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EC number: 203-685-6 | CAS number: 109-59-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1970
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Non-guideline non-GLP study, with adequate and well described methods and results.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 970
- Reference Type:
- publication
- Title:
- The metabolism of isopropyl oxitol in rat and dog
- Author:
- Hutson, D.H. and Pickering, B.A.
- Year:
- 1 971
- Bibliographic source:
- Xenobiotica, 1, 105-119.
Materials and methods
- Objective of study:
- excretion
- metabolism
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- no
Test material
- Reference substance name:
- 2-isopropoxyethanol
- EC Number:
- 203-685-6
- EC Name:
- 2-isopropoxyethanol
- Cas Number:
- 109-59-1
- Molecular formula:
- C5H12O2
- IUPAC Name:
- Propan-2-ol
- Details on test material:
- - Name of test material (as cited in study report): O-Isopropyl [1,2-14C] ethanediol/ [1,2-14C]isopropyl oxitol.
- Specific activity (if radiolabelling): 9.6 uCi/mg
Constituent 1
- Radiolabelling:
- yes
Test animals
- Species:
- rat
- Strain:
- other: Carworth Farm E strain
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Carworth Farm E strain
- Weight at study initiation: 200-250g body wt.
- Housing:The rat were housed for four days in Jencons all-glass metabolism cages as described by Wright, et al.(1965) and had free access to food and water
- Individual metabolism cages: yes
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- physiological saline
- Duration and frequency of treatment / exposure:
- Single exposure
Doses / concentrations
- Dose / conc.:
- 0.933 other: mg
- Remarks:
- 9.55uCi in 0.5 ml of 0.9% sterile saline.
- No. of animals per sex per dose / concentration:
- 6
- Control animals:
- not specified
- Details on study design:
- Respired air from each animal was drawn (400ml/min) through a trap containing 500ml 5M NaOH. These traps were changed every 24 hrs for 4 days. After 4 days the animals were killed by ether anaesthesia and were skinned (the feet, muzzle and tail remaining with the skin). The alimentary tracts, from anus to oesophagus, were then removed for separate analysis.
- Details on dosing and sampling:
- METABOLITE CHARACTERISATION STUDIES
- Tissues and body fluids sampled (delete / add / specify): Urine and faeces were collected daily. All samples were stored at - 20 degrees C until required for assay. Daily urine samples were directly assayed for radioactivity by scintillation counting. Faeces and tissues were homogenized with water and duplicate samples combusted in oxygen, the resulting CO2 being absorbed directly into scintillator solution. The CO2 content of the NaOH traps was determined by releasing CO2 from 1 mL duplicate samples of soln. by the addition of 5M-H2SO4, and trapping the released gas in 5 mL of phenylethylamine. The other components of the scintillator were then added to the phyeylethylamine and the sample assayed for radioactivity.
- Measurement of radioactivity: Aliquots of solution to be assayed for radioactivity were blended with 15ml of phenyl ethylamine scintillator solution based on that devised by Dobbs (1963) and the radioactivity determined using a Packard Tricarb Liquid Scintillation Scpectrometer (Model 3003).
- Method type(s) for identification: The 0-24h urine from the rats was pooled and analysed directly by t.l.c. in comparison with 2-isopropoxyethanol and isopropoxyacetic acid.
Results and discussion
- Preliminary studies:
- -In a preliminary experiment with one animal, the respired air was drawn through acetone at -20 degrees C in order to trap respired radioactive metabolites, if any. After 12h, the acetone was radioassayed. An amount of radioactivity corresponding to less than 0.2% of the dose was recovered in the trap.
Toxicokinetic / pharmacokinetic studies
- Details on absorption:
- No data
- Details on distribution in tissues:
- Skin: 3.4% of administered radioactivity (4.4% males, 2.4% females)
Gut: 0.7% of administered radioactivity (0.8% males, 0.6% females)
Carcass: 6.1% of administered radioactivity (7.2% males, 5.0% females)
- Details on excretion:
- The metabolism of 2-isopropoxyethanol was similar in the dog and the rat. In the rat, this material was rapidly metabolized and 88% was excreted from the body in 24h (average for male and female), 73% via the urine, and 14% via the lungs as carbon dioxide. See Table 1 in Remarks.
Metabolite characterisation studies
- Metabolites identified:
- yes
- Details on metabolites:
- The major urinary metabolites were isopropoxyacetic acid (30% of urinary radioactivity), N-isopropoxyacetylglycine(46% of urinary radioactivity), and ethylene glycol (13% of urinary radioactivity). Only the first two metabolites were identified in the urine from beagle hound treated with this glycol ether. The occurrence of the glycine derivative of isopropoxyacetic acid was unexpected and suggests conjugation of the acid within the mitochondrion by glycine N-acylase. Based on these results, the authors suggest the metabolic scheme shown in Figure 1 for the rat.
Any other information on results incl. tables
There was no difference between results calculated by gaseous transfer of the carbon dioxide traps and results calculated by direct sampling of the traps,thus all radioactivity exctreted via the lungs was [14C]carbon dioxide, and no other volatile metabolite was excreted.
Table 1. Daily levels of radioactivity in urine, faeces, and respired air after the administration of [14C]isopropyl oxitol to rats
Period |
Males |
Females |
||||
Urine |
Faeces |
CO2 |
Urine |
Faeces |
CO2 |
|
0-24 h |
65.5 (3.28) |
4.3 (1.64) |
10.1 (0.44) |
76.1 (4.87) |
4.6 (2.59) |
7.9 (0.47) |
24-48 h |
1.2 (0.23) |
0.1 (0.07) |
4.0 (0.30) |
2.1 (0.41) |
0.1 (0.06) |
2.9 (0.22) |
48-72 h |
0.4 (0.04) |
0.1 (0.08) |
1.4 (0.04) |
0.6 (0.08) |
0.1 (0.03) |
1.0 (0.07) |
72-96 h |
0.3 (0.03) |
0.3 (0.06) |
0.8 (0.06) |
0.4 (0.08) |
0.2 (0.06) |
0.4 (0.04) |
Totals |
67.4 (3.1) |
4.8 (1.7) |
16.3 (0.4) |
79.2 (4.4) |
5.0 (2.6) |
12.2 (0.6) |
Results expressed as % of the administered radioactivity and (S.E.M. values are shown in parenthesis).
Applicant's summary and conclusion
- Conclusions:
- low bioaccumulation potential based on study results. The test substance is rapidly metabolised and 88% excreted within 24 hrs
- Executive summary:
In this study a single intraperitoneal injection of [1,2-14C]isopropyl oxitol was administered to rats and its metabolism and excretion determined. The material was rapidly metabolised and 88% was excreted from the body within 24hrs in the rat, 71% through kidneys, and 12% via the lungs as carbon dioxide. Radioactivity found in the animal tissues at the end of the experiment accounted for 10% of the adminstered dose. The major urinary metabolites were isopropoxyacetic acid (30% of urinary radioactivity), N-isopropoxyacetylglycine(46% of urinary radioactivity), and ethylene glycol (13% of urinary radioactivity).
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