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Diss Factsheets
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EC number: 216-372-4 | CAS number: 1569-01-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Dec 1995 to Aug 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-study according to OECD guideline 471.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5265 (The Salmonella typhimurium Bacterial Reverse Mutation Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-propoxypropan-2-ol
- EC Number:
- 216-372-4
- EC Name:
- 1-propoxypropan-2-ol
- Cas Number:
- 1569-01-3
- Molecular formula:
- C6H14O2
- IUPAC Name:
- 1-propoxypropan-2-ol
- Details on test material:
- - Name of test material (as cited in study report): Propylene glycol n-propyl ether (DOWANOL PnP)
- Physical state: clear colorless liquid
- Lot/batch No.: 950720
Constituent 1
Method
- Target gene:
- Induction of reverse mutations at the histidine locus of S. typhimurium and the tryptophan locus in E. coli.
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- not applicable
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- not applicable
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 Mix
- Test concentrations with justification for top dose:
- 0, 100, 333, 1000, 3330 and 5000 µg/plate
- Vehicle / solvent:
- Water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (TA98, TA100, TA1535, TA1537, WP2uvrA/+S9); 2-nitrofluorene (TA98/-S9); sodium azide (TA100, TA1535/-S9); ICR-191 (TA1537/-S9); 4-nitroquinoline-N-oxide (WP2uvrA/-S9)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 min
- Incubation period: 48 hours
NUMBER OF REPLICATIONS: 3 replicates per dose / 2 independent experiments
DETERMINATION OF CYTOTOXICITY
- Method: bacterial background lawn evaluation (decrease in number of revertant colonies
- no cytotoxicity observed
- Evaluation criteria:
- For a test article to be considered positive, it had to produce at least a 3-fold increase in the mean revertants per plate of at least one tester strain over the mean revertants per plate of the appropriate vehicle control. This 3-fold or greater increase in the mean number of revertants per plate had to be observed at more than one dose and had to be accompanied by a dose response to increasing concentrations of the test article. In addition, the observed dose-responsive increase had to be shown to be reproducible. An observed response which did not meet all three of the above criteria (magnitude, dose-responsiveness, reproducibility) was not evaluated as positive.
- Statistics:
- None
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The results of the Salmonella - Escherichia coli/Mammalian-Microsome Reverse Mutation Assay, Preincubation Method, with a Confirmatory Assay, indicate that, under the conditions of this study, in both an initial and confirmatory assay, test article, Propylene glycol n-propyl ether, did not cause a positive increase in the number of revertants per plate of any of the tester strains either in the presence or absence of microsomal enzymes prepared from Aroclor-induced liver (S9). - Executive summary:
Propylene glycol n-propyl ether (DOWANOL PnP), was evaluated in the Salmonella-Escherichia coli/mammalian-microsome bacterial mutagenicity assay using a pre-incubation modification of the standard assay. The test was conducted in the presence and absence of an externally supplied metabolic activation system (S-9) using Salmonella typhimurium tester strains TA98, TA100, TA1535, and TA1537 and Escherichia coli tester strain WP2ucrA. The concentrations of the test material assay ranged from 100 to 5000 µg/plate. The test material did not induce a positive increase in the number of revertant colonies in any of the tester strains either in the presence or absence of the external metabolic activation system. The results were confirmed in an independent assay. Hence, propylene glycol n-propyl ether (DOWANOL PnP) was classified as negative in this bacterial mutagenicity test under experimental conditions used.
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