Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 240-400-4 | CAS number: 16324-27-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Endpoint summary
Administrative data
Description of key information
Short-term
Fish: LC50 (96 h) > 1000 mg/l
Aquatic invertebrates: EC50 48h > 113 mg/l
Algae: ErC50 (72hrs) = ErC50 (72h) > 123 mg/l (nominal); NOEC (72h, growth rate) = 30.8 mg/l (nominal)
Long-term toxicity
Fish: NOEC (fry length, fry weight) = 4.41 mg/l (TWA, mean measured)
Aquatic invertebrates: NOEC (21d, overall threshold effects) = 1.10 mg/l (nominal)
Additional information
All three acute trophic levels were assessed either with study on the substance itself or with reliable Read Across studies on analogous substances. Justification for Read Across is given in Section 13 of IUCLID. EC50 was always measured as higher than 100 mg/l.
For the short-term toxicity to fish, some summary reports are available, for which the original complete reports were not recovered. The summaries are old, but they contain enough details to well identify the studies. They are all consistent and indicate a LC50 on Zebrafish at 96h higher than 500 - 1000 mg/l. A test (with a good reliability) on the toxicity to fish at 14 -days with Brachydanio rerio is available on the similar substance 3a-MSA. The nominal concentrations of 100, 316, 1000 mg /l were tested and the NOEC was identified at the concentration of 859 mg/l (nominal) (Bayer, 1992).
For the short-term toxicity to aquatic invertebrates, one test was performed on the substance under registration 3c-MSA (Ciba-Geigy Ltd in 1996), according to OECD guideline 202 on Daphnia magna; at the nominal concentration of 100 mg/l tested, no effects were observed. Nevertheless, the measured concentrations after 48h were less than 20 % of the nominal and geometric mean will result in EC 50 > 53 mg/l.
Acute toxicity for Daphnia magna was evaluated for the analogue substance 3a-MSA in a 48 hours screening test, according to the OECD guideline 202. A single dose was administrated at 100 mg/l (limit test) with no effect. The result was confirmed by two supporting studies: one performed at just 24h (RCC Umweltchemie AG., 1988) and the other one at 48, but just the summary is available (Solvias AG., 1999), with no effect up to 1000 mg/l.
No short-term toxicity to aquatic invertebrate is expected also for 3c-MSA. The result of the key study for 3a-MSA is taken as a reference for risk assessment.
The toxicity of the substance to aquatic algae was evaluated by considering data on similar substances. Supporting data from studies on the substance itself is also taken into consideration.
The substance is part of the group 3, but it has a difference in the organic substitution moiety: it has just one functionality hydroxyethyl, instead then two, as amino substituent.
It is a medium sulfonated, and the performed supporting study on the same substance indicate a NOEC < 100 mg/l. It has also to be noticed that water solubility is much less than the water solubility of the analogue 3a-MSA. In this respect the most similar and representative substance cannot be identified in the same group as 3a-MSA, but in group 2 (2-MSA) and 4 (4-MSA).
Regarding long-term studies, a long-term toxicity study on fish and on aquatic invertebrates are present for the similar substance 1 -MSA, performed according to the OECD Guideline 210 and 211, respectively and under GLP conditions.
The test item caused significant effects on Zebrafish in an early life stage test, 30 days post hatch. For the parameter hatch, the NOEC was 10.0 mg/l. Therefore, the respective LOEC was determined to be > 10.0 mg/l. For the parameters post hatch survival and overall survival, the NOECs were 1.25 and 2.50 mg/l, respectively. Therefore, the respective LOECs were determined to be 2.50 and 5.00 mg/l. For the parameter fry growth (expressed as length and fresh weight) the NOECs were < 0.625 mg/l (length) and 0.625 mg/l (weight). Therefore, the LOECs for length and weight were determined to be 0.625 and 1.25 mg/l, respectively.
The effects on reproduction of daphnia were evaluated based on the reproduction per introduced parent animal, since this parameter is the ecologically most relevant response variable and is required by the OECD test guideline 211 (2012), when a significant trend in mortality is detected. The overall effect threshold for effects of the test item on daphnid reproduction under the test conditions was 11.3 mg/l (NOEC) and 22.5 mg/l (LOEC) based on the nominal concentrations of the test item.
Considering the available data on the three trophic levels, daphnia is considered to be the most sensitive species as the effect level during the long-term reproductive study was the lowest obtained.
Therefore, SELECTED NOEC for aquatic toxicity: 1.10 mg/l from the 21-days reproductive Daphnia study on the similar substance 1 -MSA.
The study conducted on the similar substance 1 -MSA is used for the chemical safety assessment, following a conservative approach. More information on the results of the substances of the category are found on the document attached under "Ecotoxicological information".
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)