Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 264-129-6 | CAS number: 63405-85-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study initiation date - 20 August 1990; Experiment start date - 03 December 1990; Experiment end date - 11 January 1991; Study completion date - 25 February 1991.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Draft International Standard ISO/TC 147: "Water quality - Algal growth inhibition test, submitted June 25, 1987.
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Name: FAT 20004/H
Batch No: EN 317
Expiration Date: June 1995 - Analytical monitoring:
- yes
- Details on sampling:
- Analyses of the test substance were performed in duplicate in a sample of the stock solution and of test media of the nominal test concentrations 0.6, 2.5, and 10.0 mg/l mg test substance/l and the solvent control: a part of the freshly prepared stock solution and test media (without algae) were sampled and analysed.
For the determination of the stability of the test substance under test conditions, a part of the test media were incubated under the same conditions as in the test itself (but without algae) and analysed after the end of the test (96 hours). All samples will be kept stored at -20 °C until delivery of the final test report, pending on the decision of the sponsor for additional analyses. - Vehicle:
- not specified
- Details on test solutions:
- A stock solution was prepared by dissolving the test substance in Dimethylformamide p.A. (100 g/l). The stock solution was diluted in a series of sequential dilutions with the solvent to add the same volumes of solvent (0.1 ml/l) to each test solution. The final nominal concentrations of FAT 20004/H in the test media were: 0.6, 1.3, 2.5, 5.0 and 10.0 mg/l, a control without any additions and a solvent control (100 µl Dimethylformamide/l test medium).
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: Strain No. 8681 SAG
- Source (laboratory, culture collection): supplied by the Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen, D-3400 Göttingen, F.R.G. The algae were grown in the laboratories of RCC under standardized conditions according to the OECD Guideline No. 201 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Post exposure observation period:
- 72 hours
- Test temperature:
- 21.3 to 23.4 °C
- pH:
- 7.9 to 10.3
- Nominal and measured concentrations:
- The final nominal concentrations of FAT 20004/H in the test media were: 0.6, 1.3, 2.5, 5.0 and 10.0 mg/l, a control without any additions and a solvent control.
- Details on test conditions:
- Experimental Conditions
The algae were cultivated and tested in a test solution prepared according to the OECD Guideline No. 201.The experiment was started (0 hours) with a biomass of nominal 10,000 cells per ml test solution. These cells were taken from a sterile pre-culture, which was set up 72 hours prior to the test at the same conditions as in the test. Per test concentration, 150 ml of test solution (sterile nutrient solution, dissolved test substance and algae suspension) was incubated on a laboratory shaker in an air-conditioned room and by continuous illumination at 8000 Lux (±20 %) . This illumination was achieved by fluorescent tubes (universal white L 25, 36 W) , installed above the shaker in a distance of about 40 cm from the algae flasks. Incubation flasks used were 250 ml Erlenmeyer flasks stoppered with permeable plugs. The test design included three replicates of each test concentration and twice that number of controls. The test duration was 96 hours. As a means of detecting unsatisfactory test conditions the reference substance Potassium Dichromate p.A. has been periodically tested (twice a year). - Reference substance (positive control):
- not required
- Duration:
- 72 h
- Dose descriptor:
- EC0
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- In the control the cell density has increased from nominal N = 1 x 10E4 cells/ml at the start of the test (0 hours) to N = 304.4 x10E4 cells/ml after 96 hours by a factor of about 304, in the solvent control this factor amounted to about 27 8.8.
FAT 20004/H has obviously only a low inhibition effect on the growth of Scenedesmus subspicatus: first in the highest concentration tested of 10.0 mg test substance/l a marked reduction of cell densities and algae biomass (determined as the area under the growth curve) in relation to the solvent control was observed after 7 2 and 96 hours test duration, but for the growth rate u no clear concentration-effect-relation was observed up to this concentration. Therefore, the EC 0 (= NOEC: highest concentration tested without toxic effect) was determined to be 5.0 mg test substance/l, the LOEC (lowest concentration tested with toxic effect) amounted to 10.0 mg test substance/l. Due to the low inhibition rate in the concentration of 10 mg/l (as the highest concentration as possible to dissolve in Dimethyl formamid p.A at room temperature and an addition of 100 µl solvent stock solution to 1:1 test medium) no EC 50-values could be calculated. - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC0 was determined to be 5.0 mg test substance/L and the LOEC was determined to be 10.0 mg test substance/L.
- Executive summary:
The influence of FAT 20004/H on the growth of the green alga Scenedesmus subspicatus Chodat was investigated in a 96-hour static test according to the OECD Guideline No. 201, adopted June 7, 1984 and the Draft ISO/TC 147, submitted June 25, 1987. The nominal concentrations tested were 0.6, 1.3, 2.5, 5.0 and 10.0 mg test substance/l , a control without any additions and a solvent control group. FAT 20004/H had low inhibition effect on the growth of Scenedesmus subspicatus. In the highest concentration tested of 10.0 mg test substance/L a marked reduction of cell densities and algae biomass (determined as the area under the growth curve) in relation to the solvent control was observed after 72 and 96 hours test duration, but for the growth rate no clear concentration-effect-relation was observed up to this concentration. Therefore, the EC0 (= NOEC: highest concentration tested without toxic effect) was determined to be 5.0 mg test substance/l, the LOEC (lowest concentration tested with toxic effect) amounted to 10.0 mg test substance/l. Due to the low inhibition rate in the concentration of 10 mg/l no EC 50-values could be calculated. The test substance concentrations in the analysed test media amounted to 80 - 93.5 % of the nominal values. In the test media, incubated under test conditions (but without algae), the test substance was sufficiently stable. Therefore, the reported results are related to the nominal concentration of the test substance. The EC0 was determined to be 5.0 mg test substance/L and the LOEC amounted to 10.0 mg test substance/L.
Reference
Description of key information
The EC0 was determined to be 5.0 mg test substance/L and the LOEC amounted to 10.0 mg test substance/L. Hence, the LC50 is concluded to be greater than 100mg/L.
Key value for chemical safety assessment
Additional information
The influence of FAT 20004/H on the growth of the green alga Scenedesmus subspicatus Chodat was investigated in a 96-hour static test according to the OECD Guideline No. 201, adopted June 7, 1984 and the Draft ISO/TC 147, submitted June 25, 1987. The nominal concentrations tested were 0.6, 1.3, 2.5, 5.0 and 10.0 mg test substance/l, a control without any additions and a solvent control group. FAT 20004/H had low inhibition effect on the growth of Scenedesmus subspicatus. In the highest concentration tested of 10.0 mg test substance/L a marked reduction of cell densities and algae biomass (determined as the area under the growth curve) in relation to the solvent control was observed after 72 and 96 hours test duration, but for the growth rate no clear concentration-effect-relation was observed up to this concentration. Therefore, the EC0 (= NOEC: highest concentration tested without toxic effect) was determined to be 5.0 mg test substance/l, the LOEC (lowest concentration tested with toxic effect) amounted to 10.0 mg test substance/l. Due to the low inhibition rate in the concentration of 10 mg/l no EC 50-values could be calculated. The test substance concentrations in the analysed test media amounted to 80 - 93.5 % of the nominal values. In the test media, incubated under test conditions (but without algae), the test substance was sufficiently stable. Therefore, the reported results are related to the nominal concentration of the test substance. The EC0 was determined to be 5.0 mg test substance/L and the LOEC amounted to 10.0 mg test substance/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)