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EC number: 228-846-8 | CAS number: 6362-80-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to GLP and guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes
- Test type:
- acute toxic class method
Test material
- Reference substance name:
- 1,1'-(1,1-dimethyl-3-methylene-1,3-propanediyl)bisbenzene
- EC Number:
- 228-846-8
- EC Name:
- 1,1'-(1,1-dimethyl-3-methylene-1,3-propanediyl)bisbenzene
- Cas Number:
- 6362-80-7
- Molecular formula:
- C18H20
- IUPAC Name:
- (4-methyl-4-phenylpent-1-en-2-yl)benzene
- Details on test material:
- - Name of test material (as cited in study report): 2,4-diphenyl-4-methyl-1-pentene
- Physical state: Liquid
- Analytical purity: 96.97%
- Lot/batch No.: 412220
- Impurities (identity and concentrations): 2,4-Diphenyl-4-methyl-2-pentene 2.54%
- Storage condition of test material: Room temperature protected from light
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories Japan (Hino Breeding Center)
- Age at study initiation: 8 weeks of age
- Weight at study initiation: 175-187 g.
- Fasting period before study: Animals were fasted from the evening of the day before administration (from 4:00-4:10 pm) for about 17.5-18.5 hours until administration, and then for about 6 hours after administration, after which feed was provided again
- Housing: The animals were housed individually in racks of 5 stainless steel cages (W: 755 × D: 210 × H: 170 mm) during both the quarantine/acclimation period and after grouping
- Diet: The animals had free access to pellet diet (CRF-1, Oriental Yeast Co., Ltd.)
- Water: The animals were provided free access to tap water in water bottles. However, water was removed after grouping for about 6 hours until administration, after which it was provided again.
- Acclimation period: A five day quarantine period followed by a 2 day acclimation period was set for the animals before the first, second, third and fourth tests
ENVIRONMENTAL CONDITIONS
- Temperature: 23°C (measured values: 21-23°C),
- Humidity: nominal humidity of 55% (measured values: 40-61%)
- Air changes (per hr): 12 times/hour (filter-sterilized fresh air).
- Photoperiod: 12-hour light/dark cycle (illumination: 6:00 am to 6:00 pm)
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED:
The dose volume was 10 mL/kg, calculated based on the body weight measured immediately before administration.
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: The LD50 value for oral administration of 2,4-diphenyl-4-methyl-1-pentene in rats is reported to be 3.8 mL/kg. 1) Therefore, the maximum dose of 2000 mg/kg according to the OECD Guideline for Testing of Chemical for Acute Oral Toxicity-Acute Toxic Class method (Revised Guideline 423) was selected for the first test. - Doses:
- 2000 and 300 mg/kg
- No. of animals per sex per dose:
- Three females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The animals were observed for clinical signs and mortality for 6 hours after administration (immediately after to 30 minutes after administration and 2, 4 and 6 hours after administration) and once a day during the observation period from the day after administration. Body weight was measured on the day of administration (immediately before administration) and 1, 3, 7, 10 and 14 days after administration
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 300 - 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- One animal died on day 1 after administration in the first test at 2000 mg/kg, and 2 animals died on day 1 after administration in the second test at 2000 mg/kg. There were no deaths in the third and fourth tests at 300 mg/kg. From these results, the LD50 value of 2,4-diphenyl-4-methyl-1-pentene was estimated to be between 300 mg/kg and 2000 mg/kg.
- Clinical signs:
- other: Clinical signs observed in the first test at 2000 mg/kg were tremor in 1 animal at 4 hours after administration, tremor and clonic convulsions in 1 animal at 6 hours after administration, and soiled perineal region in 2 animals and clonic convulsions in 1
- Gross pathology:
- No abnormal necropsy findings were observed either in the animals that died in the first and second tests at 2000 mg/kg.
No abnormal necropsy findings were observed in the surviving animals in the first and second tests at 2000 mg/kg or in the surviving animals in the third and fourth tests at 300 mg/kg.
Any other information on results incl. tables
Results: (Test group 1: 1,1-(1,1-dimethyl-3-methylene-1,3-propanediyl) bisbenzene group at 2000 mg/kg)
Concentration of 1,1-(1,1-dimethyl-3-methylene-1,3-propanediyl) bisbenzene (mg/mL) |
Measured concentrations |
Recovery rate (%) |
||
1st |
2nd |
Mean |
||
200 |
192.2 |
196.1 |
194.2 |
97.1 |
The concentration of the preparation was within the acceptable range (100 ± 10%)
Results: (Test group 2: 1,1-(1,1-dimethyl-3-methylene-1,3-propanediyl) bisbenzene group at 2000 mg/kg)
Concentration of 1,1-(1,1-dimethyl-3-methylene-1,3-propanediyl) bisbenzene (mg/mL) |
Measured concentrations |
Recovery rate (%) |
||
1st |
2nd |
Mean |
||
200 |
194.6 |
186.4 |
190.5 |
95.3 |
The concentration of the preparation was within the acceptable range (100 ± 10%)
Results: (Test group 3: 1,1-(1,1-dimethyl-3-methylene-1,3-propanediyl) bisbenzene group at 300 mg/kg)
Concentration of 1,1-(1,1-dimethyl-3-methylene-1,3-propanediyl) bisbenzene (mg/mL) |
Measured concentrations |
Recovery rate (%) |
||
1st |
2nd |
Mean |
||
30 |
28.77 |
28.78 |
28.78 |
95.9 |
The concentration of the preparation was within the acceptable range (100 ± 10%)
Results: (Test group 4: 1,1-(1,1-dimethyl-3-methylene-1,3-propanediyl) bisbenzene group at 300 mg/kg)
Concentration of 1,1-(1,1-dimethyl-3-methylene-1,3-propanediyl) bisbenzene (mg/mL) |
Measured concentrations |
Recovery rate (%) |
||
1st |
2nd |
Mean |
||
30 |
29.88 |
29.31 |
29.60 |
98.7 |
The concentration of the preparation was within the acceptable range (100 ± 10%)
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The LD50 value of 2,4-diphenyl-4-methyl-1-pentene is estimated to be between 300 mg/kg and 2000 mg/kg. Based on the results the test substance should be classified as Acute Toxicity Category 4 according to CLP regulation 1272/2008
Respiratory paralysis or central nervous abnormality is thought to have led to deaths because tremor and clonic convulsions were observed following the administration of the test item at 2000 mg/kg.
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