2-methylpyridine

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP, non guideline study. Available as unpublished report. Restrictions in design and reporting but adequate for assessment.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Reference 2

Reference Type:

review article or handbook

Title:

SCREENING-LEVEL HAZARD CHARACTERIZATION Pyridine and Pyridine Derivatives Category

Author:

U.S. Environmental Protection Agency

Year:

2009

Bibliographic source:

http://www.epa.gov/hpvis/hazchar/Category%20Pyr%20and%20Pyr%20Derivs_Sept2009.pdf

Materials and methods

Principles of method if other than guideline:

0.5 mL of the test substance was applied to the skin of New Zealand White rabbits and covered with a patch for 4 hours. After exposure, the site was washed with water. Observations were made at removal of the test patch and at 24 and 48 hours.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-methylpyridine

EC Number:

203-643-7

EC Name:

2-methylpyridine

Cas Number:

109-06-8

Molecular formula:

C6H7N

IUPAC Name:

2-methylpyridine

Details on test material:

- Name of test material (as cited in study report): Alpha Picoline

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Housing: Stainless steel with elevated wire mesh flooring; 1 rabbit/cage
- Diet: Wayne 15% Rabbit Ration, ad libitum
- Water: Tap water, ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 15.6 - 23.9
- Humidity (%): 40-45
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:

occlusive

Preparation of test site:

clipped

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied: 0.5 mL

Duration of treatment / exposure:

4 hours

Observation period:

48 hours

Number of animals:

6

Details on study design:

TEST SITE
- Area of exposure: approximately one square inch.
- Type of wrap if used: Surgical gauze patches measuring not less than 1" x 1" and two single layers thick were placed over the treated area and impervious material was wrapped snugly around the trunk of the animal to hold the patch in place.

REMOVAL OF TEST SUBSTANCE
- Washing: yes, with water
- Time after start of exposure: 48 hours

SCORING SYSTEM:
Corrosion was considered to have resulted if the material in contact with the rabbit skin had caused destruction or irreversible alteration of the tissue. Tissue destruction was considered to have occurred if, at any of the readings, there was ulceration or necrosis. Tissue destruction was considered to have occurred if merely sloughing of the epidermis, erythema, edema or fissuring was evident. Observations of skin irritation, if any, encountered during testing of the material were noted.

Results and discussion

In vivo

Results

Remarks on result:

other: See "Irritant/corrosive response data"

Irritant / corrosive response data:

- 4 hours after exposure: Animal #1, 2, 3, 4 and 6 showed severe erythema, moderate edema and corrosion to the skin. Animal 5 showed slight erythema, moderate edema and no corrosion to the skin.
- 24 and 48 hours after exposure: Animal #1, 2 and 3 showed severe erythema, very slight edema and corrosion to the skin. Animal #4, 5 and 6 showed severe erythema, corrosion to the skin but no edema was observed.

Applicant's summary and conclusion

Interpretation of results:

corrosive

Remarks:

Migrated information