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EC number: 201-941-1 | CAS number: 89-80-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge nitrification inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from Peer reviewed journal
- Justification for type of information:
- Data is from Peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Version / remarks:
- No data
- Principles of method if other than guideline:
- Toxicity of menthone on the microorganisms Pseudomonas citronellolis DSM 50332 was studied for 21 days.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one- Common name: Menthone - Molecular formula: C10H18O- Molecular weight: 154.2512 g/mol- Smiles notation: C1([C@@H](CC[C@@H](C1)C)C(C)C)=O- InChl: 1S/C10H18O/c1-7(2)9-5-4-8(3)6-10(9)11/h7-9H,4-6H2,1-3H3- Substance type: Organic- Physical state: Liquid
- Analytical monitoring:
- yes
- Details on sampling:
- No data available
- Vehicle:
- yes
- Test organisms (species):
- other: Pseudomonas citronellolis DSM 50332
- Details on inoculum:
- - Laboratory culture: Pseudomonas citronellolis DSM 50332 was obtained from the Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany. - Name and location of sewage treatment plant where inoculum was collected: Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany.- Method of cultivation: no data - Preparation of inoculum for exposure: Pure cultures were isolated anaerobically on agar bottle plates in which the substrate was provided by diffusion from a reservoir through the gas phase to the agar surface (10, 19). During all manipulations, the bottles containing the agar were flushed with N2-CO2 (90:10, vol/vol) by using a Hungate syringe. A 20-ml portion of liquid anoxic medium containing 1.5% washed agar was prepared with chelated trace elements instead of non-chelated trace elements (20) and with 50 mM carbonate instead of the phosphate-carbonate buffer above, dispensed into a 100-ml bottle, and solidified quickly by placing the bottle on a plastic bag filled with ice. After inoculation, the agar bottles were inverted, and 75 ml of monoterpene in 3 ml of HMN was added to each bottle. During incubation, the bottles were kept horizontal with the agar aloft. Direct contact between the organic phase and the agar was avoided- Pretreatment: no data- Initial biomass concentration: no data
- Test type:
- other: Agar diffusion method.
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks on exposure duration:
- Test conducted for 10 days to 21 days.
- Post exposure observation period:
- No data available
- Hardness:
- No data
- Test temperature:
- mesophilic temperature
- pH:
- 7
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal
- Details on test conditions:
- - Test vessel: Glass bottels- Material, size, headspace, fill volume: 0.5 liter of glass bottles that contained 400 ml of medium, 4 ml of HMN, and 200 ml of liquid substrate- Biomass loading rate: 0.5-liter bottles that contained 400 ml of medium, 4 ml of HMN, and 200 ml of liquid substrate or 200 mg of solid substrate under an N2-CO2 (90:10, vol/vol). A 100-ml portion of the water-mud mixture from the ditch was inoculated into each bottle together with 350 ml of medium, 8 ml of HMN, and 400 ml or 400 mg of substrate. - Photoperiod: performed in dark
- Reference substance (positive control):
- no
- Remarks:
- other: Pasteurized samples and inoculated preparations without nitrate or monoterpene were used as controls.
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 308 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of anaerobic gas production
- Remarks on result:
- other: No inhibition was observed
- Details on results:
- No data available
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- No data available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of no growth inhibition of microorganisms Pseudomonas citronellolis due to the menthone for 10 days to 3 weeks, the NOEC was 308 mg/l (2mM)
- Executive summary:
Toxicity of menthone on the growth of microorganisms Pseudomonas citronellol is DSM 50332 for 21 days. Analytical grade samples were used for the test. Test performed in 0.5 liter of agar bottle plates by using the pure culture of microorganism providing proper temperature and PH. Culture were inoculated in dark having the mesophilic temperature. Growth inhibition of Pseudomonas citronellolis was observed at 2m M of concentration of menthone for minimum 10 days to 3 weeks. Analytical grade samples were used for the test. Thus after the exposure with chemical no inhibition was observed. On the basis of no growth inhibition of microorganisms Pseudomonas citronellolis due to the menthone for 10 days to 3 weeks, the NOEC was 308 mg/l (2mM).
Reference
Description of key information
Toxicity of menthone on the growth of microorganismsPseudomonas citronellol isDSM 50332 for 21 days. Analytical grade samples were used for the test. Test performed in 0.5 liter of agar bottle plates by using the pure culture of microorganism providing proper temperature and PH. Culture were inoculated in dark having the mesophilic temperature. Growth inhibition of Pseudomonas citronellolis was observed at 2m M of concentration of menthone for minimum 10 days to 3 weeks. Analytical grade samples were used for the test. Thus after the exposure with chemical no inhibition was observed. On the basis of no growth inhibition of microorganisms Pseudomonas citronellolis due to the menthone for 10 days to 3 weeks, the NOEC was 308 mg/l (2mM).
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 308 mg/L
Additional information
Toxicity of menthone on the growth of microorganisms Pseudomonas citronellol is DSM 50332 for 21 days. Analytical grade samples were used for the test. Test performed in 0.5 liter of agar bottle plates by using the pure culture of microorganism providing proper temperature and PH. Culture were inoculated in dark having the mesophilic temperature. Growth inhibition of Pseudomonas citronelloliswas observed at 2m M of concentration of menthone for minimum 10 days to 3 weeks. Analytical grade samples were used for the test. Thus after the exposure with chemical no inhibition was observed. On the basis of no growth inhibition of microorganisms Pseudomonas citronellolis due to the menthone for 10 days to 3 weeks, the NOEC was 308 mg/l (2mM).
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