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EC number: 204-844-2 | CAS number: 127-47-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-09-03 to 1993-01-07
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test carried out under GLP and according to guideline 301B. Some deviations of guideline, but these are not essential for reliability.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- volume of test solution was reduced from 3 to 1.5 L. The formed CO2 was absorbed with NaOH and determined on a carbon analyzer. Due to poor solubility of the substance in water, an emulsifier was used.
- Principles of method if other than guideline:
- not relevant
- GLP compliance:
- yes
Test material
- Reference substance name:
- Retinyl acetate
- EC Number:
- 204-844-2
- EC Name:
- Retinyl acetate
- Cas Number:
- 127-47-9
- Molecular formula:
- C22H32O2
- IUPAC Name:
- (2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohex-1-en-1-yl)nona-2,4,6,8-tetraen-1-yl acetate
- Details on test material:
- - Name of test material (as cited in study report): Vitamin A-Acetat
- Physical state: yellow solid
- Radiochemical purity (if radiolabelling): not relevant
- Storage condition of test material: room temperature, in dark
- Other:
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not relevant
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sewage treatment plant CH-4153 Reinach dd
1992-08-07
- pH after collection was 7.6 - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 10.3 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20.9 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 1200 ml mineral solution with the inoculum and 15 ml with solubilising agent and test substance
- Solubilising agent (type and concentration if used): 15 ml polyoxyehtylen-Sorbitan-Monooleate (TWEEN 80), 300 mg/L water
- Test temperature: 22 ± 2 degrees C
- Aeration of dilution water:ca 25 ml air/min purified from carbon dioxide
- Continuous darkness: yes, test carried out in dark brown flasks
TEST SYSTEM
- Culturing apparatus: test vessels containing 1.5 L of medium
- Number of culture flasks/concentration: 2 for the blank and the reference substance, one for each of the two test substance concentrations
- Measuring equipment: Carbon analyzer
SAMPLING
- Sampling frequency: every 3-4 days until 28 days
- Sampling method: CO2 traps filled with 200 ml 0.05 N NaOH
CONTROL AND BLANK SYSTEM
- Inoculum blank: water with inoculum containing 15 ml of TWEEN 80 solution
- Other: Inoculum control with aniline as reference substance
Reference substance
- Reference substance:
- aniline
- Remarks:
- 20 mg/L
Results and discussion
- Preliminary study:
- no data
- Test performance:
- not relevant: no unusual observations
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 18.5
- Sampling time:
- 28 d
- Remarks on result:
- other: 10.3 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 42.2
- Sampling time:
- 28 d
- Remarks on result:
- other: 20.9 mg/L
- Details on results:
- calculated biodegradation after 28 days:
inoculum with 10.3 mg/L: 15%
inoculum with 20.9 mg/L: 33%
BOD5 / COD results
- Results with reference substance:
- CO2 evolution after 28 days: 88.9 and 84.2 mg,
Calculated biodegradation after 28 days: 91 and 85% (mean 88&%), meets 10d-window
Any other information on results incl. tables
measured values on 27th day are not reliable due to instrumental defect and are not taken for calculations
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The validity criteria (reproducibility within 5%) can only be checked in reference test. Is OK.
- Interpretation of results:
- other: not readily biodegradable under the conditions of the test
- Conclusions:
- Biodegradation curve shows steady increase of degradation indicating diffusion limitation due to poor water solubility.
Biodegradation after 28 days is 15 and 33% for concentration of 10.3 and 20.9 mg/L respectively. Substance is not readily biodegradable under the conditions of this test. - Executive summary:
Determination of the biodegradability by measurement of the CO2 formation in % of ThCO2 (Theoretical CO2) calculated from the ThOC (Theoretical organic carbon) according to OECD guideline 301B. In comparison to guideline, the volume of test solution was reduced from 3 to 1.5 L, the formed CO2 was absorbed with NaOH and determined on a carbon analyzer. Due to poor solubility of the substance in water, an emulsifier was used. An inoculum of a sewage treatment plant was used. The biodegradation calculated as % of measured amount of CO2 over the theory after 28 days was 15% for the 10.3 mg test substance/L and 33% for the 20.9 mg test substance/L. The biodegradation curve shows a steady increase of degradation indicating diffusion limitation due to poor water solubility. The validity criteria were met. The test substance is not readily biodegradable under the conditions of this test.
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