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EC number: 240-505-5 | CAS number: 16455-61-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-04-28 - 2010-06-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- State Office for the Environment, Water and Trade Control, Mainz, Germany
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
- Name of test material (as cited in study report): Acetic acid,oxo-,sodium salt (FeNaEDDHA)
-Synonym: Acetic acid,oxo-,sodium salt, reaction products with ethylenediamine and phenol,iron sodium salts
- Physical state: Solid/ red, dark
- Analytical purity: 100 % (UVCB)
- Storage condition of test material: At ambient temperature under exclusion of light - Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analyzed. The samples were transported to the Analytical Laboratory on the day of sampling.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance was weighed in (119.69 mg) and made up with 1000 mL M4 medium. The mixture was stirred on a magnetic stir plate for about 10 minutes in the dark at 20 ± 2 degree C.
All test solutions were visibly clear red colored over the exposure period. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institute National de Recherche Chimique Applique, France, in 1978.
From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation: < 24 hours (at least 3rd brood progeny)
- Feeding during test: Daily
- Food type: Unicellular algae Desmodesmus subspicatus, cultured continuously at the test facility - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observation period reported.
- Hardness:
- 2.20 – 3.20 mmol/L CaCO3
- Test temperature:
- 19.5 – 19.9 °C (continuous monitoring)
- pH:
- 7.5 – 8.5
- Dissolved oxygen:
- >= 3 mg/L
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- 0 and 120 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Aeration: approximately 24 hours prior to use
- Renewal rate of the culture water: Twice weekly
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 replicates with test animals (5 animals per replicate)
- Total No. of animals per concentration: 20
TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: 2.20 – 3.20 mmol/L
- Ca/mg ratio: about 4 : 1
- Conductivity: 550 - 650 uS/cm
OTHER TEST CONDITIONS
- Photoperiod/Light intensity: The test was performed in the dark due to the potential light sensitivity of the test substance.
EFFECT PARAMETERS MEASURED: Each test vessel was visually checked for immobilized daphnids after 0, 24 and 48 hours. In addition any abnormal behaviour or appearance was documented. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Since the analytically determined concentrations of the test substance in the test solutions were within +/- 20 % of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50: The EC50(24h) of the reference substance potassium dichromate was 1.19 mg/L.
- Other: This result is within the range of 0.6 – 2.1 mg/L (ISO 6341) and indicates that the culture of Daphnia magna used in this study is responding normally to toxic stress. - Reported statistics and error estimates:
- Since the study is designed as a limit test, no statistical analysis was performed.
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria of the test guideline were fulfilled.
- Conclusions:
- In conclusion the 48 hour EC50 values for FeNaEDDHA in this acute toxicity study with Daphnia magna were > 120 mg/L based on the nominal concentration of the test substance and > 122 mg/L based on the mean measured concentrations.
- Executive summary:
This acute toxicity study investigated the effect of Fe(Na)EDDHA on the immobilisation of Daphnia magna according to the principles of OECD Guideline 202 and EU Method C.2 (Salinas, 2010). Juvenile daphnids (younger than 24 hour old) were exposed to one concentration of the test substance and compared to a control. Immobilisation of daphnia is determined after 24 and 48 hours of exposure. The test substance was was tested at 120 mg/L. The 48 hour EC50 value for the test substance was greater than 120 mg/L based on the nominal concentration of the test substance and greater than 122 mg/L based on the mean measured concentrations. Since the analytically determined concentrations of the test substance in the test solutions were within +/- 20% of the nominal concentrations, the effect concentration is expressed relative to the nominal concentration for the evaluation of the test substance.
Reference
Number of mobile daphnids
Nominal concentration [mg/L] |
Replicate 1 [h] |
Replicate 2 [h] |
Replicate 3 [h] |
Replicate 4 [h] |
∑ |
∑ |
||||||||
0 |
24 |
48 |
0 |
24 |
48 |
0 |
24 |
48 |
0 |
24 |
48 |
24 |
48 |
|
0 (control) |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20 |
20 |
120 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20 |
20 |
Effect concentrations after 24 hours and 48 hours of exposure. The EC values based on nominal concentrations were:
Time |
EC0 |
EC50 |
Confidence limits 95% |
EC100 |
24 |
>120 |
> 120 |
* – * |
> 120 |
48 |
>120 |
> 120 |
* – * |
> 120 |
*The data were insufficient to determine confidence limits.
Description of key information
EC50(48h): > 120 mg/L (nominal), EC50(48h): > 122 mg/L (measured) [OECD 202; Daphnia magna, read-across substance: Fe(Na)EDDHA]
Key value for chemical safety assessment
Additional information
Ecotoxicological effects towards aquatic invertebrates of o-o-EDDHA (CAS16455-61-1) was not investigated experimentally. Read across from the structurally related substance Fe(Na)EDDHA (CAS 84539-55-9) was performed to fulfil this relevant endpoint under REACH (for further details, please refer to the separate read-across statement).
An acute toxicity study investigated the effect of Fe(Na)EDDHA on the immobilisation of Daphnia magna according to the principles of OECD Guideline 202 and EU Method C.2 (Salinas, 2010). Juvenile daphnids (younger than 24 hour old) were exposed to one concentration of the test substance and compared to a control. Immobilisation of daphnia was determined after 24 and 48 hours of exposure. The test substance was tested at 120 mg/L. The 48 hour EC50 value for Fe(Na)EDDHA with Daphnia magna was greater than 120 mg/L based on the nominal concentration of the test substance and greater than 122 mg/L based on the mean measured concentrations. Since the analytically determined concentrations of the test substance in the test solutions were within +/- 20% of the nominal concentrations, the effect concentration is expressed relative to the nominal concentration for the evaluation of the test substance.
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