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EC number: 416-600-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-10-11 to 2001-01-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Analytical purity: 97.4 %
Lot/batch No.: July 30, 1999 - Analytical monitoring:
- not specified
- Details on sampling:
- Preparation of test solutions:
In order to improve the substance dispersing in water, the test substance was ground down to very fine particles using a mortar and pestle before weighing. As the solubility of the test substance in Water is very low, test solutions at 31.6 and 100 mg/L were prepared by direct addition of the test substance in the test vessels. Test solutions at 1, 3.13 and 10 mg/L were prepared by means of a suspension at 20 mg/L in dechlorinated water because test substance quantities necessary for direct addition to the test flasks were too low to be accurately weighed (< 10 mg). The suspension was agitated during 8 minutes. Test solutions (1, 3.16 and 10 mg/L) were prepared immediately by further dilution of the suspension, maintained under agitation, with dechlorinated water. - Vehicle:
- no
- Details on test solutions:
- The test solutions were prepared as follows:
- two control solutions: activated sludge in dechlorinated water to obtain a final sludge concentration of 1.6 g/L,
- five test substance solutions: activated sludge in dechlorinated water (at 1.6 g/L) and test substance (to obtain final concentrations of 1, 3.16, 10, 31.6 and 100 mg/L),
- three reference substance (3,5-dichlorophenol) solutions: activated sludge in dechlorinated water (at 1.6 g/L) and reference substance (to obtain final concentrations of 4, 12 and 36 mg/L). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type: Activated sludge collected from a water treatment works containing effluent from a predominantly domestic origin;
Origin: The water treatment plant Emereaude (SIARR) (76141 Petit-Quevilly, France); - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 280 ± 20 mg/L as CaCO3
- Test temperature:
- 20 ± 2 °C
- pH:
- 6.0 - 8.0
- Dissolved oxygen:
- Not indicated
- Salinity:
- Not indicated
- Nominal and measured concentrations:
- Nominal concentrations: 1; 3.16; 10, 31.6; 100 mg/L of test substance; inoculum: 1.6 g/L;
Measured concentrations: Analytical measurement was not performed. - Details on test conditions:
- Environmental conditions during the test:
Temperature: between 20 and 22 °C;
Illumination: no special precautions were taken;
Dilution water: dechlorinated tap water was used, with a hardness of 280 ± 20 mg/L as CaCO3;
Duration of test: 3 hours;
Aeration: air was bubbled through each solution at the rate of 0.5 to 1 L/min until the start of oxygen concentration measurement;
Test item concentrations: 1; 3.16; 10, 31.6; 100 mg/L of test substance; inoculum: 1.6 g/L; - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Validity criteria:
As the difference between the two controls was below 15 % and the EC50 of 3,5-dichlorophenol was between 35 and 30 mg/L, the test was therefore considered valid.
Determination of the EC50:
Respiration rates of the test and reference solutions are presented in the table (See Sect. " Remarks on results incl. tables and figures")
The respiration rate of the test solution of highest concentration (100 mg/L) was equivalent to the respiration of the first control (i.e. these rates were within 15 % of each other). Therefore, the oxygen consumption rate of the for other test solutions (1 to 31.6 mg/L) was not determined.
The EC50, based on nominal concentrations, were as follows:
- Test substance: 3-hour EC50 (mg/L): > 100;
- Reference substance: 3-hour EC50 (mg/L): 10.8 (95 % confidence limits (mg/L): 8.87 - 13.1; - Results with reference substance (positive control):
- Reference substance: 3-hour EC50 (mg/L): 10.8
- Reported statistics and error estimates:
- Reference substance: 3-hour EC50 95 % confidence limits (mg/L): 8.87 - 13.1;
- Validity criteria fulfilled:
- yes
- Conclusions:
- HAT ISO was tested for toxicity to bacteria in a 3-hour activated sludge respiration inhibition test. The EC50 of the test item HAT ISO was found to be > 100 mg/L. HAT ISO is considered as non-toxic for aquatic microorganisms. No classification and labelling for aquatic toxicity is required according to Regulation 1272/2008/EEC (CLP).
- Executive summary:
HAT ISO was tested for toxicity to bacteria in a 3-hour activated sludge respiration inhibition test.
The EC50, based on nominal concentrations, were as follows:
- Test substance: 3-hour EC50 (mg/L): > 100;
- Reference substance: 3-hour EC50 (mg/L): 10.8
(95 % confidence limits (mg/L): 8.87 - 13.1);
HAT ISO is considered as non-toxic for aquatic microorganisms.
Reference
Respiration rates of the test and reference solutions are presented in the table.
Table: Respiration rate in mg O2-L-1.h-1.
|
Concentration (mg/L) |
Respiration rate in mg O2-L-1.h-1. |
Percent inhibition (%) |
Control 1 |
0 |
42.0 |
|
Control 2 |
0 |
45.0 |
|
|
|
|
|
Reference substance |
4 |
34.8 |
20 |
|
12 |
19.5 |
55 |
|
36 |
7.5 |
83 |
|
|
|
|
PATE HAT SECHEE |
1 |
ND |
|
|
3.16 |
ND |
|
|
10 |
ND |
|
|
31.6 |
ND |
|
|
100 |
46.5 |
0 |
|
|
|
|
ND: not determined
Description of key information
HAT ISO was tested for toxicity to bacteria in a 3-hour activated sludge respiration inhibition test.
The EC50, based on nominal concentrations, were as follows:
- Test substance: 3-hour EC50 (mg/L): > 100;
- Reference substance: 3-hour EC50 (mg/L): 10.8
(95 % confidence limits (mg/L): 8.87 - 13.1);
HAT ISO is considered as non-toxic for aquatic microorganisms.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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