Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acrylate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

Standard acute method. Study conducted pre-GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Test guideline

Guideline:

other: pre-guideline

GLP compliance:

no

Remarks:

Study conducted pre-GLP.

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acrylate

EC Number:

227-561-6

EC Name:

Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acrylate

Cas Number:

5888-33-5

Molecular formula:

C13H20O2

IUPAC Name:

(1S,2S,4S)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl prop-2-enoate

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Details on test animals or test system and environmental conditions:

Twelve male New Zealand albino rabbits, in a weight range of between 2.3 and 3.0 kilograms, were used in this experiment. The animals were fed, housed, and maintained in accordance with standard laboratory procedures.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The animals were immobilized in an animal holder and the trunks clipped free of hair with an Oster animal clipper. One-half of the animals were further prepared by making epidermal abrasions every two or three centimetres longitudinally over the area of exposure. The abrasions were made sufficiently deep to penetrate the stratum corneum but not deep enough to disturb the derma. The test material was introduced over approximately 10.7 % of the animal body surface and held in contact with skin by means of a sleeve for a period of twenty-four hours at which time the sleeve was removed and the treated areas wiped clean of the remaining excess of the material.

Duration of exposure:

24 hrs

Doses:

3000 mg/kg

No. of animals per sex per dose:

12

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

> 3 000 mg/kg bw

Based on:

test mat.

Mortality:

no

Clinical signs:

other: not reported

Gross pathology:

not reported

Other findings:

not reported

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Remarks:

1272/2008/EU Criteria used for interpretation of results: EU

Conclusions:

According to the test result: LD50(14days): > 3000 mg/kg bw (male rats) the test substance Isobornyl acrylate has not to be classified with respect to acute dermal toxicity (EU GHS criteria; 1272/2008/EU).

Executive summary:

In an acute dermal toxicity study according to standard method conducted pre-GLP, a group of fasted male New Zealand rabbits was administered dermally a single oral dose of 3000 mg/kg body weight Isobornyl acrylate. The observation period was 14 days.

 Result:

Dermal LD₅₀ >3000 mg/kg bw   

Isobornyl acrylate has a low acute toxic potential in rabbits (EU GHS: no category) based on this test.