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EC number: 235-469-2 | CAS number: 12237-63-7 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 45160:2.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- other: EEC L 133, p. 118-122
- Deviations:
- yes
- Remarks:
- Instead a centrifuged sludge a settled sludge was used.
- Principles of method if other than guideline:
- The inhibitions were calculated on the basis of the measured time depend oxygen consumption of a Blank and Test solution.
- GLP compliance:
- yes
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Test system : Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2°C
- Nominal and measured concentrations:
- TEST CONCENTRATIONS
- Test concentrations:
Reference substance : 32, 10 and 3.2 mg/l
Test substance : 100.58, 32.19, 10.1, 3.22 and 1.01 mg/L
The sludge concentration in the test bottles was 1.5 g/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml BOD flasks with gas inlet
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated drinking water
Measurements : Oxygen consumption per hour in mg/liter with a ORION - Electrode Type 97-08, ORION Microprocessor lonalizer 901 and plotted on a recorder. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Graphically determined
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Graphically determined
- Duration:
- 3 h
- Dose descriptor:
- other: IC80
- Effect conc.:
- 78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Graphically determined
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: reference substance
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Graphically determined
- Details on results:
- The inhibitions were calculated on the basis of the measured time depend oxygen consumption of a Blank and Test solution.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Toxicity studies to determine the inhibitory concentration of test substance on the respiration of aerobic waste water bacteria were conducted according to EEC L 133, p. 118-122 (30. May 1988) guideline. The test was conducted on activated sludge from a sewage treatment plant for 3 hours at 20°C. The results were graphically determined based on nominal concentrations and IC50 value was determined to be 15 mg/L
- Executive summary:
Toxicity studies to determine the inhibitory concentration of test material on the respiration of aerobic waste water bacteria were conducted according to EEC L 133, p. 118-122 (30. May 1988) guideline. The test was conducted on activated sludge from a sewage treatment plant for 3 hours at 20°C. The results were graphically determined . Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. 3,5-Dichlorophenol was used as referance substance. The inhibitory concentration (IC50) value was determined to be 15 mg/l.
Reference
Table: Test result
Sample |
Concentration (mg/l) |
Consumption rate (mg/l/h) |
Inhibition percentage (%) |
Blank 1 Blank 2 Reference 1 Reference 2 Reference 3 |
0.00 0.00 32.00 10.00 3.20 |
48.75 44.66 7.90 32.03 36.11 |
-4 4 83 31 23 |
Test conc. 1 Test conc. 2 Test conc. 3 Test conc. 4 Test conc. 5 |
100.58 32.19 10.10 3.22 1.01 |
8.71 12.59 28.22 39.69 43.97 |
81 73 40 15 6 |
Description of key information
Toxicity to microorganism:
Toxicity studies to determine the inhibitory concentration of test material on the respiration of aerobic waste water bacteria were conducted according to EEC L 133, p. 118-122 (30. May 1988) guideline. The test was conducted on activated sludge from a sewage treatment plant for 3 hours at 20°C. The results were graphically determined . Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. 3,5-Dichlorophenol was used as referance substance. The inhibitory concentration (IC50) value was determined to be 15 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 15 mg/L
Additional information
Toxicity to microorganism:
Toxicity of test material on microorganism was evaluated using two experimental reports described as follows:
In the first toxicity studies to determine the inhibitory concentration of test material on the respiration of aerobic waste water bacteria were conducted according to EEC L 133, p. 118-122 (30. May 1988) guideline. The test was conducted on activated sludge from a sewage treatment plant for 3 hours at 20°C. The results were graphically determined . Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. 3,5-Dichlorophenol was used as referance substance.The inhibitory concentration (IC50) value was determined to be 15 mg/l.
Similarly in the second study, respiratory inhibition study ofactivated sludge by test material was conducted according to EC Directive 88/302/EEC - activated sludge: verificationof the inhibition of respiration;corresponds to the OECD Guidelines for Testing of Chemicals 209 and ISO Standard 8192.In the short-term respiration test, the effect of test substance on the respiration of aerobicMicroorganisms (activated sludge) after a short exposure time (30 to 180 min) were investigated.As the test result, the effective concentrations are determined, in which the breathinginhibited relative to a reference value without Priifsubstanz by 20, 50 and 80% (EC20,EC50, EC80). Effect of the test substance on the respiration of municipal activated sludge were found asEC20: after 30 min about 40 mg / l ,EC50: - After 30 min> 1000 mg /l, respectively.
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