Methyl pyruvate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented and conducted according to OECD guideline.

Data source

Reference

Reference Type:

publication

Title:

Discrepancies in the acute versus chronic toxicity of compounds with a designated narcotic mechanism

Author:

Dom, N. et al.

Year:

2012

Bibliographic source:

Chemosphere, 87: 742-749

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

Molecular formula:

CH4O

IUPAC Name:

methanol

Details on test material:

- Name of test substance (as cited in study report): methanol
- Supplier of test substance: VWR (Belgium)
- Purity: 99.8 %

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

CULTURING
- Culturing: single clone Daphnia magna (clone K6) cultures were held in 1 L glass recipients filled with aerated and biologically filtered tap water, each containing 20 - 25 individuals.
- Conditions: the temperature in the temperature and light controlled chamber (Type WT150'/+5DU-WB, Weiss Technik, Liedekerke, Belgium) was maintained at 20 ± 1 °C with a photoperiod of 14 h light/10 h dark throughout culturing and exposure experiments.
- Water renewal: three times weekly
- Feeding: the daphnids were fed a mixture of P. subcapitata and Chlamydomonas reinhardtii in a 3/1 ratio (4 x 10E5 cells/ml)

- Age of daphnids at test initiation: < 24 h

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96

Test conditions

Hardness:

initial water hardness: 250 mg CaCO3

Test temperature:

20 ± 1 °C

pH:

initial pH: 7.8-8.2

Nominal and measured concentrations:

- Nominal concentrations (linear 1/2 dilution series): 1 - 32 g/L

Details on test conditions:

TEST SYSTEM
- Test vessels: glass recipients
- Test volume: 50 ml
- No of animals/replicate: 10
- No of replicates/concentration: 3
- Test medium: reconsituted water
- Test media renewal: every 24 h

TEST MEDIUM
- Medium: reconstituted water

TEST CONDITIONS
- Feeding: daphnids were fed the same algae mixture as during culturing
- Photoperiod: 14 h light/ 10 h dark

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

18 260 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility