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EC number: 805-580-6 | CAS number: 1431696-36-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guidline Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- benzyltrimethylazanium 2-hydroxy-2-methylpropanoate
- EC Number:
- 805-580-6
- Cas Number:
- 1431696-36-4
- Molecular formula:
- C14 H23 O3 N
- IUPAC Name:
- benzyltrimethylazanium 2-hydroxy-2-methylpropanoate
- Details on test material:
- - Identification: BTMA-HIB
- Chemical name: N,N,N-trimethyl -, 2-hydroxy- 2-methylpropanoate (1:1)
- Physical form: Solid
- Water solubility: Miscible
- Purity: >90%; treated as 100% pure
- Storage: at room temperature, under N2
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- HPLC analyses of the test substance concentrations were conducted in the
definitive test at the beginning of the test and after 24, 48 and 72 h of exposure.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- - Common name: Desmodesmus subspicatus
- Strain: SAG 86.81
- Source (laboratory, culture collection): Collection of algal cultures in University of Göttingen/Germany
- Age of inoculum (at test initiation): The axenic slope culture is yearly re-purchased. Potassium dichromate is used to control the sensitivity of the culture twice a year.
- Method of cultivation: 250 ml flask containing 100 ml of sterile OECD medium inoculated with cell material from axenic slope cultures; Continuous illumination (2000–3000 lux) from Osram Fluora L18W77 and Osram Daywhite L18W840 (Osram AG, Winterthur, Switzerland); 22 ± 2 °C, thermo-controlled room
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22 ± 2°C
- pH:
- 7.2-7.8
- Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL flasks
- Material, size, headspace, fill volume: 250 mL Erlenmeyer flasks, test volume 100 mL
- Shaking rate: continuous (appr. 130 rpm)
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: 0.5x10^4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per test group for estimating initial concentration control analsis only (replicates): 3
- No. of vessels per blank control for estimating initial concentration: 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD medium prepared according to OECD Guidleine for Testing of Chemicals, No. 201 Algal growth inhibition test, Mar 2006, Annex 5 corrected July 2011
- pH-value: The pH of the control medium should not increase by more than 1.5 units during the test. Determined in the medium batch which is used for the preparation and dilution of the stock solution
- Starting cell density: 0.50–0.85 μg/ml with respect to dry weight corresponding to about 2–5•103 cells/ml (i.e. OD680 of about 0.005 units)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: Source: continuous from Osram Fluora L 18W77 and Osram Daywhite L 18W840 (Osram AG, Winterthur, Switzerland);
- Light intensity and quality: Light intensity (target): the light intensity amounts to about 3000 lux which corresponds to about 40 μE•m-2•s-2 according to the OECD TG no. 2011; Homogeneity (target): the light intensity is maintained within ±15% from the average light intensity over the incubation area
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Observation: Cell concentrations were determined every 24 h using a spectrophotometer (680 nm wavelength). Aliquots of 5 ml were removed from each test flask under sterile conditions. Cell concentrations at time 0 h were only determined in the control vessels
- Chemical analysis: The chemical analysis of EMIM Dicyanamid was performed with a combined approach. The cation (1H-imidazolium, 1-ethyl-3-methyl-) was measured by HPLC. Additionally, EMIM Dicyanamid was measured by dissolved organic carbon (DOC) determinations. Both analysis were conducted in the definitive test at the beginning, and after 24, 48 and 72 h of exposure. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 198 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 189-207
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 49.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 43.7-55.0
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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