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Diss Factsheets
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EC number: 271-524-7 | CAS number: 68583-95-9 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 16035:1.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experiment test result performed using standard test guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- This study was designed to assess the toxic effects of the test compound on the ZEBRA FISH(Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- No data available
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in water. Therefore the stock solution prepared as 400mg/4L, with the concentration of 100mg/L, and was kept for 24 hr stirring. After this filter the stock, give it for analytical detection and then stock taken for experiment.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:ZEBRA FISH
- Source:UNIQUE AQUARIUM, NAGPUR
- Length at study initiation (length definition, mean, range and SD):average 2.09 cm
- Weight at study initiation (mean and range, SD):0.0688 g (average)
ACCLIMATION
- Acclimation period:7 days
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation: Daily once
- Health during acclimation (any mortality observed):During last 48 hours = no
FEEDING DURING TEST
- Food type: Standard brand feed
- Frequency: Withdrawal of food 1 day before and during exposure - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- 24, 48, 72 and 96 hours
- Hardness:
- 158.0
- Test temperature:
- 23.4 °C
- pH:
- 7.56
- Dissolved oxygen:
- 8.0 (mg/l)
- Nominal and measured concentrations:
- Nominal 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: plastic aquaria
- Type (delete if not applicable): open / closed: No data
- Material, size, headspace, fill volume: The test vessels used were all plastic aquaria having the water capacity of 5liters filled with 2 liter of water.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 16 hours light and 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Symptoms, pH, tempterature and Dissolved oxygen
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Nontoxic
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No other details known
- Details on results:
- No data available
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- No data available
- Sublethal observations / clinical signs:
Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
-
-
-
-
-
-
-
-
-
-
-
-
-
-
100
-
-
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
symptom (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on mortality of fish due to the exposure of test chemical at nominal concentration 100 mg/l, experimental median lethal Concentrations [LC-50 (96 h)] on Zebra fish(Danio rerio)was determine to be > 100 mg/l.
- Executive summary:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The stock solution was prepared as 400mg/4L, with the concentration of 100mg/L, and was kept for 24 hr stirring. After this filter the stock, give it for analytical detection and then stock taken for experiment.
The test was performed on the limit concentration i.e nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. Effect on the symptoms and the normal activity was checked in the interval of 24rs. pH, tempterature and dissolved oxygen was also checked.
The lethal concentrations LC50 was determine to be >100 mg/L
LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L
LC50 (96 hours) Experimental = >100 mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) =No mortality was observed
Thus based on the LC50 it can be concluded that the test chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.
Reference
Description of key information
Based on mortality of fish due to the exposure of test chemical at nominal concentration 100 mg/l, experimental median lethal Concentrations [LC-50 (96 h)] on Zebra fish(Danio rerio)was determine to be > 100 mg/l.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The stock solution was prepared as 400mg/4L, with the concentration of 100mg/L, and was kept for 24 hr stirring. After this filter the stock, give it for analytical detection and then stock taken for experiment.
The test was performed on the limit concentration i.e nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. Effect on the symptoms and the normal activity was checked in the interval of 24rs. pH, tempterature and dissolved oxygen was also checked.
The lethal concentrations LC50 was determine to be >100 mg/L
LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L
LC50 (96 hours) Experimental = >100 mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) =No mortality was observed
Thus based on the LC50 it can be concluded that the test chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.
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