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EC number: 212-220-6 | CAS number: 770-12-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1997-05-30 to 1997-07-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 1996-02-22
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Phenyl phosphorodichloridate
- EC Number:
- 212-220-6
- EC Name:
- Phenyl phosphorodichloridate
- Cas Number:
- 770-12-7
- Molecular formula:
- C6H5Cl2O2P
- IUPAC Name:
- phenyl dichlorophosphate
- Test material form:
- liquid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- 10% liver S9 in standard co-factor
- Test concentrations with justification for top dose:
- preliminary test: 0, 50, 150, 1500 and 5000 µg/plate
Main test: 0, 50, 150, 500, 1500 and 5000 µg/plate.
5000 µg/plate is the maximum recommended dose (No visible reduction in the growth of the bacterial lawn. - Vehicle / solvent:
- water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- N-ethyl-N-nitro-N-nitrosoguanidine
- mitomycin C
- other: 2-Aminoanthracene and 1,8-dihydroxyanthroquinone (danthron) in the experiment with S9-mix
- Details on test system and experimental conditions:
- See any other information on materials and methods incl.tables
- Evaluation criteria:
- See any other information on materials and methods incl.tables
- Statistics:
- Dunnett's method of linear regression
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Preliminary toxicity study
The mean number of revertant colonies for the toxicity assay report in the table below
Strain |
Dose (µg/plate) |
|||||
0 |
50 |
150 |
500 |
1500 |
5000 |
|
TA100 |
114 |
123 |
107 |
113 |
108 |
98 |
Experiment 1 without metabolic activation
With or witjout metabolic activation |
Concentration (µg/plate) |
Number of revertant (number of colonies per plate) |
|||||||||
Base-pair substitution type |
Frameshift type |
||||||||||
TA100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
|||||||
- |
0 |
83 |
|
14 |
|
215 |
|
20 |
|
9 |
|
|
(78) |
|
(17) |
|
(224) |
|
|
|
(9) |
||
74 |
|
18 |
|
220 |
|
10 |
|
12 |
|
||
|
4.5 |
|
3.1 |
|
12.1 |
|
|
|
3.0 |
||
78 |
|
20 |
|
238 |
|
13 |
|
6 |
|
||
- |
50 |
79 |
|
13 |
|
220 |
|
22 |
|
10 |
|
|
(78) |
|
(15) |
|
(224) |
|
(14) |
|
(11) |
||
72 |
|
19 |
|
211 |
|
23 |
|
7 |
|
||
|
5.1 |
|
3.5 |
|
14.8 |
|
5.1 |
|
4.6 |
||
82 |
|
13 |
|
240 |
|
19 |
|
16 |
|
||
- |
150 |
79 |
|
15 |
|
230 |
|
12 |
|
8 |
|
|
(77) |
|
(16) |
|
(223) |
|
(21) |
|
-10) |
||
71 |
|
18 |
|
226 |
|
14 |
|
12 |
|
||
|
4.9 |
|
1.5 |
|
8.9 |
|
2.1 |
|
2.1 |
||
80 |
|
16 |
|
213 |
|
20 |
|
11 |
|
||
- |
500 |
71 |
|
20 |
|
249 |
|
16 |
|
10 |
|
|
(75) |
|
(17) |
|
(230) |
|
(15) |
|
(10) |
||
82 |
|
18 |
|
232 |
|
23 |
|
14 |
|
||
|
5.9 |
|
3.1 |
|
19.6 |
|
4.2 |
|
4 |
||
73 |
|
14 |
|
210 |
|
17 |
|
6 |
|
||
- |
1500 |
81 |
|
11 |
|
234 |
|
13 |
|
7 |
|
|
(76) |
|
(13) |
|
(226) |
|
(19) |
|
(10) |
||
75 |
|
17 |
|
220 |
|
11 |
|
13 |
|
||
|
5.0 |
|
3.8 |
|
7.4 |
|
3.8 |
|
3.1 |
||
71 |
|
10 |
|
223 |
|
21 |
|
11 |
|
||
- |
5000 |
74 |
|
13 |
|
236 |
|
10 |
|
16 |
|
|
(77) |
|
(14) |
|
(226) |
|
(15) |
|
(12) |
||
86 |
|
17 |
|
229 |
|
15 |
|
11 |
|
||
|
7.6 |
|
3.1 |
|
11.8 |
|
4.5 |
|
3.2 |
||
72 |
|
11 |
|
213 |
|
19 |
|
10 |
|
||
Positive control |
Name |
ENNG |
ENNG |
MMC |
4NQO |
9AA |
|||||
S9-Mix |
Concentration (µg/plate) |
3 |
5 |
0.5 |
0.2 |
80 |
|||||
- |
Number of colonies per plate |
890 |
|
791 |
|
529 |
|
247 |
|
874 |
|
|
(863) |
|
(825) |
|
(576) |
|
(218) |
|
(800) |
||
797 |
|
882 |
|
618 |
|
199 |
|
802 |
|
||
|
57.1 |
|
49.9 |
|
44.8 |
|
25.5 |
|
75.0 |
||
901 |
|
801 |
|
582 |
|
208 |
|
724 |
|
ENNG: N -ethy 1- N' -nitro- N -n itrosoguanidine
MMC: Mitomycin C
4NQO:4-nitroquinoline-1-oxide
9AA: 9-aminoacridine
Experiment 1 with metabolic activation
