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EC number: 276-769-3 | CAS number: 72705-22-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study with GLP but only 4 strains were tested
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate
- EC Number:
- 276-769-3
- EC Name:
- Ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate
- Cas Number:
- 72705-22-7
- Molecular formula:
- C12H15NO6S.H3N
- IUPAC Name:
- ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate
- Details on test material:
- purity: 94.2 % (contains residual water)
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat S 9 (mix)
- Test concentrations with justification for top dose:
- with and without metabolic activation: 4, 20, 100, 500, 2500, 5000µg/plate
- Vehicle / solvent:
- double-distilled water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) and preincubation
DURATION
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: 3 - Evaluation criteria:
- A test compound is classified as mutagenic if it has either of the following effects:
a) it produces at least a 2-fold increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of revertants per plate of the appropriate vehicle control at complete bacterial background lawn.
b) it induces a dose-related increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of revertants per
plate of the appropriate vehicle control in at least two to three concentrations of the test compound at complete bacterial background lawn.
The assay is considered valid if the following criteria are met:
- the solvent control data are within the Iqboratory's normal control range for the spontaneous mutant frequency.
- the positive controls induced increases in the mutation frequency which were both statistically significant and within the laboratory's normal range. - Statistics:
- not required for an Ames-test
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The test compound did not precipitate on the plates up to the highest investigated dose of 5000 µg/plate.
The test compound proved to be not toxic to the bacterial strains.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and wihout metabolic activation
The results lead to the conclusion that the substance is not mutagenic in these bacterial test systems either in the absence or in the presence of
an exogenous metabolizing system. - Executive summary:
The substancewas tested for mutagenicity with the strains TA 100, TA 1535, TA 1537 and TA 98 of Salmonella typhimurium in an Ames-Test accordiung to OECD guideline 471.
Two independent mutagenicity studies were conducted, each in the absence and in the presence of an Aroclor-induced metabolizing system derived from a rat liver homogenate. For all studies, the compound was dissolved in double-distilled water, and each bacterial strain was exposed to 6 dose levels. Doses for all studies ranged from 4 to 5000 µg/plate.
Control plates without mutagen showed that the number of spontaneous revertant colonies was within the laboratory's historical control range. All the positive control compounds showed the expected increase in the number of revertant colonies.
Toxicity: In the mutagenicity experiments toxicity was not observed with and without metabolic activation up to the highest concentration.
Mutagenicity: In the absence and in the presence of the metabolic activation system the test item did not result in relevant increases in the number of revertants in any of the bacterial strains.
Summarizing, it can be stated that AE-Kresidinsulfosaures-NH4-Salz TTR was not mutagenic in this bacterial mutation test at any dose level in either the absence or in the presence of an exogenous metabolic activation.
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