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EC number: 249-352-9 | CAS number: 28983-56-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Data is from publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute Toxicity of Food additives Tartrazine and carmoisine on white male Mice
- Author:
- Lamia A. M. Ai-Mashhedy, Ali N. Fijer
- Year:
- 2 016
- Bibliographic source:
- International Journal of Pharm Tech Research,Vol.9, No.4, pp 364-367, 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- Acute oral toxicity study was conducted on male mice to determine LD50 value of chemical Carmoisine.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
Test material
- Reference substance name:
- Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalenesulphonate
- EC Number:
- 222-657-4
- EC Name:
- Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalenesulphonate
- Cas Number:
- 3567-69-9
- Molecular formula:
- C20H12N2Na2O7S2
- IUPAC Name:
- disodium 4-hydroxy-3-[(4-sulfonato-1-naphthyl)diazenyl]naphthalene-1-sulfonate
- Details on test material:
- - IUPAC Name of test material: disodium 4-hydroxy-3-[(4-sulfonato-1-naphthyl)diazenyl]naphthalene-1-sulfonate
- Common Name: Carmoisine
- Molecular formula: C20H14N2O7S2.2Na
- Molecular weight: 502.4338 g/mole
- Substance type: Organic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - IUPAC Name of test material: disodium 4-hydroxy-3-[(4-sulfonato-1-naphthyl)diazenyl]naphthalene-1-sulfonate
- Common Name: Carmoisine
- Molecular formula: C20H14N2O7S2.2Na
- Molecular weight: 502.4338 g/mole
- Substance type: Organic
- Physical state: Solid
Test animals
- Species:
- mouse
- Strain:
- other: Sprague- Dawley white
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: college of science at the University of Babylon
- Age at study initiation:8-9 weeks old
- Weight at study initiation: 25-30 gm
- Fasting period before study:
- Housing: animals were housed in polyacrylic cages, not more than three animals per cage under standard laboratory condition
- Diet (e.g. ad libitum): standard diet
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22±2C°
- Humidity (%):50-55%
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): 12 hrs. Light: dark cycle
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- not specified
- Doses:
- 1250 mg/kg ,2500 mg/kg ,3750 mg/kg ,5000 mg/kg , 6250 mg/kg
- No. of animals per sex per dose:
- 1250 mg/kg- 6 animals
2500 mg/kg-6 animals
3750 mg/kg-6 animals
5000 mg/kg-6 animals
6250 mg/kg-6 animals - Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration:3 days
- Frequency of observations and weighing:No data available
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: The mice were observed for 3 days for the signs and symptoms of toxicity as well as the death rate of each group were recorded
- mice in the control group received distilled water - Statistics:
- The LD50 of the substances was calculated using the arithmetic method of Karber
The LD50 was calculated using the following formula:
LD50 =LDy – Σ (Dd x md)/N
Where LDy =Highest dose (LD100)
N =Number of animals per group
Dd =Dose difference
Md =Mean dead
Results and discussion
- Preliminary study:
- not specified
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 4 166.66 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 50% mortality was observed.
- Mortality:
- 50% mortality was observed.
- Clinical signs:
- other: The clinical signs such as, loss of appetite, drowsiness, tachycardia, decrease in locomotion and anorexia were distinctive signs observed in the mice before dead.
- Gross pathology:
- not specified
- Other findings:
- not specified
Any other information on results incl. tables
Determination of LD50 value of carmoisine in mice:
Dose |
No.of mice in group |
No. of death |
Mean death |
Dose difference |
Mean death *dose diff. |
1250 mg/kg |
6 |
0 |
0 |
0 |
0 |
2500mg/Kg |
6 |
0 |
0 |
1250 |
0 |
3750/mg/Kg |
6 |
2 |
1 |
1250 |
1250 |
5000mg/kg |
6 |
5 |
3.5 |
1250 |
4387 |
6250mg/kg |
6 |
6 |
3.5 |
1250 |
6875 |
LD50 = LDy-Σ(Dd×md) /N = 6250 -12500/6 = 4166.66 |
12500 |
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- The acute oral toxicity dose (LD50) was considered to be 4166.66 mg/kg bw, when male Sprague- Dawley white mice were treated with Disodium 4-hydroxy-3-[(4-sulfonato-1-naphthyl)diazenyl]naphthalene-1-sulfonate (CAS no: 3567-69-9) for 3 days via oral gavage route.
- Executive summary:
Acute oral toxicity study was conducted in male Sprague-Dawley white mice by using test chemical disodium 4-hydroxy-3-[(4-sulfonato-1-naphthyl)diazenyl]naphthalene-1- sulfonate (CAS no: 3567-69-9) at the concentrations of 1250 mg/kg, 2500 mg/kg, 3750 mg/kg, 5000 mg/kg, 6250 mg/kg bw. 6 animals/dose were administered the given test chemical via oral gavage route and control animals were received distilled water. The mice were observed for 3 days for the signs and symptoms of toxicity as well as the death rate of each group were recorded. The LD50 of the substances was calculated using the arithmetic method of Karber. 50% mortality was observed at 4166.66 mg/kg bw. The clinical signs such as, loss of appetite, drowsiness, tachycardia, decrease in locomotion and anorexia were distinctive signs observed in the mice before dead. Therefore, LD50 was considered to be 4166.66 mg/kg bw, when male Sprague- Dawley white mice were treated with Disodium 4-hydroxy-3-[(4-sulfonato-1-naphthyl)diazenyl] naphthalene-1-sulfonate for 3 days via oral gavage route.
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