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EC number: 247-045-4 | CAS number: 25498-49-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-study according to OECD guideline 401.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- [2-(2-methoxymethylethoxy)methylethoxy]propanol
- EC Number:
- 247-045-4
- EC Name:
- [2-(2-methoxymethylethoxy)methylethoxy]propanol
- Cas Number:
- 25498-49-1
- Molecular formula:
- C10H22O4
- IUPAC Name:
- [2-(2-Methoxymethylethoxy)methylethoxy]propanol
- Details on test material:
- "Dowfroth 250E" (polypropylene glycol methyl ether)
Batch No.: 120286 21.3.86
Purity: 90% polypropylene glycol methyl ether (tri- and higher).
Supplied as: Metal screw-top container.
Appearance : Clear brown liquid.
Administered as : Pure liquid.
Specific Gravity : 0.973.
Solubility: Not specified.
Stability : Not specified.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Sprague-Dawley CFY
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Interfauna (UK) limited, Wyton, Huntingdon
- Age at study initiation: Males: 121-151 g and females: 101-136 g
- Weight at study initiation: Approximately five to eight weeks old
- Fasting period before study: overnight fasting immediately before dosing
- Housing: The animals were housed in groups of up to five by sex in polypropylene cages with sawdust bedding
- Diet (e.g. ad libitum): Rat and Mouse Expanded Diet No. 1, Special Diet Services Limited, Witham, Essex, U. K.
- Water (e.g. ad libitum): Drinking water ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-22º C
- Humidity (%): 45-65 %
- Air changes (per hr): 15 changes per hour
- Photoperiod (hrs dark / hrs light): 12 hours light and 12 hours darkness
IN-LIFE DATES: not specified
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: none
- Details on oral exposure:
- Four groups of Sprague-Dawley rats (5/sex/dose level) received single oral doses of 2300, 3000, 3900, or 5000 mg/kg polypropylene glycol methyl ether (PPM), administered undiluted using a stainless steel stomach cannula attached to a syringe. Animals were fasted overnight prior to dosing. The specific gravity was determined and used to calculate the appropriated dose volumes for the required dose levels.
- Doses:
- 2300, 3000, 3900 and 5000 mg/kg
- No. of animals per sex per dose:
- 5 rats/sex/dose
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were observed 1 and 4 hours after dosing and subsequently once daily for 14 days. Individual body weights were recorded on the day of treatment (day 0), days 7 and 14 and at death.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathological examinations - Statistics:
- LD50's and 95% confidence limits were calculated for both sexes individually and combined using the method of Weil (Biometrics 8:249, 1952).
Results and discussion
- Preliminary study:
- A preliminary study was performed using groups of two rats (one male and one female) to determine suitable dose levels for the main study. The doses were 500, 1000, 2000, 4000 and 8000 mg/kgbw. Animals were observed 1 and 4 hours after dosing and then daily for five days, or until all evidence had subsided, whichever was longer. Bodyweights were recorded on the day of dosing. No necropsies were performed. Mortalities occured at the dose level of 8000 mg/kgbw (2/2). The mortalities indicated an oral LD50 of between 4000 and 8000 mg/kg.
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 500 mg/kg bw
- 95% CL:
- >= 3 100 - <= 3 900
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 3 600 mg/kg bw
- 95% CL:
- >= 3 100 - <= 4 200
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 3 400 mg/kg bw
- 95% CL:
- >= 2 900 - <= 4 100
- Mortality:
- Males:-
2300 mg/kg: 0/5
3000 mg/kg: 1/5
3900 mg/kg: 3/5
5000 mg/kg: 5/5
Females:-
2300 mg/kg: 0/5
3000 mg/kg: 2/5
3900 mg/kg: 3/5
5000 mg/kg: 5/5 - Clinical signs:
- other: Symptoms in survivors exhibited a dose-response relationship and included hunched posture, lethargy, piloerection, decreased respiratory rate, ptosis, body tremors, and red/brown staining around the eyes or snout at all dose levels. Other more severe
- Gross pathology:
- Necropsy of survivors revealed congested lungs in 4 males and 4 females from the 2300 mg/kg group but not in the 3000 or 3900 mg/kg groups.
- Other findings:
- None
Any other information on results incl. tables
Mortality is shown in the table below:
Dose Level(mg/kg) | Mortalities |
|||
Male | Female | Both Sexes | Percentage | |
2300 | 0/5 | 0/5 | 0/10 | 0 |
3000 | 1/5 | 2/5 | 3/10 | 30 |
3900 | 3/5 | 3/5 | 6/10 | 60 |
5000 | 5/5 | 5/5 | 10/10 | 100 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- With an oral LD50 of 3500 mg/kg, polypropylene glycol methyl ether shows a low degree of acute toxicity. This test material is appears to be comprised largely of tripropylene glycol methyl ether.
- Executive summary:
Twenty five male and twenty five female Sprague Dawley CFY strain rats were used. Animals were acclimatized for a minimum period of five days. At the start of the main study the males weighed 121-151 g, and the females 101-136 g, and were approximately five to eight weeks old. The animals were housed in groups of up to five by sex in polypropylene cages with sawdust bedding. With the exception of an overnight fast immediately before dosing and for approximately two hours after dosing, free access to mains drinking water and food was allowed throughout the study.
The animal room was maintained at a temperature of 19-22º C and relative humidity of 45-65%. The rate of air exchange was approximately 15 changes per hour.
Following an initial range-finding study, four groups, each of ten rats (five males and five females) were given a single oral dose of test material at dose levels of 2300, 3000, 3900 and 5000 mg/kg bodyweight.
Animals were observed 1 and 4 hours after dosing and subsequently once daily for 14 days. Mortalities and evidence of overt toxicity were recorded at each observation. Individual bodyweights were recorded on the day of treatment (day 0), days 7 and 14 and at death. All animals were subjected to gross necropsy examination for any microscopic abnormalities. No tissues were retained.
Symptoms in survivors exhibited a dose- response relationship and included hunched posture, lethargy, piloerection, decreased respiratory rate, ptosis, body tremors and red/ brown staining around the eyes or snout at all dose levels. Other more severe symptoms occurring more often at higher dose levels included coma and gasping respiration. By day 2, symptoms had disappeared in the two lower dose levels and by day 3 in survivors from the 3900 mg/kg group.
Deaths occurred in a dose related manner (3/10 at 3000 mg/kg, 6/10 at 3900 mg/kg and 10/10 at 5000 mg/kg). All rats which died did so within 24 hours of dosing and survivors recovered from the non-specific signs of toxicity within 3 days of dosing. Some survivors were reported to have congested lungs at necropsy after 14 days observation but most were unremarkable.
The LD50 in rats for Dowfroth 250E was 3600 mg/kg for males, 3400 mg/kg for females and 3500 mg/kg for the sexes combined (3100-3900 mg/kg 95% confidence limits). As the LD50 of the test material was greater than 2000 mg/kg, Dowfroth 250E was not classified as toxic as per EU classification.
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