Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-209-2 | CAS number: 135-65-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-hydroxy-3'-nitro-2-naphthanilide
- EC Number:
- 205-209-2
- EC Name:
- 3-hydroxy-3'-nitro-2-naphthanilide
- Cas Number:
- 135-65-9
- Molecular formula:
- C17H12N2O4
- IUPAC Name:
- 3-hydroxy-N-(3-nitrophenyl)-2-naphthamide
- Test material form:
- solid
- Details on test material:
- purity: 99.7%
Product number HKAG 155
Constituent 1
- Specific details on test material used for the study:
- purity: 99.7%
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was weighed into a beaker. Water for dilution was added and the mixture transferred quantitatively into a 5 liter glass bottle. The pH was adjusted with 10 molar NaOH to the pH of 10.0 to facilitate the dissolvation of the test substance in the test water. The chamber contents were stirred for approximately 24 hours with a magnetic-stirrer. Then the pH was adjusted to the pH of the test water with conc. HCI.
The suspension was filtered off with a cellulose filter to remove undissolved parts of the test substance.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish; Brachydanio rerio (Hamilton-Buchanan)
- Age at study initiation: approx. 5 months
- Length at study initiation: 2.5 - 3.0 cm
-Origin: Corporate Toxicology, Hoechst AG - Hoechst Marion Roussel
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation: Tetra Min, twice a day ad libitum
FEEDING DURING TEST: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- 2.3 - 2.5 mmol Ca(2+) and Mg(2+)
- Test temperature:
- 22 +-1 °C
- pH:
- 7.9 - 8-1
- Dissolved oxygen:
- 7.5 - 8.8 mg/L
- Nominal and measured concentrations:
- nominal : 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessel 20 cm x 15cm x 20 cm
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
OTHER TEST CONDITIONS
- Photoperiod: 06.00 am to 06.00 pm
- Light intensity: approx. 700 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Test concentrations: 1000 mg/L nominal - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this test the LC5O of Naphtol AS-BS after 96 hours was > 1000 mg/L (based on loading) and is above the solubility limit in the test water.
- Executive summary:
Naphtol AS-BS was tested in zebra fish (Brachydanio rerio) over 96 hours in a static system. A limit test with a loading of 1 000 mg/L Naphtol AS-BS and a negative control (0 mg/L) was conducted.
After adding the substance the pH was adjusted with 10 molar NaOH to the pH of 10.0 to facilitate the dissolvation of the test substance in the test water. The chamber contents were stirred for approximately 24 hours with a magnetic-stirrer. Then the pH was adjusted to the pH of the test water with conc. HCI. The suspension was filtered off with a cellulose filter to remove undissolved parts of the test substance.
The tested concentration was present as light yellow solution. The test batch was homogeneous. No particulate matter was observed. The substance concentration was determined in the 1000 mg/L group. Due to the very low solubility of the test substance in water (water solubility = 0.35 mg/L), the values determined were in a range of only 0.60-0.68 mg/L (mean 0.64 mg/L). All lethality values given in this report are based on loading rates.
No lethality was observed up to the solubility limit of Naphtol AS-BS in the test water under the conditions of this test. The 1 000 mg/L group exhibited symptoms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.