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EC number: 221-374-3 | CAS number: 3081-01-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study meets generally accepted scientific principles Lack of analytical monitoring data. Effect values should not be considered for risk evaluation.
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-660/3-75-009
- Deviations:
- no
- Principles of method if other than guideline:
- EPA Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. Environmental Protection Agency, Ecological Research Series
EPA-660/3-75-009, April, 1975. 61 p.
American Public Health Association. 1975. Standard Methods for the Examination of Water and Wastewater. 14th ed. Washington, DC. 1193 p. - GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- Test concentrations were prepared based on total compound. Nanograde acetone was used in the preparation of all working stock solutions. The solvent control received an aliquot 0.01 mL. of acetone equivalent to that of the highest test concentration.
- Vehicle:
- yes
- Details on test solutions:
- Aceton
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna cultured at the ABC facilities.
Feeding with algae (Selenastrum capricornutum)
The daphnids were identified to species using the taxonomic key presented by Pennak. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Hardness:
- 255 mg CaCO3
- Test temperature:
- 21 °C
- pH:
- 6.9 - 7.8
- Dissolved oxygen:
- 6.6 - 7.4
- Nominal and measured concentrations:
- nominal conc.: 0.1, 0.18, 0.32, 0.56 and 1.0 mg/L
- Details on test conditions:
- An initial range finding experiment was conducted using 5 Daphnia each in exposure concentrations of .01, 0.1, 1.0 mg/L. From this infor mation, five concentrations in duplicate of the test compound with ten Daphnia (first instar less than 24 hours old) per beaker were selected for their respective bioassay. These concentrations were a logarithmic series ranging from 0.10 to 1.0 mg/L and included a control and solvent control. All concentrations were observed once every 24 hours for mortality and abnormal effects such as surfacing, clumping of the daphnia together and daphnids lying on the bottom of test chambers.
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 0.10 -0.32 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Reference
Description of key information
The acute toxicity of 7PPD to Daphnia magna was investigated in a static test according to EPA-660/3-75-009. After 48 hours of exposure an EC50 of 0.2 mg/L (nominal) was determined.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.2 mg/L
Additional information
Short-term tests have been performed with 7PPD as well as with other similar PPDs yielding EC50-values in the range of 0.2 to 1.7 mg/L. The result of the study performed with 4-HDPA (0.69 mg/L) is within this range. N-(1,4 -dimethylpentyl)-N'-phenylbenzene-1,4 -diamine (7PPD) is hydrolytically unstable at pH 7 and p-Hydroquinone CAS 123 -31 -9 was identified as the most sensitive (secondary) hydrolysis product. The lowest effect value in acute tests was found for p-Hydroquinone (0.134 mg/L) which is close to the values for the PPDs (0.2 to 1.9 mg/L) and 4-HDPA (0.69 mg/L). p-Hydroquinone is a secondary hydrolysis product of 7PPD, athough having the lowest effect concentration this value was not chosen as key value as the hydrolysis from 7PPD to p-Hydroquinone is rather slow.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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