D-2-hydroxy-2-phenylacetic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

L-2-hydroxy-2-phenylacetic acid

EC Number:

210-276-6

EC Name:

L-2-hydroxy-2-phenylacetic acid

Cas Number:

611-71-2

Molecular formula:

C8H8O3

IUPAC Name:

hydroxy(phenyl)acetic acid

Details on test material:

TS-Freetext:
ChiPros (R)-Mandelsäure: (R)-mandelic acid (purity: 100 %)

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Test animals: Daphnia magna Straus
- Original source: IRCHA Institut National de Recherche Chimique Appliquée, France, 1978
- Age: 2-24 h (starting with the 3rd breed of parent animals)
- Feeding: no feeding during test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

- total hardness: 2.20-3.20 mmol/l

Test temperature:

19.1-19.8 °C

pH:

6.5 -8.2 (see table 2)

Dissolved oxygen:

8.4 -8.8 mg/L (see table 2)

Conductivity:

550-650 µS/cm

Nominal and measured concentrations:

test concentrations: 0 (control), 12.5, 25, 50, and 100 mg/l

Details on test conditions:

- test vessels: glass tubes with flat bottom (20 ml)
- test volume: 10 ml
- loading: 0.5 animals/ml
- 20 animals per concentration level

- illumination: artificial light, type warm white (OSRAM L58 W31)
- day/night rhythm: 16/8 h

- observation times: visually after 0, 24 and 48 h
- observation parameters: swimming ability

TEST MEDIUM
- type: M4 medium (ISO 10706)
- alkalinity to pH 4.3: 0.80-1.00 mmol/l
- molar ratio Ca:Mg: ca. 4:1
- aeration: for 24 h until saturation with oxygen

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Results with reference substance (positive control):

The EC50(24h) of the control substance potassium dichromate (non-GLP) was 1.08 mg/l (Date of the last control experiment: 08 July 2002 project number: 02/0106/50/6).

Any other information on results incl. tables

Table 1: Test concentration (retrieval, %):

Time (h)	minimum	maximum
0	106	114
48	106	106

 

Table 2: pH and dissolved oxygen of the test solutions after 0 and 48 h (measured in separate test vessel).

Test concentration (mg/L)	pH		dissolved oxygen (mg/L)
	0 h	48 h	0 h	48 h
control	8.2	8.1	8.8	8.7
12.5	8.1	8.1	8.7	8.6
25	8.1	8.1	8.7	8.5
50	7.2	8.0	8.7	8.5
100	6.5	7.9	8.6	8.4

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

In the control the immobilization was ≤ 10%. In the control no daphnids were captured in a surface film. The O2-concentration was > 3 mg/l in all tested replicates.

Conclusions:

No adverse effects of the test item to aquatic invertebrates were observed.

Executive summary:

An experimental study according to the OECD guideline 202 and GLP regulations was conducted to assess the toxicity of the test item to aquatic invertebrates. The test organism Daphnia magna was exposed the nominal test item concentrations of 0 (control), 12.5, 25, 50 and 100 mg/L. A separate non GLP study with the reference substance potassium dichromat was also conducted. 20 animals were exposed per concentration level for 48 h in a static test set-up. The mobility was observed visually after 0, 24 and 48 h. No effects on the mobility of the test organisms were observed during the test. All validity criteria were fulfilled.