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EC number: 271-678-5 | CAS number: 68603-87-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Bibliography: Ames et al., Mut.Research. 31: 347-364, 1975
Appropriate positive controls included - GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Carboxylic acids, di-, C4-6
- EC Number:
- 271-678-5
- EC Name:
- Carboxylic acids, di-, C4-6
- Cas Number:
- 68603-87-2
- Molecular formula:
- C5H8O4, C4H6O4, C6H10O4
- IUPAC Name:
- Carboxylic acids, C4-6 di-
- Details on test material:
- Sample idetification: AGS Mixture:
Adipic acid: 6-9%
Glutaic acid 30-35%
Succinic acid 8-11%
Nitric acid 0.5 - 3%
Lot: FIT-85-45,46,47; white solid powder
Constituent 1
- Specific details on test material used for the study:
- test item: AGS-Mixture (Adipic acid: 6-9%; Glutaric acid 30-35%; Succinic acid 8-11%; Nitric acid 0.5 - 3%) used as 50% aqueous solution
Method
- Target gene:
- Histidine auxotrothy
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 mix obtained from Aroclor 1254-treated Sprague Dawley rats
- Test concentrations with justification for top dose:
- 30, 100, 300, 1000, 3000 µg/plate
These dose levels were determined in a preliminary toxicity screen with 10, 167, 500, 1667 and 5000 µg/plate. - Vehicle / solvent:
- water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Water
- Positive controls:
- yes
- Positive control substance:
- other: Sodium acide, 9-aminoacridine, 2-nitrofluorene, 2-anthramine
- Details on test system and experimental conditions:
- plate incorporation assay
- Evaluation criteria:
- A positive result is defined as a reproducible, dose-related increase in the number of histidine-independent colonies with at least one dose point inducing a mutant frequency value that is two-fold the solvent control value.
- Statistics:
- Significance at the 95% conficence limits is determined by the program developed by Moore and Felton (1983).
Results and discussion
Test results
- Species / strain:
- other: all strains tested
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
Any other information on results incl. tables
Bacterial toxicity was investigated in a pilot experiment; no relevant colonies at 5000 µg/plate; high dose for main experiment: 3000µg/plate. All solvent and positive controls showed the expected results.
The results of the test article, AGS Mixture, were negative in strains TA 1535, 1537, 98 and 100 of S. typhimurium with and without metabolic activation at all doses tested (highest dose: 3000 µg/plate).
In the initial assay, strain 1538 exhibited an increase in mutant frequency at the hghest dose (2.5 fold above the concommitant control) without metaboilic activation. Two subsequent retests failed to confirm the initial observation (see Table 1)
Overall the authors concluded that the test compound is not mutagenic in the Ames test.
Table 1: summary of results
dose level (µg/plate) | S9 -mix ( + or -) | TA1535 | TA1537 | TA98 | TA100 | TA1538 (1.exp) | TA1538 (2. exp) | TA1538 (3.exp) |
water | - | 14 | 13 | 28 | 141 | 14 | 12 | 12 |
water | + | 9 | 11 | 44 | 159 | 20 | - | - |
sodium acide, 10 | - | 1107 | - | - | 1152 | - | - | - |
9 -Aminoacridine, 150 | - | - | 1496 | - | - | - | - | - |
2 -Nitrofluorene, 5 | - | - | - | 486 | - | 507 | 446 | 551 |
2 -Anthramine, 2.5 | + | 250 | 232 | 1544 | 1467 | 1436 | - | - |
AGS mix, 30 | - | 17 | 12 | 26 | 150 | 13 | 6 | 12 |
AGS mix, 100 | - | 13 | 10 | 24 | 133 | 15 | 8 | 12 |
AGS mix, 300 | - | 16 | 12 | 24 | 144 | 15 | 5 | 9 |
AGS mix, 1000 | - | 14 | 15 | 32 | 148 | 15 | 11 | 16 |
AGS mix, 3000 | - | 17 | 14 | 42 | 140 | 35 | 21 | 11 |
AGS mix, 30 | + | 10 | 10 | 38 | 119 | 18 | - | - |
AGS mix, 100 | + | 11 | 10 | 42 | 188 | 25 | - | - |
AGS mix, 300 | + | 8 | 13 | 34 | 190 | 13 | - | - |
AGS mix, 1000 | + | 8 | 18 | 43 | 181 | 31 | - | - |
AGS mix, 3000 | + | 11 | 14 | 42 | 197 | 30 | - | - |
AGS mixture was tested as 50% aqueous solution.
Applicant's summary and conclusion
- Executive summary:
Mutagenic activity of dicarboxylic acid mixture (AGS-Mixture, 50%) was investigated in a study similar to OECD TG 471 in bacteria (Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and TA1538) with or without metabolic activator S9. Cytotoxicity was observed at 5000 µg/plate. Negative and positive controls were functional in all experiments (Barfknecht 1986, BASF 1990).
In the initial assay, strain TA1538 exhibited a dose related increase in mutant frequency that reached 2.5-fold the solvent control background value without metabolic activation. However, two subsequent retests failed to confirm the initial observation. All other tests were negative. The authors concluded that dicarboxylic acid mixture is not mutagenic in the 5 strains tested with and without metabolic activation at dose levels of 30, 100, 300, 1000 and 3000 µg/plate.
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