Potassium O-ethyl dithiocarbonate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Acceptably documented study which meets basic scientific principles Ethyl Alcohol is both reagents used in the manufacture, as well as decomposition products of xanthates. Therefore, the health effects of Ethyl Alcohol need to be considered in the assessment of potassium ethyl xanthate.

Data source

Reference

Reference Type:

publication

Title:

Detection of carcinogens as mutagens in the Salmonella/microsome terst: Assay of 300 chemicals.

Author:

McCann, J., Choi, E., Yamasaki, E., Ames, B.N.

Year:

1975

Bibliographic source:

Proc Nat Acad Sci 1975;72(12):5135-5139.

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

only two bacterial strains tested.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Ethanol

EC Number:

200-578-6

EC Name:

Ethanol

Cas Number:

64-17-5

Molecular formula:

C2H6O

IUPAC Name:

ethanol

Constituent 2

Reference substance name:

Ethyl Alcohol

IUPAC Name:

Ethyl Alcohol

Test material form:

other: liquid

Details on test material:

Ethyl Alcohol is both reagents used in the manufacture, as well as decomposition products of xanthates.
-Name of test material (as cited in study report):Ethyl Alcohol.Therefore, the health effects of Ethyl Alcohol need to be considered in the assessment of potassium ethyl xanthate.
-described as 'commercial solvent'.

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 98

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 100

Metabolic activation:

with

Metabolic activation system:

rat liver S9

Test concentrations with justification for top dose:

Not specified for this compound but typically 10, 100, 500, 1000 microgram/plate. (10,000 known also to have been tested).

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

benzo(a)pyrene

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

There were < 70 revertants per 10mg/plate tested.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'. Remarks: Salmonella/microsome

Any other information on results incl. tables

There were < 70 revertants per 10mg/plate tested.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

As part of a study of the mutagenic potential of 300 chemicals, ethanol was evaluated in a bacterial reverse mutation (Ames) assay at a plate concentration of up to 10mg/plate in the presence of metabolic activation. There was no evidence of mutagenicity in either of the two strains examined (TA98, TA100). . It should be noted that only two of the normal four bacterial strains were evaluated, so the study cannot be regarded as complete or definitive.
Ethyl Alcohol is both reagents used in the manufacture, as well as decomposition products of xanthates. Therefore, the health effects of Ethyl Alcohol need to be considered in the assessment of potassium ethyl xanthate.

Executive summary:

As part of a study of the mutagenic potential of 300 chemicals, ethanol was evaluated in a bacterial reverse mutation (Ames) assay at a plate concentration of up to 10mg/plate in the presence of metabolic activation. There was no evidence of mutagenicity in either of the two strains examined (TA98, TA100). . It should be noted that only two of the normal four bacterial strains were evaluated, so the study cannot be regarded as complete or definitive.

Ethyl Alcohol is both reagents used in the manufacture, as well as decomposition products of xanthates. Therefore, the health effects of Ethyl Alcohol need to be considered in the assessment of potassium ethyl xanthate.