Registration Dossier
Registration Dossier
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EC number: 212-110-8 | CAS number: 763-32-6
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study. Comparable to OECD 202.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EG guideline 79/831/EWG, Annex V, part C
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-methylbut-3-en-1-ol
- EC Number:
- 212-110-8
- EC Name:
- 3-methylbut-3-en-1-ol
- Cas Number:
- 763-32-6
- Molecular formula:
- C5H10O
- IUPAC Name:
- 3-methylbut-3-en-1-ol
- Details on test material:
- - Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
- Lot/batch No.: 31-0342
- Analytical purity: 99%;
water solubility: > 500 mg/l at 20 °C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (nominal 500 mg/l) in culture medium was prepared. Then, this test concentration was serially diluted with culture medium to obtain the desired test concentrations.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.70 +/- 0.5 mmol/l
- Test temperature:
- 19 - 21 °C
- pH:
- 8.0 - 8.2
- Dissolved oxygen:
- 8.3 - 9.1 mg/l
- Nominal and measured concentrations:
- Nominal test concentrations (mg/l):
31.25,
62.5,
125,
250,
500,
control - Details on test conditions:
- TEST SYSTEM
- Test vessel: flat bottom glass tubes (nominal 20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Mixture of dechlorinated tap water and distilled water, acidified with H2SO4 (p.a.)
- Molar ratio Na:K: about 10 : 1
- Alkalinity up to pH 4.3: 0.8 +/- 1.0 mmol/l
- Ca/mg ratio: 4 : 1
- Conductivity: 550 - 650 microSiemens/cm
OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours
- Light intensity: diffuse light (10 microeinstein/m*m*s) at 400 - 750 nm wave length
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Inhibition of swimming ability of daphnids after 3, 6, 24 and 48 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- Probit analysis
Any other information on results incl. tables
No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) 0 / 3 / 6 / 24 / 48
Control / 0 / 0 / 0 / 0 / 0
31.25 / 0 / 0 / 0 / 0 / 0
62.5 / 0 / 0 / 0 / 0 / 0
125 / 0 / 0 / 0 / 0 / 0
250 / 0 / 0 / 0 / 0 / 0
500 / 0 / 0 / 0 / 0 / 10
24 hour ECx values:
EC0: >= 500 mg/l
EC50: > 500 mg/l
EC100: > 500 mg/l
All biological results refer to nominal test concentrations.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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