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EC number: 242-644-7 | CAS number: 18880-36-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 August - 26 August 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP - Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Methods for Determination of Toxocity , Annex to Directive 92/69/EEC , Method B14 , Other effects - Mutagenicity : Samonella typhimurium - Reverse Mutation Assay
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: conforms to the USA, EPA (TSCA) guidelines
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- (The Department of Health of the Government of the United Kingdom)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Sodium 3-[[(dimethylamino)thioxomethyl]thio]propanesulphonate
- EC Number:
- 242-644-7
- EC Name:
- Sodium 3-[[(dimethylamino)thioxomethyl]thio]propanesulphonate
- Cas Number:
- 18880-36-9
- Molecular formula:
- C6H13NO3S3.Na
- IUPAC Name:
- sodium 3-[(dimethylcarbamothioyl)sulfanyl]propane-1-sulfonate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): DPS ; 1-Propanesulfonic acid , 3-[[(dimethylamino)thioxomethyl]thio] , sodium salt
- Substance type: white powder
- Analytical purity: 90%
- Lot/batch No.: 92002
- Storage condition of test material: room temperature over silica gel
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver homogenate metabolising system (10% liver S9 in standard co-factors)
- Test concentrations with justification for top dose:
- Preliminary toxicity study : 0 , 312.5 , 625 , 1250 , 2500 and 5000 µg/plate
First (Range-Finding study) and second (Main study) experiment : 0 , 8 , 40 , 200 , 1000 and 5000 µg/plate with and without metabolic activation - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: sterile distiiled water
- Justification for choice of solvent/vehicle: the test material was soluble in water up to 50 mg/mL
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- N-ethyl-N-nitro-N-nitrosoguanidine
- other: 2-Aminoanthracene
- Remarks:
- See -Any other information on materials and methods incl. tables- for details
- Details on test system and experimental conditions:
- METHOD OF APPLICATION : in agar (plate incorporation)
DURATION
- Exposure duration: 48 h
NUMBER OF REPLICATIONS : one preliminary toxicity test in duplicate, mutation assay in triplicate, two independent experiments
DETERMINATION OF CYTOTOXICITY
- Method: Assessment for numbers of revertant colonies and examination for effects on the growth of the bacterial background lawn . - Evaluation criteria:
- For a substance to be considered positive in this test system , it should have induced a dose-related and statistically significant increase in mutation rate (of at least twice the spontaneous reversion rate) in one or more strains of bacteria in the presence and/or absence of the S9 microsomal enzymes in both experiments at sub-toxic dose levels .
To be considered negative , the number of induced revertants compared to spontaneous revertants should be less than twofold at each dose level employed , the intervals of which should be between 2 and 5 fold extend to the limits imposed by toxicity or solubility or up to the maximum recommended dose of 5000 µg/plate . In this case the limiting factor was the maximum recommended dose . - Statistics:
- A statistical analysis of the data was not required to determine the result of the test.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The results of the checks for characteristics , viability and spontaneous reversion rate for each tester strain were all found to be satisfactory .
Any other information on results incl. tables
Table 1 : Range-finding study
Mean number of revertant colonies per plate (average of 3 plates) | ||||||
With or without S9 -Mix | Test substance concentration (µg/plate) | Base-pair substitution type | Frameshift type | |||
TA 100 | TA 1535 | WP2uvrA- | TA 98 | TA 1537 | ||
- | 0 | 117 | 22 | 38 | 24 | 13 |
- | 8 | 120 | 16 | 35 | 20 | 15 |
- | 40 | 114 | 23 | 42 | 25 | 13 |
- | 200 | 117 | 24 | 42 | 22 | 14 |
- | 1000 | 113 | 22 | 32 | 17 | 13 |
