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Diss Factsheets
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EC number: 203-299-8 | CAS number: 105-45-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study meets generally accepted scientific standards, is well documented and acceptable for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Methyl acetoacetate
- EC Number:
- 203-299-8
- EC Name:
- Methyl acetoacetate
- Cas Number:
- 105-45-3
- Molecular formula:
- C5H8O3
- IUPAC Name:
- methyl 3-oxobutanoate
- Details on test material:
- - Name of test material (as cited in study report): Acetessigsaeuremethylester (methyl acetoacetate);
- Physical state: transparent liquid
- Analytical purity: ca. 98 %
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: SPF-Zucht, Firma WIGA, Sulzfeld, Germany
- Age at study initiation: no data
- Mean weight at study initiation: males: 183 g and females: 193 g
- Fasting period before study: no data
- Housing: no data
- Diet (e.g. ad libitum): Herilan MRH; H. Eggersmann KG, Rinteln, Germany, ad libitum after treatment
- Water (e.g. ad libitum): tap water, ad libitum, after treatment
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- no data
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- clean air
- Remarks:
- none
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
The test substance was converted into vapour by passing through a vaporiser at 120°C. For this purpose, a constant amount of test substance was conducted through the vaporiser by means of a continuous infusion pump (UNITA I, B. BRAUN, Melsungen). The test substance vapour was mixed to fresh air which was conducted through an inhalation chamber with a capacity of ca. 180 liters.
TEST ATMOSPHERE
- An analytical monitoring of the test substance was performed by means of GC. For this purpose, a series of air/vapour samples of 5 liters of the vapour-air mixture was collected from the inhalation chamber, close to the animals. The tested nominal concentration of 8.6 mg/l corresponded to a measured concentration ranging between 6.36 and 6.86 mg/l. Thus, a mean analytical dose of 6.6 mg/l was issued for the test.
TREATMENT
- After ca. 20 min. pre air flow, animals were put in the inhalation chamber through a positing system. - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Gas chromatography. The analytical monitoring of a series of air/vapour samples collected from the inhalation chamber showed that the nominal concentration of 8.6 mg/l corresponded to a measured mean analytical dose of 6.6 mg/l.
- Duration of exposure:
- 4 h
- Concentrations:
- analytically detected: 6.6 mg/l; nominal dose: 8.6 mg/l
- No. of animals per sex per dose:
- 10
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weighing was done at the beginnibg of the study, on day 7 and 14. Observation was several times at the day of exposure and presumably daily exception of weekends and holidays afterwards.
- Necropsy of survivors performed: yes, at the end of the observation period, the animals were sacrificed and subjected to necropsy.
- Other examinations performed: clinical signs of toxicity - Statistics:
- No statistics were performed.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 6.6 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: No mortality
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 26.4 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 1 h
- Remarks on result:
- other: conversion according to Haber rule (C x t = k)
- Mortality:
- All rats survived.
- Clinical signs:
- other: Piloerection was reported for all rats.
- Body weight:
- No noticeable differences in body weight gain were seen between control and treated rats.
- Gross pathology:
- Necropsy revealed no abnormalities.
- Other findings:
- No other findings.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.