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EC number: 443-090-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 13, 2001 to November 16, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: TOC determination at 0 and 72 h
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION OF STOCK SOLUTION
- Method: A stock solution was prepared by dissolving 125.4 mg of the test substance in 1 L of dilution water using a magnetic stirrer for 24 h. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Strain: Chodat
- Source (laboratory, culture collection): Non-axenic strain of the test species obtained from 'The Collection of Algal Cultures' of the Institute of Plant Physiology at the University of Göttingen (Germany)
- Age of inoculum (at test initiation): Pre-cultures were set up three days before the start of a test.
- Method of cultivation: The algal cultures for a test were taken from an exponentially-growing pre-culture and were mixed with the nutrient medium to make up to a final cell density of about 10E4 cells/mL in the test medium.
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21°C to 25°C
- pH:
- 7.7-10.4
- Nominal and measured concentrations:
- Nominal concentration: 3.1, 6.3, 13, 25, 50 and 100 mg/L
Measured concentration:
-Measured concentrations ranged from 100-130% of nominal values at 0 hours, and from 93-106% of nominal values at 72 hours, respectively.
-The test concentrations 3.1 mg/L and 6.3 mg/L were not analytically determined as the measured concentrations to be expected were below the quantization limit of the TOC determination method (2 mg/L).
-The measured concentration for the nominal concentrations of 13, 25, 50 and 100 mg/L were 16.9, 29.7, 59.3, 106 mg/L at 0 h; and 12.7, 25.4, 55.1 and 106 mg/L at 72 h. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 ml Erlenmeyer flasks with stoppers
- Culturing apparatus: Light chamber in which a temperature in the range 21 °C to 25°C can be maintained and continuous uniform illumination is provided in the spectral range 400 to 700 nm.
- Type (delete if not applicable): closed
- Initial cells density: 10E4 cells/mL
- Experimental design: 6 test concentrations with algae , 1 control (without test substance) with algae and 1 control (with highest test substance concentration) without algae.
- Test concentrations (nominal): 3.1, 6.3, 13, 25, 50 and 100 mg/L
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- Duration of exposure: 72 h
GROWTH MEDIUM
- Standard medium used: yes (OECD TG 201 medium)
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Criteria for effects: Adverse effects were monitored at 24, 48 and 72 h by determining the substance induced inhibition of growth [b] and growth rate [r] of the algal population. The 72 hour EC 50 (based on both, growth [b] and growth rate [r]), is calculated or read from the concentration/percentage response curve.
- Determination of cell density concentrations: microcell Microcell counter or microscopic counting chamber
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations (nominal): 3.1, 6.3, 13, 25, 50 and 100 mg/L - Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 27.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 57.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- other: EbC50
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 27.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 57.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Reported statistics and error estimates:
- Multisample comparison (according to DUNNETT 1955, 1964)
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the study, the 72 h EbC50 and ErC50 for Desmodesmus subspicatus were > 100 mg/L (equivalent to a measured concentration of > 106 mg/L). The NOECs for biomass and growth rate were determined to be 25 mg/L (equivalent to a measured concentration of 27.5 mg/L); the corresponding LOECs were 50 mg/L (equivalent to a measured concentration of 57.2 mg/L).
- Executive summary:
A study was conducted to determine the acute toxicity of the test substance to the algae Desmodesmus subspicatus according to EU Method C.3 (which is in most parts equivalent to OECD Guideline 201), in compliance with GLP.
Triplicate inocula of the algae were exposed to nominal concentrations of 0, 3.1, 6.3, 13, 25, 50 and 100 mg/L of the test substance for 72 h under static conditions. Quantification of the applied concentrations was carried out using Total Organic Carbon (TOC) determination. The organic components of the test substance corresponded to 66.1% of the molecular weight. No analytical determination was carried out at 3.1 and 6.3 mg/L as the measured concentrations to be expected were below the quantification limit of the TOC method (2 mg/L). Biomass and growth rate were determined after 0, 24, 48 and 72 h.
Under the conditions of the study, the 72 h EbC50 and ErC50 for Desmodesmus subspicatus were > 100 mg/L (equivalent to a measured concentration of > 106 mg/L). The NOECs for biomass and growth rate were determined to be 25 mg/L (equivalent to a measured concentration of 27.5 mg/L); the corresponding LOECs were 50 mg/L (equivalent to a measured concentration of 57.2 mg/L).
Reference
Percentage inhibition of cell growth (integral of biomass) and growth rate:
Nominal concentration of test substance (mg/L) |
Average of measured concentrations of test substance at 0 and 72 h (mg/L) |
Percentage inhibition of cell growth [b] |
Percentage inhibition of mean growth rate [r] |
Control |
- |
0.0 |
0.0 |
3.1 |
-* |
1.6 |
0.8 |
6.3 |
-* |
1.9 |
0.1 |
13 |
14.8 |
-0.9 |
1.1 |
25 |
27.5 |
2.1 |
0.4 |
50 |
57.2 |
11.1 |
2.8 |
100 |
106 |
14.7 |
3.3 |
* The test concentrations 3.1 mg/L and 6.3 mg/L were not analytically determined as the measured concentrations to be expected were below the quantization limit of the TOC determination method (2 mg/L).
Description of key information
Under the conditions of the study, the 72 h EbC50 and ErC50 for Desmodesmus subspicatus were > 100 mg/L (equivalent to a measured concentration of > 106 mg/L). The NOECs for biomass and growth rate were determined to be 25 mg/L (equivalent to a measured concentration of 27.5 mg/L); the corresponding LOECs were 50 mg/L (equivalent to a measured concentration of 57.2 mg/L).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 27.5 mg/L
Additional information
A study was conducted to determine the acute toxicity of the test substance to the algae Desmodesmus subspicatus according to EU Method C.3 (which is in most parts equivalent to OECD Guideline 201), in compliance with GLP.
Triplicate inocula of the algae were exposed to nominal concentrations of 0, 3.1, 6.3, 13, 25, 50 and 100 mg/L of the test substance for 72 h under static conditions. Quantification of the applied concentrations was carried out using Total Organic Carbon (TOC) determination. The organic components of the test substance corresponded to 66.1% of the molecular weight. No analytical determination was carried out at 3.1 and 6.3 mg/L as the measured concentrations to be expected were below the quantification limit of the TOC method (2 mg/L). Biomass and growth rate were determined after 0, 24, 48 and 72 h.
Under the conditions of the study, the 72 h EbC50 and ErC50 for Desmodesmus subspicatus were > 100 mg/L (equivalent to a measured concentration of > 106 mg/L). The NOECs for biomass and growth rate were determined to be 25 mg/L (equivalent to a measured concentration of 27.5 mg/L); the corresponding LOECs were 50 mg/L (equivalent to a measured concentration of 57.2 mg/L).
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