Ethylenediamine-N,N'-di(acetic acid)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

Not reported

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Summary information only, very limited information is available for the interpretation of the results.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1962

Report date:

1962

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

This study followed internal Dow procedures.

GLP compliance:

no

Test type:

other: conclusion from other data

Test material

Test material information

Constituent 1

Reference substance name:

Trisodium 2-(carboxylatomethyl(2-hydroxyethyl)amino)ethyliminodi(acetate)

EC Number:

205-381-9

EC Name:

Trisodium 2-(carboxylatomethyl(2-hydroxyethyl)amino)ethyliminodi(acetate)

Cas Number:

139-89-9

Molecular formula:

C10H18N2O7.3Na

IUPAC Name:

trisodium [{2-[bis(carboxylatomethyl)amino]ethyl}(2-hydroxyethyl)amino]acetate

Details on test material:

- Name of test material (as cited in study report): Versenol 120, Glycine: N-(carboxy methyl)-N'-(2-hydroxyethyl)- N,N'-ethylenedi-trisodium salt
- Molecular formula (if other than submission substance): C10H15N2Na3O7
- Molecular weight (if other than submission substance): 344.20
- Physical state: liquid
- Analytical purity: 41.2%
- Color: yellow
- All other template details: Not reported

Test animals

Species:

rabbit

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

Conclusion is based on the skin contact test

Administration / exposure

Type of coverage:

not specified

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: ear, belly
- Number of exposures: 9 to intact ear and belly, 1 to abraded belly
- All other template details: Not reported

Duration of exposure:

Up to 21 days

Doses:

Not reported

No. of animals per sex per dose:

Not reported

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: up to 21 days
- Frequency of observations and weighing: not reported
- Necropsy of survivors performed: yes/
- Other examinations performed: not reported

Statistics:

Not reported

Results and discussion

Preliminary study:

There is no indication from the skin irritation tests conducted that this material is absorbed through the skin in toxic amounts.

Mortality:

Not reported

Clinical signs:

other: Not reported

Gross pathology:

See Table 1

Other findings:

Not reported

Any other information on results incl. tables

Table 1. Summary of skin contact results

Material	Condition of skin	Site	Number of applications	Responses
Undiluted	Intact	Ear	9	Very slight hyperemia.
Undiluted	Intact	Belly	9	Very slight hyperemia.
Undiluted	Abraded	Belly	1	Moderate hyperemia, edema and necrosis after first application, moderate scab formed on the fourth day of exposure, skin healed in 21 days with moderate scar.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

There is no indication from the skin irritation tests conducted that this material is absorbed through the skin in toxic amounts.