Ethyl trans-2,2,6-trimethylcyclohexanecarboxylate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 April 1993 to 23 April 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Method of Preparation
Preliminary solution in 1% v/v Tween 80-ethanol. Fresh solvent stock solutions of 0.50, 0.90, 1.60, 2.80 and 5.00 g test material/ 25 mL solvent were prepared daily for dosing by the dynamic, continuous flow dosing apparatus.
Stability of test concentrations
Ensured by continuous renewal of the test media and verified by chemical analysis of the solvent control and all surviving test groups at 0, 24, 48, 72 and 96 hours. Analysis of the solvent stock solutions used to prepare the test series at -24, 0, 24, 48 and 72 hours was also performed. Data relating to the identity, purity and stability of the supplied test material are the responsibility of the Sponsor.

Vehicle:

yes

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Name: Rainbow trout (Oncorhynchus mykiss).
Source: Parkwood Trout Farm, Wigmore, Kent, U.K.
Acclimatisation: Stock held since 17 February 1993 and acclimatised to test conditions from 12 - 19 April 1993.The stock fish were maintained in a glass fibre tank with a "single pass" water renewal system. The lighting was controlled to give a 16 hours light and 8 hours darkness cycle.
Feeding: Commercial trout pellets daily. Discontinued 24h prior to test.
Mortalities: <1% in the 7 days prior to the start of the test.
Medication: None
Mean standard length: 4.7 cm (SD= 0.3cm)
Mean weight: 0.93g (SD=0.26g)

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Total hardness approximately 50 mg/L as CaCO3

Test temperature:

Temperature: 14°C

pH:

7.3-7.5

Dissolved oxygen:

Dissolved Oxygen: >= 9 mg 02/ L

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal test concentrations : 2.0, 3.6, 6.4, 11.2 and 20 mg/l. These test concentrations were assigned following preliminary range-finding studies, where 3 fish were exposed to each of the following concentrations for a period of 96 hours: 0.10, 1.0, 10 and 20 mg/l. The results showed no mortalities at the concentrations of 0.10, 1.0 and 10 mg/l. However mortalities were observed at 20 mg/l, thereby indicating the choice of test concentrations employed.
Measured test concentrations:
At nominal of 2 mg/l: 1.570 mg/l (79%)
At nominal of 3.6 mg/l: 3.279 mg/l (91%)
At nominal of 6.4 mg/l: 6.116 mg/l (96%)
At nominal of 11.2 mg/l: 9.836 mg/l (88%)
At nominal of 20 mg/l: 21.069 mg/l (105%)

Details on test conditions:

Test vessels : Glass aquaria holding 20 L of test media.
Experimental design : 5 test concentrations plus 1 control and 1 solvent control (including 100 ul/ L auxiliary sol vent) .10 animals per test concentration.
Method of initiation : Fish were placed, at random, in prepared test media.
Loading : 0.47 g bodyweight / llitre. (static volume), 0.11 g bodyweight/ li tre (volume 24 hours) and 4.25 tank volumes per day.
Photoperiod : 16h light:8h dark.
Aeration : The diluent supply only was aerated.
Medium renewal : Continuous at 59 ml/min. .The diluent supply was pumped at 59 ml/min by a Watson Marlow peristaltic pump with solvent stock solutions dosed by a Braun Perfusor Secura syringe pump at a rate of 0.3553 ml/ hr. The dosing apparatus was set at approximately 24 hours prior to the start of the study to allow equilibration of test concentrations.
Environmental measurements : pH, dissolved oxygen concentration and temperature were recorded daily throughout the study.

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

7.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

7.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

7.1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1.6 mg/L

Details on results:

See attached document

Validity criteria fulfilled:

yes

Conclusions:

NOEC (96h)= 1.6 mg/ L

Executive summary:

TEST MATERIAL ET-344 -SP

TEST SPECIES Rainbow trout (Oncorhynchus mykiss)

TEST TYPE Acute toxicity . 96 h LC50.

TEST CONDITIONS Dynamic test conditions according to OECD Guideline No. 203 referenced as Method C. l of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/548/EEC).

RESULTS
Duration	Endpoint	Effect conc.	Nominal/Measured	Conc. based on	Basis for effect	Remarks (e.g. 95% CL)
24 h	LC50	7.8 mg/L	meas. (arithm. mean)	test mat.	mortality
48 h	LC50	7.8 mg/L	meas. (arithm. mean)	test mat.	mortality
72 h	LC50	7.1 mg/L	meas. (arithm. mean)	test mat.	mortality
96 h	LC50	6 mg/L	meas. (arithm. mean)	test mat.	mortality
96 h	NOEC	1.6 mg/L

Description of key information

TEST MATERIAL ET-344 -SP

TEST SPECIES Rainbow trout (Oncorhynchus mykiss)

TEST TYPE Acute toxicity . 96 h LC50.

TEST CONDITIONS Dynamic test conditions according to OECD Guideline No. 203 referenced as Method C. l of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/548/EEC).

RESULTS
Duration	Endpoint	Effect conc.	Nominal/Measured	Conc. based on	Basis for effect	Remarks (e.g. 95% CL)
24 h	LC50	7.8 mg/L	meas. (arithm. mean)	test mat.	mortality
48 h	LC50	7.8 mg/L	meas. (arithm. mean)	test mat.	mortality
72 h	LC50	7.1 mg/L	meas. (arithm. mean)	test mat.	mortality
96 h	LC50	6 mg/L	meas. (arithm. mean)	test mat.	mortality
96 h	NOEC	1.6 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

6 mg/L