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EC number: 206-055-9 | CAS number: 298-06-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline compliant study, fulfilling the validity criteria determined by the guideline.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3454-66-8
- Cas Number:
- 3454-66-8
- IUPAC Name:
- 3454-66-8
- Reference substance name:
- Phosphorodithionic acid, O,O-diethyl ester, potassium salt
- IUPAC Name:
- Phosphorodithionic acid, O,O-diethyl ester, potassium salt
- Details on test material:
- - Name of test material (as cited in study report): EP-1 potassium salt
- Molecular formula (if other than submission substance): C4H10KO2PS2
- Molecular weight (if other than submission substance): 224.32 g/mol
- Physical state: white powder
- Analytical purity: 99.7% w/w
- Lot/batch No.: 343-BSe-66E
- Storage condition of test material: at -20°C
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
-Water solubility: 80 g/L
- log Pow: -0.46
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The stock solution was prepared by dissolving 487 mg and 453 mg of EP-1 potassium salt in the test medium (natural seawater) by flushing the amount into a 500 mL volumetric flask and making up to volume with filtered clean seawater, followed by mixing this solution by magnetic stirring. After 2 hours, this solution was used for preparing a dilution series with the following final concentrations as used in the test: 100, 200, 500, 1000 and 1800 mg/L.
Test organisms
- Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- TEST ORGANISM
- Strain: Clone NIVA BAC 1
Study design
- Test type:
- not specified
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- Not applicable
- Test temperature:
- 18.8 +/- 0.2°C
- pH:
- 8.1
- Dissolved oxygen:
- Not applicable
- Salinity:
- 28%
- Nominal and measured concentrations:
- nominal: 0, 100, 200, 500, 1000 and 1800 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks with total capacity of 250 mL
- Fill volume: 70 mL test solution consisting of algal growth medium, algae and test substance
- Initial cells density: 1200 cells/mL
- No. of vessels per concentration (replicates): 3
GROWTH MEDIUM
- prepared from natural seawater; heated to 73°C and filtered through Millipore filters (10, 5, 0.5 and 0.22 µm); nutrients and salts were added according to OECD 201.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: seawater - Reference substance (positive control):
- yes
- Remarks:
- 3,5-DCP
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 026 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 800 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 800 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Results with reference substance (positive control):
- The results of the reference substane 3,5-DCP with an EC50 value of 1.7 mg/L showed that the sensitivity of the algae was not significantly different from the results obtained in an international ring test (EC50r = 1.0 - 2.2 mg/L).
- Reported statistics and error estimates:
- Not applicable
Any other information on results incl. tables
The validation criteria of the test according to ISO 10253 are considered fulfilled:
- The cell density in the negative control (without test substance) increased by a factor of more than 16 during the test period.
- pH in the control flasks did not vary more than +/- 1 unit during the test.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC and LOEC for the growth rate of Skeletonema costatum after application of EP-1 potassium salt were 1000 mg/L and 1800 mg/L, respectively. The EC10 and EC50 values derived in the experiment were 1026 mg/L and > 1800 mg/L, respectively.
- Executive summary:
The test substance EP-1 potassium salt was tested for inhibitory effects on the growth of the marine microalgae Skeletonema costatum according to OECD guideline 201 and ISO 10253.
The test was carried out in 250 mL flasks with approximately 70 mL of test solution per flask. The growth of the algae was measured as fluorescence every 24 hours during the test. The total exposure time was 72 hours.
Chemical analysis revealed that the actual concentration of the stock solution was 1850 mg/L, which is close to the nominal concentration of 1880 mg/L.
The results of the study expressed in nominal concentrations can be summarized as follows:
The NOEC and LOEC for the growth rate were 1000 mg/L and 1800 mg/L, respectively and the EC10 and EC50 values for the growth rate were 1026 mg/L and >1800 mg/L, respectively.
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