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EC number: 260-280-7 | CAS number: 56602-77-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 January 1991 to 14 June 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: MITI as described in "Chemical Substances Control Law" (Japanese Law No. 117,1974)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Activated sludge, which was purchased from a reputable supplier and was cultured in accordance with SOP T-201, was used for the test.
Concentration of activated sludge: 30 mg/L. - Duration of test (contact time):
- 14 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- TOC removal
- Details on study design:
- CULTIVATION
Cultivation of test solutions for the biodegradability test was carried our according to the following conditions:
Concentration of test material: 100 mg/L
Concentration of activated sludge: 30 mg/L
Cultivating temparature: 25 °C
Cultivating period: 14 days
ACTIVATED SLUDGE
Activated sludge, which was purchased from Chemical Inspection & Testing Institute, Japan on January 17, 1991 and was cultured in accordance with SOP T-201, was used for the test.
CONCENTRATION OF SUSPENDED SOLIDS IN THE ACTIVATED SLUDGE (MLSS)
MLSS: 4808 mg/L
Volume of activated sludge solution added to each test vessel: 1.87 mL
INSTRUMENT FOR CULTIVATION
Closed system oxgen consumption measuring apparatus No.2 (Coulometer : Ohkura Electric Co.,Ltd.). There was no abnormality of function of this apparatus when inspections (periodic and before-using) were carried out.
INITIATION OF CULTIVATION
Following test solution were prepared and cultivated under the aforementioned cultvating conditions:
1. Sludge + aniline
2. Control blank
3. Water + test material (1)
4. Water + test material (2)
5. Sludge + test material (1)
6. Sludge + test material (2)
RECOVERY TEST
Duplicate test solutions of (sludge + test substance) (water + test substance) for recovery test were prepared by reference to the above. Simultaneously duplicate control blanks which did not contain the test substance were prepared in a similar manner. Test vessels were closed with glass plugs and sealed with grease. The test solutions were mixed for 1 hour with a stirrer.
After test solutions were pre-treated by the method above, and analysed according to the procedures above. - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- 0
- Sampling time:
- 14 d
- Details on results:
- Percentages degradations calculated on the basis of SOP T-208 are shown below:
Percentage degradation by BOD:
Test substance = 0 %
Aniline:
63.2 % at 7th day
>75 % at 14th day
Percentage degradation by HPLC:
No.1 peak component = 1.74 %
No.2 peak component = 0.95 %
No.3 peak component = 0 %
No.4 peak component = 0 %
Percentage degradation by TOC = 0 % - Parameter:
- BOD5
- Value:
- 0 other: %
- Results with reference substance:
- Aniline:
Day 7 = >40 %
Day 14 = >65 % - Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Percentage biodegradations of aniline calculated from BOD were over 40 % and 65 % at the 7th and 14th day, respectively. Therefore, this test condition was valid. Percentage biodegradations of test material calculated fron BOD and TOC were both 0 %. In HPLC Method, percentage biodegradations for peak components of No.1 and No. 2 of test material were approxinately 1~ 2 %, on the other hand, other conponents were 0 %.
Consequently it was concluded that the test substance was almost not biodegraded by microorganisms. - Executive summary:
The ready biodegradability of the test material was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)).
Under the conditions of the study, the test material attained 0 % degradation, as calculated from BOD and TOC. Furthermore, in the HPLC method the test material attained 0 - 2 % degradation.
It is subsequently concluded that the substance is not readily biodegradable.
Reference
Results of biodegradation test
Number |
Sample |
Initial content (mg) |
TOD (mg) |
BOD (mg)
7 days 14 days |
Percentage biodegradation 7 days 14 days |
||
1 |
Aniline |
30 |
90.3 |
61.5 |
>75 |
63.2 |
74.2 |
2 |
Control blank |
0 |
0 |
4.4 |
7.4 |
0.0 |
0.0 |
3 |
Test substance + water -1 |
29.7 |
95.6 |
0.9 |
1.9 |
- |
- |
4 |
Test substance + water - 2 |
30.9 |
99.6 |
0.8 |
1.7 |
- |
- |
5 |
Test substance + sludge - 1 |
30.9 |
99.5 |
0.7 |
1.5 |
0.0 |
0.0 |
6 |
Test substance + sludge - 2 |
30.7 |
98.9 |
1.2 |
2.6 |
0.0 |
0.0 |
Test substance concentration (mg/L): 100
Activated sludge concentration (mg/L): 30
Temperature (°C): 25 ± 1
TOD of test substance: 3.22 (g/g)
Description of key information
Not readily biodegradable, OECD 301C, M Sato (1991).
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of the test material was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)).
Under the conditions of the study, the test material attained 0 % degradation, as calculated from BOD and TOC. Furthermore, in the HPLC method the test material attained 0 - 2 % degradation.
It is subsequently concluded that the substance is not readily biodegradable.
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