2,5,6-trimethylcyclohex-2-en-1-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study; scientifically acceptable, however, no details on the test substance characteristics were given.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,5,6-trimethylcyclohex-2-en-1-one

EC Number:

243-473-0

EC Name:

2,5,6-trimethylcyclohex-2-en-1-one

Cas Number:

20030-30-2

Molecular formula:

C9H14O

IUPAC Name:

2,5,6-trimethylcyclohex-2-en-1-one

Details on test material:

- Name of test material (as cited in study report): 2,3,6-Trimethylcyclohexen-1-one
- Analytical purity: about 95 %
water solubity: > 250 mg/l at 20 °C.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (250 mg/l) was prepared using a 30 min stirring period at 20 °C. Then, this test medium was serially diluted with dilution water to obtain the desired test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19 - 21 °C

pH:

7.4 - 8.1

Dissolved oxygen:

7.1 - 9.1 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
control, 3.91, 7.82, 15.6, 31.3, 62.6, 125.0 and 250.0

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 ml flat bottom glass tubes
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: charcoal filtered and dechlorinated tap water.


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, diffuse light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24, 48

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

62.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

62.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

83.83 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Moving average method (Spearman-Kaerber).

Any other information on results incl. tables

No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) 0 / 3 / 6 / 24 / 48
Control /  0 /   0 /   0 /   5 /   5
3.91    /  0 /   0 /   0 /   0 /   0
7.82    /  0 /   0 /   0 /   0 /   0
15.6    /  0 /   0 /   0 /   0 /   0
31.3    /  0 /   0 /   0 /   5 /   5
62.6    /  0 /   0 /   0 /   5 /   5
125.0   /  0 /  55 /  55 / 100 / 100
250.0   /  0 / 100 / 100 / 100 / 100

24 hour ECx values:
EC0: 62.6 mg/l
EC50: 83.83 mg/l
EC100: 125.0 mg/l