With or witjout metabolic activation |
Concentration (µg/plate) |
Number of revertant (number of colonies per plate) |
|||||||||
Base-pair substitution type |
Frameshift type |
||||||||||
TA100 |
TA1535 |
TA102 |
TA98 |
TA1537 |
|||||||
+ |
0 |
86 |
|
11 |
|
216 |
|
10 |
|
7 |
|
|
(80) |
|
(14) |
|
(227) |
|
(15) |
|
(11) |
||
73 |
|
13 |
|
235 |
|
21 |
|
11 |
|
||
|
6.7 |
|
3.1 |
|
9.7 |
|
5.6 |
|
3.5 |
||
82 |
|
17 |
|
229 |
|
14 |
|
14 |
|
||
+ |
50 |
90 |
|
21 |
|
242 |
|
14 |
|
10 |
|
|
(84) |
|
(17) |
|
(233) |
|
(18) |
|
(12) |
||
89 |
|
19 |
|
220 |
|
23 |
|
10 |
|
||
|
9.5 |
|
5.3 |
|
1.4 |
|
5.0 |
|
2.9 |
||
73 |
|
11 |
|
236 |
|
13 |
|
15 |
|
||
+ |
150 |
91 |
|
15 |
|
221 |
|
15 |
|
12 |
|
|
(78) |
|
(16) |
|
(217) |
|
(18) |
|
(10) |
||
71 |
|
13 |
|
221 |
|
23 |
|
9 |
|
||
|
11.0 |
|
3.6 |
|
6.4 |
|
4.6 |
|
2.1 |
||
73 |
|
20 |
|
210 |
|
15 |
|
8 |
|
||
+ |
500 |
79 |
|
10 |
|
231 |
|
17 |
|
13 |
|
|
(80) |
|
(12) |
|
(226) |
|
(16) |
|
(8) |
||
85 |
|
13 |
|
217 |
|
12 |
|
2 |
|
||
|
4.2 |
|
2.1 |
|
7.6 |
|
3.2 |
|
2.1 |
||
77 |
|
14 |
|
229 |
|
18 |
|
9 |
|
||
+ |
1500 |
80 |
|
10 |
|
226 |
|
15 |
|
6 |
|
|
(77) |
|
(13) |
|
(219) |
|
(16) |
|
(6) |
||
73 |
|
11 |
|
210 |
|
21 |
|
7 |
|
||
|
3.6 |
|
4.4 |
|
8.3 |
|
4.6 |
|
0.6 |
||
78 |
|
18 |
|
222 |
|
12 |
|
6 |
|
||
+ |
5000 |
76 |
|
19 |
|
222 |
|
12 |
|
7 |
|
|
(78) |
|
(18) |
|
(221) |
|
(15) |
|
(8) |
||
80 |
|
20 |
|
215 |
|
18 |
|
11 |
|
||
|
2.1 |
|
2.6 |
|
5.6 |
|
3.1 |
|
2.6 |
||
79 |
|
15 |
|
226 |
|
14 |
|
6 |
|
||
Positive control |
Name |
2AA |
2AA |
DANTHRON |
2AA |
2AA |
|||||
S9-Mix |
Concentration (µg/plate) |
1 |
2 |
10 |
0.5 |
2 |
|||||
+ |
Number of colonies per plate |
1045 |
|
325 |
|
510 |
|
386 |
|
321 |
|
|
(1046) |
|
(302) |
|
(543) |
|
(312) |
|
(329) |
||
1121 |
|
288 |
|
503 |
|
256 |
|
350 |
|
||
|
74.0 |
|
19.9 |
|
62.7 |
|
65.9 |
|
18.7 |
||
973 |
|
294 |
|
615 |
|
292 |
|
315 |
|
2AA: 2-aminoanthracene
DANTHRON: 1,8-dihydroxyanthraquinone
Applicant's summary and conclusion
- Conclusions:
- The phenyl dichlorophosphate was considered to be non-mutagenic under the conditions of this test
- Executive summary:
Salmonella typhimurium strains T A 1535, T A 1537, T A 102, T A98 and T A 100 were treated with the test material using the Ames plate incorporation method at five dose levels, in triplicate, both with and without the addition of a rat liver homogenate metabolising system (10% liver S9 in standard co-factors). The dose range was determined in a preliminary toxicity assay and was 50 to 5000 Jig/plate in the first
experiment. The experiment was repeated on a separate day using the same dose range as experiment 1, fresh cultures of the bacterial strains and fresh test material formulations. The method used conforms with the OECD Guidelines for the Testing of Chemicals, Protocol No. 471 and also with Method B14 in Commission Directive 92/69/EEC.
The vehicle (sterile distilled water) control plates produced counts of revertant colonies within the normal range.
All of the positive control chemicals used in the test induced marked increases in the frequency of revertant colonies, both with and without the metabolising system.
The test material caused no visible reduction in the growth of the bacterial lawn at any of the dose levels to any of the strains of Salmonella tested. The test material was, therefore, tested up to the maximum recommended dose of 5000 µg/plate.
No significant increase in the frequency of revertant colonies was recorded for any of the bacterial strains with any dose of the test material, either with or without metabolic activation. The test material was considered to be non-mutagenic under the conditions of this test.
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