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EC number: 218-915-0 | CAS number: 2280-49-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin corrosion and irritation was tested in vitro in studies according to GLP and OECD TG 431 and 439, respectively. No corrosive or irritation property of the test compound was observed, these data are in agreement with early and limited documented studies in rabbits and humans in which cotton patch tests did not indicate irritation properties to the skin. Overall, no skin irritation property is anticipated.
Eye irritation was tested in an in vitro human corneal epithelial cell (HCE) test according to GLP. No irritation property was observed. In an early, limited and only shortly reported in vivo test in rabbits only very slight and transient effects were observed. Overall, it is concluded that the compound has no eye irritation properties.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study according to GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Species:
- other: in vitro
- Strain:
- other: reconstructed human epidermis (RHS) model EST-1000
- Type of coverage:
- other: in vitro
- Preparation of test site:
- other: in vitro
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: The results of the concurrent negative control (NC, 0.9% NaCI) and positive control (PC, 5% SDS) demonstrated the viability (NC) and sensitivity (PC) of the test model.
- Duration of treatment / exposure:
- Undiluted N-Phenyi-N-[(trichloromethyl)thio]benzenesulphonamide was applied topically to the RHS model, i.e. 30 mg per insert (plus 30 µl 0.9%NaCI to moisten and ensure good contact with the skin; three replicates).
- Observation period:
- After an exposure period of 20 minutes, followed by a 42 hours post-treatment incubation period, the cell viability was measured to be 102 % (rounded) in the MTT (Methylthiazoletetrazolium) conversion assay.
- Number of animals:
- not applicable; triplicates
- Irritation / corrosion parameter:
- other: % cell viability
- Run / experiment:
- Cell viability [%]
- Value:
- 102.38
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Non-Irritant
- Interpretation of results:
- GHS criteria not met
- Executive summary:
Thus, the results show that no skin irritant property of N-Phenyl-N[( trichloromethyl)thio]benzenesulphonamide was determined by the assay used.
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study according to GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Species:
- other: in vitro
- Strain:
- other: The experiment was carried out on a reconstructed human epidermis EST-1000 (CeiiSystems, St. Katharinen, Germany)
- Type of coverage:
- other:
- Preparation of test site:
- other:
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Duration of treatment / exposure:
- A 100% concentration was tested on the skin/epidermal equivalents in triplets. For the determination of time related cytotoxic effects the incubation periods were 3 min. and 60 min., respectively.
- Observation period:
- 3 min. and 60 min.
- Number of animals:
- in vitro;n triplicates
- Irritation / corrosion parameter:
- other: % cell viability
- Run / experiment:
- Cell viability after 3 min. [%]
- Value:
- 107
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Non-Corrosive
- Irritation / corrosion parameter:
- other: % cell viability
- Run / experiment:
- Cell viability after 60 min. [%]
- Value:
- 100.91
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Non-Corrosive
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Thus, the results show that no corrosive property of the test item was determined by the assay used.
Referenceopen allclose all
After an exposure period of 20 minutes, followed by a 42 hours post-treatment incubation period, the cell viability was measured to be 102 % (rounded) in the MTT (Methylthiazoletetrazolium) conversion assay.
The results of the concurrent negative control (NC, 0.9% NaCI) and positive control (PC, 5% SDS) demonstrated the viability (NC) and sensitivity (PC) of the test model.
Thus, the results show that no skin irritant property of N-Phenyi-N[( trichloromethyl)thio]benzenesulphonamide was determined by the assay used.
The MTT (Methylthiazoletetrazolium) method has determined the following values of viability after 3 min. or after 60 min. of incubation: 107% and 101% (rounded), respectively. Thus, the results show that no corrosive property of the test item was
determined by the assay used.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study according to GLP
- Principles of method if other than guideline:
- The HCE model is currently involved in the eye irritation validation conducted by the COLIPA following ECVAM guidelines. This study is expected to end late this year and results published early 2012. The HCE is produced and commercialized by SkinEthic since 2000- more than 11 years-, and is the only model made from human corneal cells. The model is routinely used by the major Cosmetic and Pharmaceutical companies, and has already been prevalidated in 2004 (4). Furthermore, this model is recognized as the model of choice and scientifically relevant as documented by several publications (5, 6, 7). These tests are also related to OECD 405, Attachment March 2000.
The purpose of this protocol is to evaluate the potential ocular irritation of the test substance by measuring cell viability in the human corneal epithelial cell (HCE) construct, available from SkinEthic Laboratories, following topical exposure to the test substance via MTT [3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide] dye conversion. - GLP compliance:
- yes (incl. QA statement)
- Species:
- other: in vitro
- Strain:
- other: human corneal epithelial cell (HCE)
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: The results of the concurrent negative (NC, PBS) and positive control (PC, 1 H-1 ,2,4-Triazole-3-thiol) demonstrated the viability (NC) and sensitivity (PC) of the test model.
- Duration of treatment / exposure:
- After an exposure period of 60 minutes, followed by a 16 hours post-treatment incubation period, the cell viability was 93% (rounded) as measured by a MTT conversion assay.
- Observation period (in vivo):
- After an exposure period of 60 minutes, followed by a 16 hours post-treatment incubation period, the cell viability was 93% (rounded) as measured by a MTT conversion assay.
- Number of animals or in vitro replicates:
- in vitro; triplicates
- Irritation parameter:
- other: % cell viability
- Run / experiment:
- Cell viability [%]
- Value:
- 93.17
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Non-Irritant
- Interpretation of results:
- GHS criteria not met
- Executive summary:
The results show that N-Phenyl-N-[(trichloromethyl)thio]benzenesulphonamide is predicted as non-irritant under the conditions of this test method.
Reference
After an exposure period of 60 minutes, followed by a 16 hours post-treatment incubation period, the cell viability was 93% (rounded) as measured by a MTT conversion assay.
The results of the concurrent negative (NC, PBS) and positive control (PC, 1 H-1 ,2,4-Triazole-3-thiol) demonstrated the viability (NC) and sensitivity (PC) of the
test model.
The results show that N-Phenyi-N-[(trichloromethyl)thio]benzenesulphonamide is predicted as non-irritant under the conditions of this test method.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for selection of skin irritation / corrosion endpoint:
Most reliable study; additional weight-of-evidence studies are
discussed in the key information.
Justification for selection of eye irritation endpoint:
Most reliable study; an additional in vivo study is discussed on a
weight of evidence base in the key information.
Justification for classification or non-classification
No skin or eye irritation properties are anticipated based on reliable in vitro data and limited in vivo data.
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