Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 416-730-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Link to relevant study record(s)
Description of key information
There were no studies about metabolism and kinetic of the test substance
performed. However, the available studies and properties indicate that
the test substance is not absorbed after oral or dermal application.
There is also no evidence for systemic distribution.
Key value for chemical safety assessment
- Bioaccumulation potential:
- no bioaccumulation potential
Additional information
No studies regarding toxicokinetic properties of the test substance were performed. Subsequently, assumptions on ADME were based on physico-chemical data and the available toxicity studies. Taking all information into account, experimental studies and physico-chemical properties indicate that the test substance is not absorbed after oral, respiratory or dermal application. There is also no evidence for systemic distribution.
Absorption
Absorption – oral
The test substance has three ionizable groups, namely hydroxyl group (OH) and two sulfonate groups, thus, being favorable for absorption. However, due to the high molecular weight (> 500 g/mol) gastrointestinal absorption is assumed to be very limited. Water solubility is suggested to be moderate (1.2 g/L). The Log P value of -1.43 indicates oral absorption to be unlikely as well. Furthermore, experimental studies indicate no oral absorption due to the absence of effects and the observation of discolored feces.
Absorption – respiratory
Particles of the test substance have an aerodynamic diameter of 47.5 μm, which have the potential to be inhaled (< 100 μm). Particle size distribution was determined, and particle size fractions were as follows:
< 4 μm = 4 %
< 10 μm = 10 %
< 100 μm = 83 %
Following inhalation, the particles are assumed to behave like inert dusts as due to the high molecular weight and the log P value absorption is expected to be unlikely.
As the test substance is a solid vapor pressure is not applicable. Experimental data on the toxicity after inhalation are not available for the test substance itself.
Absorption – dermal
Dry particulates are not taken up readily. In addition, the molecular weight of the test substance (520.975 g/mol) indicates that the molecule is too large to be taken up dermally. The water solubility of 1.2 g/L points to a moderate absorption. However, the log P of -1.43 suggests that the test substance is not likely to be sufficiently lipophilic to cross the stratum corneum, thus, dermal absorption is likely to be low. This assumption is confirmed by experimental data from a dermal acute toxicity study. Herein, no adverse effects were observed. Furthermore, the substance does not possess a skin irritating or corrosive potential, that would damage the skin surface and enhance skin penetration.
Distribution
Since absorption of the test substance is assumed to be very limited, distribution is not relevant. However, in the unlikely case of absorption, distribution may be limited due to molecular weight of above 500 g/mol. Diffusion through aqueous channels and pores is considered to be low.
Accumulative potential
Considering the log P value being below 3, an accumulative potential is suggested unlikely in adipose tissue. In addition, accumulation potential in bone and stratum corneum is considered unlikely as well.
As mentioned above, particles of the test substance can be inhaled. As only about 4 % of the particles are below 4 μm the amount of particles reaching the alveoli is small. In the unlikely case of respiration, the particles are likely to undergo phagocytosis by alveolar macrophages.
Metabolism
As no experimental data regarding metabolism is available, possible metabolites were identified with OASIS TIMES (version 2.27.19) and the metabolism simulator “in vivo rat 07.08”.
Bacterial or enzymatic cleavage of the azo group may lead to the formation of 3-(4-amino-3-methyl-5-oxo-4H-pyrazol-1-yl)benzenesulfonic acid (metabolite 1) and 2-amino-4-chloro-benzenesulfonic acid (metabolite 2). Concerning metabolite 1, limited information is available. However, studies with a test substance lacking the amine group at position 4 (m-(4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzenesulfonic acid, CAS 119-17-5) were performed. This analogue metabolite was without pathological findings in an acute toxicity study and did not induce mutations in an Ames test. In addition, based on results obtained from several in vitro genetic toxicity studies, it can be assumed that the test substance is not enzymatically activated (toxified) during metabolism as the parent compound showed no higher toxicity compared to the metabolic activated substance.
In conclusion, the test substance has a molecular weight greater than 500 g/mol indicating limited gastrointestinal absorption and systemic availability which is supported by the observation of yellow stained feces after repeated application and the absence of any treatment related effects. Thus, it could be demonstrated that, at least in part, the chromophore of the test substance is preserved.
Excretion
Based on experimental data and physico-chemical properties, the test substance is expected to be excreted mainly unchanged via feces. Discolored feces observed in experimental animals after oral application of the substance supports this assumption.
Hydrolysis products
The test substance will hydrolyze slowly in contact with water. No significant hydrolysis was observed at pH 4, 7 and 9 at 50°C over 5 days.
In general, the substance does not contain functional groups that are susceptible to pH-dependent hydrolysis at environmentally relevant pH.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)