.9 |
12.8 |
12.9 |
|
a |
0.6 |
28.8 |
15.7 |
124 |
82.9 |
20.6 |
26 |
14 |
b |
0.7 |
29.1 |
16.1 |
124 |
82.7 |
20.2 |
25.9 |
|
a |
0.7 |
23.4 |
13.2 |
46.9 |
33.5 |
14.2 |
19.8 |
19 |
b |
0.6 |
23.2 |
12.8 |
46.5 |
33.7 |
14.4 |
19.9 |
|
a |
0.8 |
10.5 |
7.9 |
17.6 |
13 |
6.9 |
9.9 |
21 |
b |
0.8 |
10.7 |
8 |
17.6 |
12.6 |
6.5 |
9.9 |
|
a |
0.7 |
9.6 |
8.3 |
13.6 |
10.6 |
6.9 |
9.3 |
23 |
b |
0.7 |
9.5 |
8.1 |
13.4 |
10.5 |
6.7 |
9.3 |
|
a |
0.8 |
13.2 |
9.2 |
15.8 |
14.3 |
7.5 |
11.3 |
26 |
b |
0.9 |
13.2 |
9.4 |
15.8 |
14.3 |
7.4 |
11.4 |
|
a |
0.6 |
8.7 |
8 |
12.3 |
11.3 |
7.2 |
8.3 |
28 |
b |
0.7 |
8.7 |
8 |
12.7 |
11.4 |
7.4 |
8.4 |
flask 1 |
a |
|
15.7 |
9.4 |
11.2 |
17.1 |
6.6 |
9.8 |
29 |
b |
15.4 |
9.6 |
11.1 |
16.8 |
6.4 |
9.6 |
|
flask 2 |
a |
3.5 |
3.1 |
3.4 |
4.1 |
3.5 |
3 |
|
29 |
b |
3.4 |
3.1 |
3.4 |
4 |
3.5 |
2.9 |
See tables above for abbreviations
The NaOH blank value is determined on filling the absorption vessels.
The measured values, for instance applies on day 0 for the first and the second sampling.
The NaOH blank value was subtracted from each test vessel.
Applicant's summary and conclusion
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance is not readily biodegradable (according to OECD criteria). It is poorly biodegradable.
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