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EC number: 231-970-5 | CAS number: 7782-91-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-07-05 to 1990-08-15
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- guideline study - no microscopic examination were carry out even macroscopic abnormailties were found in animals. It was not clear if the abnormalities were found in animals surviving 24 or more hours in which case microscopic examination should have been carried out.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- adopted 24 February 1987
- Deviations:
- yes
- Remarks:
- No microscopic examination were carry out even macroscopic abnormailties were found in animals. It was not clear if the abnormalities were found in animals surviving 24 or more hours in which case microscopic examination should have been carried out.
- GLP compliance:
- yes
- Remarks:
- The study report states that the study was conducted in compliance with Good Laboratory Practice Standards, e.g. by the United Kingdom Compliance Programme, Department of Health & Social Security 1986 and subsequent revision, Department of Health, 1989.
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Molybdenum trioxide
- EC Number:
- 215-204-7
- EC Name:
- Molybdenum trioxide
- Cas Number:
- 1313-27-5
- Molecular formula:
- MoO3
- IUPAC Name:
- molybdenum trioxide
- Details on test material:
- - Name of test material (as cited in study report): Pure molybdic oxide
- Physical state: light grey powder
- Analytical purity: > 99.9 %, calcualted from reported Mo content (66.63%)
- Impurities (identity and concentrations): no relevant impurities > 1.0 %
- Purity test date: 1990-06-06
- Storage condition of test material: at room temperature
No further details on test material were stated.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CD
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: approximately four to six weeks of age in the main study
- Weight at study initiation: weight range of 110 to 131 g in the main study
- Fasting period before study: overnight prior to dosing
- Housing: housed in groups of up to five rats of the same sex in metal cages with wire mesh floors
- Diet (ad libitum): standard laboratory rodent diet (SDS LAD 1)
- Water (ad libitum): domestic quality potable water
- Acclimation period: minimum period of seven days prior to the start of the main study
ENVIRONMENTAL CONDITIONS
- Temperature (°C): mean daily minimum and maximum temperatures of the animal room were 22°C and 26°C respectively
- Humidity (%): mean daily relative humiditiy value was 59 % R.H.
- Air changes (per hr): approximately 15 air changes/hour
- Photoperiod (hrs dark / hrs light): 12 hour/12 hour
No further significant details on test animals were stated.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- Preliminary study:
A trial was carried out to establish a dosing regimen for the main study.
Pure molybdic oxide was prepared at various (w/v) concentrations in corn oil and administered at a volume of 10.0 ml/kg bodyweight. The test substance was prepared on the day of dosing.
Concentrations in vehicle: 20% w/v, 25 %w/v, 32 % w/v 40% w/v 50 % w/v (main study)
Main study:
The initial dose level was selected on the basis of the preliminary study. Further groups were dosed, after review of the results, to obtain a dose response curve and permit estimation of a median lethal dose.
Treatment procedure:
The appropriate dose volume of the test substance was administered to each rat using a syringe and plastic catheter (8 choke). The day of dosing was designated Day 1.
No further significant details on oral exposure were stated. - Doses:
- Preliminary study: 126, 500 and 2500 mg/kg mg/kg bodyweight
Main study: 2000, 2500, 3200, 4000 and 5000 mg/kg bodyweight - No. of animals per sex per dose:
- Preliminary study:2 males / 2 females
Main study: 5 males / 5 males (Exceptions: 2500 mg/kg bodyweigth only 5 males tested; 4000 mg/kg bodyweight only 5 females tested) - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: Preliminary study: 5 days; main study: 14 days; Test animals fasted approximately 4 hours after dosing.
- Frequency of observations and weighing: Preliminary study and main study: Animals were observed soon after dosing and at frequent intervals for the remainder of Day 1 (a period of six hours). On subsequent days the animals surviving treatment were observed once in the morning and again at the end of the experimental day. Clinical signs were recorded at each observation. Main study: The individual bodyweights of rats were recorded on Days 1 (day of dosing), 8 and 15 or at death.
- Necropsy of survivors performed: yes
- Other examinations performed: Main study: The nature, severity, approximate time of onset and duration of each toxic sign were recorded. Also the approximately time of death of individaul rats was recorded.
All surviving animals on the main study were killed on Day 15 by carbon dioxide asphyxiation. All animals that died during the study and those killed on Day 15 were subjected to a macroscopic post mortem examination which consisted of opening the cranial, abdominal anfd thoracic cavities. The macroscopic appearance of all examined tissues was recorded, and all livers and kidneys were preserved in buffered 10% formalin in order to satisfy any possible future requirement for further examination of these tissues.
No further information on study design were stated. - Statistics:
- The acute median lethal oral dose (LD50) to male and female rats was calculated using the method fo Finney (1971, Probit Analysis, §rd Edition, Cambridge University Press).
Separate LD50 values for males and females were estimated by undertaking probit analysis on the mortality data from the main study by fitting two parallel lines to the data (males only and females only) using the technique described by Finney (1978, Statistical Method in Biological Assay, 3rd Edition, Charles Griffin, London). A chi-square test was carried out to check that the data did not contain any evidence of non-parallelism.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 2 689 mg/kg bw
- 95% CL:
- >= 2 334 - <= 3 141
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 3 830 mg/kg bw
- 95% CL:
- >= 3 271 - <= 4 319
- Mortality:
- Deaths were seen in 1/5 male rats dosed at 2500 mg/kg bodyweight, in 5/5 males dosed at 3200 mg/kg, in 5/5 females dosed at 4000 mg/kg, and in 5/5 males and 4/5 females dosed at 5000 mg/kg. A majority of deaths occured from within 5 hours of dosing until Day 3. One female rat dosed at 4000 mg/kg was sacrificed in extremis on Day 4.
- Clinical signs:
- other: other: other: Pilo-erection was observed in all rats within five minutes of dosing and thoughout the remainder of Day 1. This sign was accompanied on Day 1 and/or at later intervals by: - Abnormal body carriage (hunched posture), abnormal gait (waddling)
- Gross pathology:
- Autopsy of rats that died during the study revealed dark kidneys in two males treated at 5000 mg/kg, and pale areas on the liver (in close proximity of the stomach) in one female rat dosed at 5000 mg/kg as the only macroscopic abnormalities.
Terminal autopsy revealed no macroscopic abnormalities.
Any other information on results incl. tables
Preliminary study:
The results of the preliminary study indicated that the acute median lethal oral dose to rats of Pure molybdic oxide was greater than 2500 mg/kg bodyweight.
Estimation of LD50 values
When probit analysis was carried out by fitting two parallel lines the values were:
Males 2689 (2334 to 3141) mg/kg bodyweight
Females: 3830 (3271 to 4319) mg/kg bodyweight
The slope of the parallel probit lines was 17.1 with a standard error of 4.8 using log. transformation of dose. The heterogeneity factor was not significant.
The mortality response curves: The difference between the lines for male and female rats was statistically significant (P< 0.005). Hence, there is a difference in the susceptibility of each sex to the test compound. A combined LD50 value was not therefore given since, as this is the mean value of the male and female LD50 estimations, 50% mortality would be expected, but would not actually occur, at this dose level.
The chi-square test for parallelism gave no evidence of non-parallelism.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
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