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Diss Factsheets
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EC number: 806-984-5 | CAS number: 1392411-89-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From November 22, 2016 to November 25, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- see 'Any other information on materials and methods'
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- see 'Any other information on materials and methods'
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- DOC determination using a TOC analyser
- Details on sampling:
- At the start and at the end of the test, the content of the test substance in the test solutions was determined using DOC determination.
- Vehicle:
- no
- Details on test solutions:
- For the 1st experiment individual water-accommodated fractions (WAF) were prepared for the test. This was done by mixing the nominal loads with the corresponding amount of algal medium (demineralised water enriched with minerals but without algae) and shaking vigorously for 23 h. The resulting solutions were filtered through 0.45 μm nylon filters. For the 2nd experiment a water-accommodated fraction (WAF) at 100 mg/L nominal load was prepared. This was done by mixing 100 mg/L of test substance with the corresponding amount of algal medium (demineralised water enriched with minerals but without algae) and shaking vigorously for 24 h. The resulting solution was filtered through 0.45 μm nylon filters. The lower concentrations were prepared by serial dilution of this stock solution.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Unicellular freshwater green alga.
Genus: Desmodesmus;
Species: subspicatus;
SAG Strain Number: 86.81;
Taxonomic position: Chlorophyta - Chlorophyceae;
Origin and Culture: The culture of Desmodesmus subspicatus was obtained in January, 2016 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8°C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- not specified
- Test temperature:
- 22.4 – 22.5°C
- pH:
- 7.6 - 7.8
- Dissolved oxygen:
- not specified
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- First experiment in mg/L:
Nominal: 1.0; 3.2; 10.0; 32.0; 100.0
Measured: 1.1; 3.4; 10.2; 35.2; 101.6
Second experiment in mg/L:
Nominal: 100.0
Measured: 101.6 - Details on test conditions:
- In the first experiment: 1.0 to 100 mg/L (nominal);
In the second experiment: treatment concentrations were prepared from serial dilution of a 100 mg/L (nominal) solution - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 8.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- In the first experiment inhibition in all tested concentrations was within the same range and no dose response could be observed. Therefore, the study was repeated.
- Results with reference substance (positive control):
- Potassium dichromate (K2Cr2O7) was used as positive control in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 72 h nominal EC50 and NOEC of the substance to Desmodesmus subspicatus were determined to be 51 mg/L and 3.2 mg/L (based on growth rate) respectively.
- Executive summary:
A study was conducted to determine the toxicity of the substance to Desmodesmus subspicatus according to OECD Guideline 201 and EU Method C.3, in compliance with GLP. Two valid experiments were performed. Individual water-accommodated fractions (WAF) were prepared for the test. The study was conducted using 5 test concentrations ranging from 1.0 to 100 mg/L (nominal), with an incubation time of 72 h. The measured concentrations (determined by measurement of dissolved organic carbon, DOC) were comparable to the nominal ones. The cell concentration of each replicate was determined by measuring the cell numbers every 24 h with an electronic particle counter. Growth rate and yield were determined from the cell number at the respective observation time. Significant inhibition of algal growth was observed at all concentrations, except the lowest concentration of 1 mg/L (nominal). Under the study conditions, the 72 h nominal EC50 and NOEC of the substance to Desmodesmus subspicatus were determined to be 51 mg/L and 3.2 mg/L (based on growth rate) respectively (Muckle, 2017).
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 51 mg/L
- EC10 or NOEC for freshwater algae:
- 3.2 mg/L
Additional information
A study was conducted to determine the toxicity of the substance to Desmodesmus subspicatus according to OECD Guideline 201 and EU Method C.3, in compliance with GLP. Two valid experiments were performed. Individual water-accommodated fractions (WAF) were prepared for the test. The study was conducted using 5 test concentrations ranging from 1.0 to 100 mg/L (nominal), with an incubation time of 72 h. The measured concentrations (determined by measurement of dissolved organic carbon, DOC) were comparable to the nominal ones. The cell concentration of each replicate was determined by measuring the cell numbers every 24 h with an electronic particle counter. Growth rate and yield were determined from the cell number at the respective observation time. Significant inhibition of algal growth was observed at all concentrations, except the lowest concentration of 1 mg/L (nominal). Under the study conditions, the 72 h nominal EC50 and NOEC of the substance to Desmodesmus subspicatus were determined to be 51 mg/L and 3.2 mg/L (based on growth rate) respectively (Muckle, 2017).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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