- | 5000 | 124 | 24 | 35 | 21 | 13 |
Positive controls , -S9 | Name | ENNG | ENNG | ENNG | 4NQO | 9AA |
Concentrations (µg/plate) | 3 | 5 | 2 | 0,2 | 80 | |
- | Mean No. of colonies/plate (average of 3) | 505 | 175 | 895 | 188 | 338 |
+ | 0 | 154 | 22 | 36 | 35 | 14 |
+ | 8 | 147 | 22 | 37 | 26 | 12 |
+ | 40 | 150 | 28 | 36 | 34 | 12 |
+ | 200 | 132 | 21 | 32 | 27 | 10 |
+ | 1000 | 135 | 22 | 32 | 26 | 14 |
+ | 5000 | 150 | 20 | 32 | 28 | 10 |
Positive controls , +S9 | Name | 2AA | 2AA | 2AA | 2AA | 2AA |
Concentration (µg/plate) | 1 | 2 | 10 | 0,5 | 2 | |
+ | Mean No. of colonies)plate (average of 3) | 608 | 214 | 168 | 497 | 202 |
ENNG = N-ethyl-N´-nitro-N-nitrosoguanidine
4NQO = 4-nitroquinoline-1 -oxide
9AA = 9-aminoacridine
2AA = 2-aminoanthracene
Table 2 : Main study
Mean number of revertant colonies per plate (average of 3 plates) | ||||||
With or without S9 -Mix | Test substance concentration (µg/plate) | Base-pair substitution type | Frameshift type | |||
TA 100 | TA 1535 | WP2uvrA- | TA 98 | TA 1537 | ||
- | 0 | 106 | 21 | 39 | 30 | 13 |
- | 312.5 | 113 | 17 | 30 | 22 | 13 |
- | 625 | 102 | 20 | 32 | 21 | 14 |
- | 1250 | 103 | 23 | 31 | 24 | 15 |
- | 2500 | 107 | 22 | 35 | 19 | 13 |
- | 5000 | 107 | 28 | 36 | 22 | 13 |
Positive controls , -S9 | Name | ENNG | ENNG | ENNG | 4NQO | 9AA |
Concentrations (µg/plate) | 3 | 5 | 2 | 0,2 | 80 | |
- | Mean No. of colonies/plate (average of 3) | 410 | 161 | 588 | 128 | 456 |
+ | 0 | 114 | 12 | 36 | 38 | 14 |
+ | 312.5 | 124 | 13 | 41 | 25 | 15 |
+ | 625 | 113 | 11 | 39 | 30 | 15 |
+ | 1250 | 109 | 14 | 39 | 35 | 12 |
+ | 2500 | 120 | 10 | 33 | 28 | 15 |
+ | 5000 | 119 | 9 | 39 | 31 | 20 |
Positive controls , +S9 | Name | 2AA | 2AA | 2AA | 2AA | 2AA |
Concentration (µg/plate) | 1 | 2 | 10 | 0,5 | 2 | |
+ | Mean No. of colonies)plate (average of 3) | 831 | 139 | 185 | 582 | 234 |
ENNG = N-ethyl-N´-nitro-N-nitrosoguanidine
4NQO = 4-nitroquinoline-1 -oxide
9AA = 9-aminoacridine
2AA = 2-aminoanthracene
Applicant's summary and conclusion
- Conclusions:
- The study was performed according to the OECD TG471 without deviations and therefore considered to be of the highest quality (reliability Klimisch 1). The validity criteria of the test system are fulfilled. No significant increase in the numbers of revertant colonies was recorded for any of the bacterial strains , with any dose of DPS , either with or without metabolic activation. DPS was found to be non-mutagenic under the conditions of this test .
- Executive summary:
Salmonella tvphimurium strains TA1535, TA1537, TA98 and TA100 and Escherichia coli strain WP2uvrA-were treated with DPS by the Ames plate incorporation method at five dose levels, in triplicate both with and without the addition of a rat liver homogenate metabolising system (10% liver S9 in standard co-factors). This method conforms to the guidelines for bacterial mutagenicity testing published by the major Japanese Regulatory Authorities including MITI, MHW, MOL and MAFF. It also meets the requirements of the OECD, EEC and USA, EPA (TSCA) guidelines. The dose range was determined in a preliminary toxicity assay and was 8 to 5000 µg/plate in the first experiment. The experiment was repeated on a separate day using different cultures of the bacterial strains and fresh chemical formulations. In this case the dose range of DPS was 312.5 to 5000 µg/plate. The vehicle (sterile distilled water) control plates gave counts of revertant colonies within the normal range. All positive control chemicals produced marked increases in the numbers of revertant colonies, both with and without the metabolising system. DPS caused no visible reduction in the growth of the bacterial lawn at any dose level either with or without metabolic activation. DPS was therefore tested up to the maximum recommended dose level of 5000 µg/plate. No significant increase in the numbers of revertant colonies was recorded for any of the bacterial strains with any dose of DPS, either with or without metabolic activation. DPS was found to be non-mutagenic under the conditions of this test.
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